Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry

Two mechanochemical procedures for (17)O/(18)O-isotope labeling of fatty acids are reported: a carboxylic acid activation/hydrolysis approach and a saponification approach. The latter route allowed first-time enrichment of important polyunsaturated fatty acids (PUFAs) including docosahexaenoic acid...

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Detalles Bibliográficos
Autores principales: Špačková, Jessica, Fabra, Charlyn, Cazals, Guillaume, Hubert-Roux, Marie, Schmitz-Afonso, Isabelle, Goldberga, Ieva, Berthomieu, Dorothée, Lebrun, Aurélien, Métro, Thomas-Xavier, Laurencin, Danielle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265319/
https://www.ncbi.nlm.nih.gov/pubmed/34143162
http://dx.doi.org/10.1039/d1cc02165f
Descripción
Sumario:Two mechanochemical procedures for (17)O/(18)O-isotope labeling of fatty acids are reported: a carboxylic acid activation/hydrolysis approach and a saponification approach. The latter route allowed first-time enrichment of important polyunsaturated fatty acids (PUFAs) including docosahexaenoic acid (DHA). Overall, a total of 9 pure labeled products were isolated in high yields (≥80%) and with high enrichment levels (≥37% average labeling of C=O and C-OH carboxylic oxygen atoms), under mild conditions, and in short time (<half day).

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