Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry

Two mechanochemical procedures for (17)O/(18)O-isotope labeling of fatty acids are reported: a carboxylic acid activation/hydrolysis approach and a saponification approach. The latter route allowed first-time enrichment of important polyunsaturated fatty acids (PUFAs) including docosahexaenoic acid...

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Autores principales: Špačková, Jessica, Fabra, Charlyn, Cazals, Guillaume, Hubert-Roux, Marie, Schmitz-Afonso, Isabelle, Goldberga, Ieva, Berthomieu, Dorothée, Lebrun, Aurélien, Métro, Thomas-Xavier, Laurencin, Danielle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265319/
https://www.ncbi.nlm.nih.gov/pubmed/34143162
http://dx.doi.org/10.1039/d1cc02165f
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author Špačková, Jessica
Fabra, Charlyn
Cazals, Guillaume
Hubert-Roux, Marie
Schmitz-Afonso, Isabelle
Goldberga, Ieva
Berthomieu, Dorothée
Lebrun, Aurélien
Métro, Thomas-Xavier
Laurencin, Danielle
author_facet Špačková, Jessica
Fabra, Charlyn
Cazals, Guillaume
Hubert-Roux, Marie
Schmitz-Afonso, Isabelle
Goldberga, Ieva
Berthomieu, Dorothée
Lebrun, Aurélien
Métro, Thomas-Xavier
Laurencin, Danielle
author_sort Špačková, Jessica
collection PubMed
description Two mechanochemical procedures for (17)O/(18)O-isotope labeling of fatty acids are reported: a carboxylic acid activation/hydrolysis approach and a saponification approach. The latter route allowed first-time enrichment of important polyunsaturated fatty acids (PUFAs) including docosahexaenoic acid (DHA). Overall, a total of 9 pure labeled products were isolated in high yields (≥80%) and with high enrichment levels (≥37% average labeling of C=O and C-OH carboxylic oxygen atoms), under mild conditions, and in short time (<half day).
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spelling pubmed-82653192021-07-16 Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry Špačková, Jessica Fabra, Charlyn Cazals, Guillaume Hubert-Roux, Marie Schmitz-Afonso, Isabelle Goldberga, Ieva Berthomieu, Dorothée Lebrun, Aurélien Métro, Thomas-Xavier Laurencin, Danielle Chem Commun (Camb) Chemistry Two mechanochemical procedures for (17)O/(18)O-isotope labeling of fatty acids are reported: a carboxylic acid activation/hydrolysis approach and a saponification approach. The latter route allowed first-time enrichment of important polyunsaturated fatty acids (PUFAs) including docosahexaenoic acid (DHA). Overall, a total of 9 pure labeled products were isolated in high yields (≥80%) and with high enrichment levels (≥37% average labeling of C=O and C-OH carboxylic oxygen atoms), under mild conditions, and in short time (<half day). The Royal Society of Chemistry 2021-06-18 /pmc/articles/PMC8265319/ /pubmed/34143162 http://dx.doi.org/10.1039/d1cc02165f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Špačková, Jessica
Fabra, Charlyn
Cazals, Guillaume
Hubert-Roux, Marie
Schmitz-Afonso, Isabelle
Goldberga, Ieva
Berthomieu, Dorothée
Lebrun, Aurélien
Métro, Thomas-Xavier
Laurencin, Danielle
Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry
title Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry
title_full Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry
title_fullStr Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry
title_full_unstemmed Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry
title_short Cost-efficient and user-friendly (17)O/(18)O labeling procedures of fatty acids using mechanochemistry
title_sort cost-efficient and user-friendly (17)o/(18)o labeling procedures of fatty acids using mechanochemistry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8265319/
https://www.ncbi.nlm.nih.gov/pubmed/34143162
http://dx.doi.org/10.1039/d1cc02165f
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