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Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy

Ischemic heart disease is a leading cause of mortality and morbidity worldwide. We previously demonstrated that acacetin protects against myocardial ischemia reperfusion injury in rats, although the underlying mechanism remains to be elucidated. In the present study, we investigated the effects of a...

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Autores principales: Liu, Chong, Zhang, Minmin, Ye, Shenyi, Hong, Chenliang, Chen, Jiaxi, Lu, Ruyue, Hu, Bingjie, Yang, Weijun, Shen, Bo, Gu, Zhengyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263275/
https://www.ncbi.nlm.nih.gov/pubmed/34307696
http://dx.doi.org/10.1155/2021/9979843
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author Liu, Chong
Zhang, Minmin
Ye, Shenyi
Hong, Chenliang
Chen, Jiaxi
Lu, Ruyue
Hu, Bingjie
Yang, Weijun
Shen, Bo
Gu, Zhengyi
author_facet Liu, Chong
Zhang, Minmin
Ye, Shenyi
Hong, Chenliang
Chen, Jiaxi
Lu, Ruyue
Hu, Bingjie
Yang, Weijun
Shen, Bo
Gu, Zhengyi
author_sort Liu, Chong
collection PubMed
description Ischemic heart disease is a leading cause of mortality and morbidity worldwide. We previously demonstrated that acacetin protects against myocardial ischemia reperfusion injury in rats, although the underlying mechanism remains to be elucidated. In the present study, we investigated the effects of acacetin on autophagy during hypoxia/reoxygenation (H/R) injury by exposing H9c2 myocardial cells to H/R with or without acacetin pretreatment during hypoxia. Our results show that acacetin significantly increased cell viability in a dose-dependent manner, enhanced antioxidant capacity, and suppressed protein apoptosis of rat cardiomyocytes H9c2 cells following H/R injury. In addition, lentiviral infection of H9c2 cardiomyocytes revealed that acacetin pretreatment significantly enhanced the fluorescence intensity of autophagy proteins Beclin 1, LC3-II, and p62. These results indicate that acacetin protected H9c2 cardiomyocytes from H/R damage by enhancing autophagy. Moreover, we found that application of acacetin increased activation of the PI3K/Akt signaling pathway, whereas cotreatment with the PI3K inhibitor LY294002 reversed the inhibition of apoptosis and autophagy induced by acacetin. In conclusion, acacetin mitigated H/R injury by promoting autophagy through activating the PI3K/Akt/mTOR signaling pathway.
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spelling pubmed-82632752021-07-22 Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy Liu, Chong Zhang, Minmin Ye, Shenyi Hong, Chenliang Chen, Jiaxi Lu, Ruyue Hu, Bingjie Yang, Weijun Shen, Bo Gu, Zhengyi J Immunol Res Research Article Ischemic heart disease is a leading cause of mortality and morbidity worldwide. We previously demonstrated that acacetin protects against myocardial ischemia reperfusion injury in rats, although the underlying mechanism remains to be elucidated. In the present study, we investigated the effects of acacetin on autophagy during hypoxia/reoxygenation (H/R) injury by exposing H9c2 myocardial cells to H/R with or without acacetin pretreatment during hypoxia. Our results show that acacetin significantly increased cell viability in a dose-dependent manner, enhanced antioxidant capacity, and suppressed protein apoptosis of rat cardiomyocytes H9c2 cells following H/R injury. In addition, lentiviral infection of H9c2 cardiomyocytes revealed that acacetin pretreatment significantly enhanced the fluorescence intensity of autophagy proteins Beclin 1, LC3-II, and p62. These results indicate that acacetin protected H9c2 cardiomyocytes from H/R damage by enhancing autophagy. Moreover, we found that application of acacetin increased activation of the PI3K/Akt signaling pathway, whereas cotreatment with the PI3K inhibitor LY294002 reversed the inhibition of apoptosis and autophagy induced by acacetin. In conclusion, acacetin mitigated H/R injury by promoting autophagy through activating the PI3K/Akt/mTOR signaling pathway. Hindawi 2021-06-29 /pmc/articles/PMC8263275/ /pubmed/34307696 http://dx.doi.org/10.1155/2021/9979843 Text en Copyright © 2021 Chong Liu et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Liu, Chong
Zhang, Minmin
Ye, Shenyi
Hong, Chenliang
Chen, Jiaxi
Lu, Ruyue
Hu, Bingjie
Yang, Weijun
Shen, Bo
Gu, Zhengyi
Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy
title Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy
title_full Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy
title_fullStr Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy
title_full_unstemmed Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy
title_short Acacetin Protects Myocardial Cells against Hypoxia-Reoxygenation Injury through Activation of Autophagy
title_sort acacetin protects myocardial cells against hypoxia-reoxygenation injury through activation of autophagy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263275/
https://www.ncbi.nlm.nih.gov/pubmed/34307696
http://dx.doi.org/10.1155/2021/9979843
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