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Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems

P48/45 is a conserved gametocyte antigen involved in Plasmodium parasite fertilization. A recombinant Plasmodium vivax P48/45 (Pvs48/45) protein expressed in Escherichia coli (E. coli) was highly antigenic and immunogenic in experimental animals and elicited specific transmission-blocking (TB) antib...

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Autores principales: Arévalo-Herrera, Myriam, Miura, Kazutoyo, Cespedes, Nora, Echeverry, Carlos, Solano, Eduardo, Castellanos, Angélica, Ramirez, Juan Sebastián, Miranda, Adolfo, Kajava, Andrey V., Long, Carole, Corradin, Giampietro, Herrera, Sócrates
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264144/
https://www.ncbi.nlm.nih.gov/pubmed/34248932
http://dx.doi.org/10.3389/fimmu.2021.634738
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author Arévalo-Herrera, Myriam
Miura, Kazutoyo
Cespedes, Nora
Echeverry, Carlos
Solano, Eduardo
Castellanos, Angélica
Ramirez, Juan Sebastián
Miranda, Adolfo
Kajava, Andrey V.
Long, Carole
Corradin, Giampietro
Herrera, Sócrates
author_facet Arévalo-Herrera, Myriam
Miura, Kazutoyo
Cespedes, Nora
Echeverry, Carlos
Solano, Eduardo
Castellanos, Angélica
Ramirez, Juan Sebastián
Miranda, Adolfo
Kajava, Andrey V.
Long, Carole
Corradin, Giampietro
Herrera, Sócrates
author_sort Arévalo-Herrera, Myriam
collection PubMed
description P48/45 is a conserved gametocyte antigen involved in Plasmodium parasite fertilization. A recombinant Plasmodium vivax P48/45 (Pvs48/45) protein expressed in Escherichia coli (E. coli) was highly antigenic and immunogenic in experimental animals and elicited specific transmission-blocking (TB) antibodies in a previous pilot study. Here, a similar Pvs48/45 gene was expressed in Chinese Hamster Ovary (CHO) cells and we compared its immunoreactivity with the E. coli product. Specific antibody titers were determined using plasma from Colombian individuals (n=227) living in endemic areas where both P. vivax and P. falciparum are prevalent and from Guatemala (n=54) where P. vivax is highly prevalent. In Colombia, plasma seroprevalence to CHO-rPvs48/45 protein was 46.3%, while for E. coli-rPvs48/45 protein was 36.1% (p<0.001). In Guatemala, the sero prevalence was 24.1% and 14.8% (p<0.001), respectively. Reactivity index (RI) against both proteins showed an age-dependent increase. IgG2 was the predominant subclass and the antibody avidity index evaluated by ELISA ranged between 4-6 mol/L. Ex vivo P. vivax mosquito direct membrane feeding assays (DMFA) performed in presence of study plasmas, displayed significant parasite transmission-blocking (TB), however, there was no direct correlation between antibody titers and oocysts transmission reduction activity (%TRA). Nevertheless, DMFA with CHO rPvs48/45 affinity purified IgG showed a dose response; 90.2% TRA at 100 μg/mL and 71.8% inhibition at 10 μg/mL. In conclusion, the CHO-rPvs48/45 protein was more immunoreactive in most of the malaria endemic places studied, and CHO-rPvs48/45 specific IgG showed functional activity, supporting further testing of the protein vaccine potential.
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spelling pubmed-82641442021-07-09 Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems Arévalo-Herrera, Myriam Miura, Kazutoyo Cespedes, Nora Echeverry, Carlos Solano, Eduardo Castellanos, Angélica Ramirez, Juan Sebastián Miranda, Adolfo Kajava, Andrey V. Long, Carole Corradin, Giampietro Herrera, Sócrates Front Immunol Immunology P48/45 is a conserved gametocyte antigen involved in Plasmodium parasite fertilization. A recombinant Plasmodium vivax P48/45 (Pvs48/45) protein expressed in Escherichia coli (E. coli) was highly antigenic and immunogenic in experimental animals and elicited specific transmission-blocking (TB) antibodies in a previous pilot study. Here, a similar Pvs48/45 gene was expressed in Chinese Hamster Ovary (CHO) cells and we compared its immunoreactivity with the E. coli product. Specific antibody titers were determined using plasma from Colombian individuals (n=227) living in endemic areas where both P. vivax and P. falciparum are prevalent and from Guatemala (n=54) where P. vivax is highly prevalent. In Colombia, plasma seroprevalence to CHO-rPvs48/45 protein was 46.3%, while for E. coli-rPvs48/45 protein was 36.1% (p<0.001). In Guatemala, the sero prevalence was 24.1% and 14.8% (p<0.001), respectively. Reactivity index (RI) against both proteins showed an age-dependent increase. IgG2 was the predominant subclass and the antibody avidity index evaluated by ELISA ranged between 4-6 mol/L. Ex vivo P. vivax mosquito direct membrane feeding assays (DMFA) performed in presence of study plasmas, displayed significant parasite transmission-blocking (TB), however, there was no direct correlation between antibody titers and oocysts transmission reduction activity (%TRA). Nevertheless, DMFA with CHO rPvs48/45 affinity purified IgG showed a dose response; 90.2% TRA at 100 μg/mL and 71.8% inhibition at 10 μg/mL. In conclusion, the CHO-rPvs48/45 protein was more immunoreactive in most of the malaria endemic places studied, and CHO-rPvs48/45 specific IgG showed functional activity, supporting further testing of the protein vaccine potential. Frontiers Media S.A. 2021-06-24 /pmc/articles/PMC8264144/ /pubmed/34248932 http://dx.doi.org/10.3389/fimmu.2021.634738 Text en Copyright © 2021 Arévalo-Herrera, Miura, Cespedes, Echeverry, Solano, Castellanos, Ramirez, Miranda, Kajava, Long, Corradin and Herrera https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Arévalo-Herrera, Myriam
Miura, Kazutoyo
Cespedes, Nora
Echeverry, Carlos
Solano, Eduardo
Castellanos, Angélica
Ramirez, Juan Sebastián
Miranda, Adolfo
Kajava, Andrey V.
Long, Carole
Corradin, Giampietro
Herrera, Sócrates
Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems
title Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems
title_full Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems
title_fullStr Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems
title_full_unstemmed Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems
title_short Immunoreactivity of Sera From Low to Moderate Malaria-Endemic Areas Against Plasmodium vivax rPvs48/45 Proteins Produced in Escherichia coli and Chinese Hamster Ovary Systems
title_sort immunoreactivity of sera from low to moderate malaria-endemic areas against plasmodium vivax rpvs48/45 proteins produced in escherichia coli and chinese hamster ovary systems
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264144/
https://www.ncbi.nlm.nih.gov/pubmed/34248932
http://dx.doi.org/10.3389/fimmu.2021.634738
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