Cargando…

Identification, Characterization, and Site-Specific Mutagenesis of a Thermostable ω-Transaminase from Chloroflexi bacterium

[Image: see text] In the present study, we have identified an ω-transaminase (ω-TA) from Chloroflexi bacterium from the genome database by using two ω-TA sequences (ATA117 Arrmut11 from Arthrobacter sp. KNK168 and amine transaminase from Aspergillus terreus NIH2624) as templates in a BLASTP search a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Wang, Chen, Tang, Kexin, Dai, Ya, Jia, Honghua, Li, Yan, Gao, Zhen, Wu, Bin
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2021
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8264935/ https://www.ncbi.nlm.nih.gov/pubmed/34250363 http://dx.doi.org/10.1021/acsomega.1c02164

Ejemplares similares

Immobilization of ω-transaminase by magnetic PVA-Fe(3)O(4) nanoparticles
por: Jia, Honghua, et al.
Publicado: (2016)

A Machine Learning Study on the Thermostability Prediction of (R)-ω-Selective Amine Transaminase from Aspergillus terreus
por: Jia, Li-li, et al.
Publicado: (2021)

Structural basis of substrate recognition by a novel thermostable (S)-enantioselective ω-transaminase from Thermomicrobium roseum
por: Kwon, Sunghark, et al.
Publicado: (2019)

Robust ω-Transaminases by Computational Stabilization of the Subunit Interface
por: Meng, Qinglong, et al.
Publicado: (2020)

Computational Prediction of ω-Transaminase Specificity by a Combination of Docking and Molecular Dynamics Simulations
por: Ramírez-Palacios, Carlos, et al.
Publicado: (2021)

Quantum Chemical Study of Dual-Substrate Recognition in ω-Transaminase
por: Manta, Bianca, et al.
Publicado: (2017)

Active-site engineering of ω-transaminase from Ochrobactrum anthropi for preparation of L-2-aminobutyric acid
por: Zhang, Zhiwei, et al.
Publicado: (2021)

Improving the thermostability of GH49 dextranase AoDex by site-directed mutagenesis
por: Wei, Zhen, et al.
Publicado: (2023)

Improving the Thermostability and Activity of Transaminase From Aspergillus terreus by Charge-Charge Interaction
por: Cao, Jia-Ren, et al.
Publicado: (2021)

Crystal Structure of an (R)-Selective ω-Transaminase from Aspergillus terreus
por: Łyskowski, Andrzej, et al.
Publicado: (2014)

Improving the thermostability of alpha-amylase by combinatorial coevolving-site saturation mutagenesis
por: Wang, Chenghua, et al.
Publicado: (2012)

A Single Mutation Increases the Thermostability and Activity of Aspergillus terreus Amine Transaminase
por: Zhu, Wan-Li, et al.
Publicado: (2019)

Magnetic Catechol-Chitosan with Bioinspired Adhesive Surface: Preparation and Immobilization of ω-Transaminase
por: Ni, Kefeng, et al.
Publicado: (2012)

Structural studies reveal flexible roof of active site responsible for ω-transaminase CrmG overcoming by-product inhibition
por: Xu, Jinxin, et al.
Publicado: (2020)

Nitrification expanded: discovery, physiology and genomics of a nitrite-oxidizing bacterium from the phylum Chloroflexi
por: Sorokin, Dimitry Y, et al.
Publicado: (2012)

Phenotypical characterisation of a putative ω-amino acid transaminase in the yeast Scheffersomyces stipitis
por: Linder, Tomas
Publicado: (2018)

Enhancing PLP-Binding Capacity of Class-III ω-Transaminase by Single Residue Substitution
por: Roura Padrosa, David, et al.
Publicado: (2019)

Improved Stability and Catalytic Efficiency of ω-Transaminase in Aqueous Mixture of Deep Eutectic Solvents
por: Wang, Hongpeng, et al.
Publicado: (2023)

Computational prediction of ω-transaminase selectivity by deep learning analysis of molecular dynamics trajectories
por: Ramírez-Palacios, Carlos, et al.
Publicado: (2022)

Asymmetric synthesis of a high added value chiral amine using immobilized ω-transaminases
por: Petri, Antonella, et al.
Publicado: (2019)

