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Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions

The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the v...

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Autores principales: Connolly, Sinead, Newport, David, McGourty, Kieran
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263586/
https://www.ncbi.nlm.nih.gov/pubmed/34234155
http://dx.doi.org/10.1038/s41598-021-91865-1
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author Connolly, Sinead
Newport, David
McGourty, Kieran
author_facet Connolly, Sinead
Newport, David
McGourty, Kieran
author_sort Connolly, Sinead
collection PubMed
description The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T-cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes.
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spelling pubmed-82635862021-07-09 Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions Connolly, Sinead Newport, David McGourty, Kieran Sci Rep Article The influence of Poiseuille flow on cell viability has applications in the areas of cancer metastasis, lab-on-a-chip devices and flow cytometry. Indeed, retaining cell viability is important in the emerging field of adoptive cell therapy, as cells need to be returned to patients’ bodies, while the viability of other cells, which are perhaps less accustomed to suspension in a fluidic environment, is important to retain in flow cytometers and other such devices. Despite this, it is unclear how Poiseuille flow affects cell viability. Following on from previous studies which investigated the viability and inertial positions of circulating breast cancer cells in identical flow conditions, this study investigated the influence that varying flow rate, and the corresponding Reynolds number has on the viability of a range of different circulating cells in laminar pipe flow including primary T-cells, primary fibroblasts and neuroblastoma cells. It was found that Reynolds numbers as high as 9.13 had no effect on T-cells while the viabilities of neuroblastoma cells and intestinal fibroblasts were significantly reduced in comparison. This indicates that in vitro flow devices need to be tailored to cell-specific flow regimes. Nature Publishing Group UK 2021-07-07 /pmc/articles/PMC8263586/ /pubmed/34234155 http://dx.doi.org/10.1038/s41598-021-91865-1 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Connolly, Sinead
Newport, David
McGourty, Kieran
Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_full Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_fullStr Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_full_unstemmed Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_short Cell specific variation in viability in suspension in in vitro Poiseuille flow conditions
title_sort cell specific variation in viability in suspension in in vitro poiseuille flow conditions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8263586/
https://www.ncbi.nlm.nih.gov/pubmed/34234155
http://dx.doi.org/10.1038/s41598-021-91865-1
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