Cargando…
A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis
A detailed quantification of antigen processing by endosomal compartments provides important information on the pattern of protein fragmentation. Here, we describe a protocol that combines gradient purified endosomes, incubated with antigens, followed by hot spot analysis of MS/MS-sequenced peptides...
| Autores principales: | , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264744/ https://www.ncbi.nlm.nih.gov/pubmed/34278334 http://dx.doi.org/10.1016/j.xpro.2021.100648 |