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A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis
A detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced peptides...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264744/ https://www.ncbi.nlm.nih.gov/pubmed/34278334 http://dx.doi.org/10.1016/j.xpro.2021.100648 |