Bioprospecting Reveals Class III ω-Transaminases Converting Bulky Ketones and Environmentally Relevant Polyamines
por: Coscolín, Cristina, et al.
Publicado: (2019)

Parallel Interconnected Kinetic Asymmetric Transformation (PIKAT) with an Immobilized ω-Transaminase in Neat Organic Solvent
por: Böhmer, Wesley, et al.
Publicado: (2020)

Computational Redesign of an ω-Transaminase from Pseudomonas jessenii for Asymmetric Synthesis of Enantiopure Bulky Amines
por: Meng, Qinglong, et al.
Publicado: (2021)

Enhancing a Sphaerobacter thermophilus ω-transaminase for kinetic resolution of β- and γ-amino acids
por: Wegner, Uwe, et al.
Publicado: (2023)

Improving the Specific Activity and Thermostability of Psychrophilic Xylosidase AX543 by Comparative Mutagenesis
por: Pan, Kungang, et al.
Publicado: (2022)

Continuous Flow Bioamination of Ketones in Organic Solvents at Controlled Water Activity using Immobilized ω‐Transaminases
por: Böhmer, Wesley, et al.
Publicado: (2020)

Metabolic Heterogeneity, Plasticity, and Adaptation to “Glutamine Addiction” in Cancer Cells: The Role of Glutaminase and the GTωA [Glutamine Transaminase—ω-Amidase (Glutaminase II)] Pathway
por: Cooper, Arthur J. L., et al.
Publicado: (2023)

The Phylogeny, Biodiversity, and Ecology of the Chloroflexi in Activated Sludge
por: Speirs, Lachlan B. M., et al.
Publicado: (2019)

Improving the Thermostability of Rhizopus chinensis Lipase Through Site-Directed Mutagenesis Based on B-Factor Analysis
por: Jiang, Zhanbao, et al.
Publicado: (2020)

Crystallization and preliminary X-ray diffraction analysis of ω-amino acid:pyruvate transaminase from Chromobacterium violaceum
por: Sayer, Christopher, et al.
Publicado: (2007)

Chiral Amine Synthesis Using ω-Transaminases: An Amine Donor that Displaces Equilibria and Enables High-Throughput Screening**
por: Green, Anthony P, et al.
Publicado: (2014)

A Regio- and Stereoselective ω-Transaminase/Monoamine Oxidase Cascade for the Synthesis of Chiral 2,5-Disubstituted Pyrrolidines**
por: O'Reilly, Elaine, et al.
Publicado: (2014)

β-Phenylalanine Ester Synthesis from Stable β-Keto Ester Substrate Using Engineered ω-Transaminases
por: Buß, Oliver, et al.
Publicado: (2018)

Isolation and Characterization of a Thermophilic, Obligately Anaerobic and Heterotrophic Marine Chloroflexi Bacterium from a Chloroflexi-dominated Microbial Community Associated with a Japanese Shallow Hydrothermal System, and Proposal for Thermomarinilinea lacunofontalis gen. nov., sp. nov.
por: Nunoura, Takuro, et al.
Publicado: (2013)

Draft Genome Sequence of a Sulfide-Oxidizing, Autotrophic Filamentous Anoxygenic Phototrophic Bacterium, Chloroflexus sp. Strain MS-G (Chloroflexi)
por: Thiel, Vera, et al.
Publicado: (2014)

Rational Mutagenesis of Cyclodextrin Glucanotransferase at the Calcium Binding Regions for Enhancement of Thermostability
por: Goh, Poh Hong, et al.
Publicado: (2012)

Comprehensive mutagenesis to identify amino acid residues contributing to the difference in thermostability between two originally thermostable ancestral proteins
por: Akanuma, Satoshi, et al.
Publicado: (2021)

Improving the thermostability of a fungal GH11 xylanase via site-directed mutagenesis guided by sequence and structural analysis
por: Han, Nanyu, et al.
Publicado: (2017)

Thermostability enhancement of Escherichia coli phytase by error-prone polymerase chain reaction (epPCR) and site-directed mutagenesis
por: Xing, Hongguan, et al.
Publicado: (2023)

Enzymatic and chemo-enzymatic strategies to produce highly valuable chiral amines from biomass with ω-transaminases on 2D zeolites
por: Carceller, J Miguel, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_rou1lk4iq9pk47rb03aeigsjkr