pegIT - a web-based design tool for prime editing

Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse transcriptase fusion protein, is based on dual-functioning prime editing guide RNAs (pegRNAs), serving both as guide molecules and a...

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Detalles Bibliográficos
Autores principales: Anderson, Mads Valdemar, Haldrup, Jakob, Thomsen, Emil Aagaard, Wolff, Jonas Holst, Mikkelsen, Jacob Giehm
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Web Server Issue
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265180/
https://www.ncbi.nlm.nih.gov/pubmed/34060619
http://dx.doi.org/10.1093/nar/gkab427
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author Anderson, Mads Valdemar
Haldrup, Jakob
Thomsen, Emil Aagaard
Wolff, Jonas Holst
Mikkelsen, Jacob Giehm
author_facet Anderson, Mads Valdemar
Haldrup, Jakob
Thomsen, Emil Aagaard
Wolff, Jonas Holst
Mikkelsen, Jacob Giehm
author_sort Anderson, Mads Valdemar
collection PubMed
description Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse transcriptase fusion protein, is based on dual-functioning prime editing guide RNAs (pegRNAs), serving both as guide molecules and as templates carrying the desired edits. Due to such diverse functions, manual pegRNA design is a subject to error and not suited for large-scale setups. Here, we present pegIT, a user-friendly web tool for rapid pegRNA design for numerous user-defined edits, including large-scale setups. pegIT is freely available at https://pegit.giehmlab.dk.
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spelling pubmed-82651802021-07-09 pegIT - a web-based design tool for prime editing Anderson, Mads Valdemar Haldrup, Jakob Thomsen, Emil Aagaard Wolff, Jonas Holst Mikkelsen, Jacob Giehm Nucleic Acids Res Web Server Issue Prime editing (PE) is a novel CRISPR-derived genome editing technique facilitating precision editing without double-stranded DNA breaks. PE, mediated by a Cas9-reverse transcriptase fusion protein, is based on dual-functioning prime editing guide RNAs (pegRNAs), serving both as guide molecules and as templates carrying the desired edits. Due to such diverse functions, manual pegRNA design is a subject to error and not suited for large-scale setups. Here, we present pegIT, a user-friendly web tool for rapid pegRNA design for numerous user-defined edits, including large-scale setups. pegIT is freely available at https://pegit.giehmlab.dk. Oxford University Press 2021-06-01 /pmc/articles/PMC8265180/ /pubmed/34060619 http://dx.doi.org/10.1093/nar/gkab427 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Web Server Issue
Anderson, Mads Valdemar
Haldrup, Jakob
Thomsen, Emil Aagaard
Wolff, Jonas Holst
Mikkelsen, Jacob Giehm
pegIT - a web-based design tool for prime editing
title pegIT - a web-based design tool for prime editing
title_full pegIT - a web-based design tool for prime editing
title_fullStr pegIT - a web-based design tool for prime editing
title_full_unstemmed pegIT - a web-based design tool for prime editing
title_short pegIT - a web-based design tool for prime editing
title_sort pegit - a web-based design tool for prime editing
topic Web Server Issue
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265180/
https://www.ncbi.nlm.nih.gov/pubmed/34060619
http://dx.doi.org/10.1093/nar/gkab427
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