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Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study

Latent tuberculosis infection (LTBI) is a condition that has no clinical signs and symptoms. LTBI patients are characterized by persistent immune responses to Mycobacterium tuberculosis, and approximately 5-10% of these infected individuals will develop active TB at some point in their lives. The an...

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Autores principales: Cazarez-Navarro, Gerardo, Palomares-Marín, Jaime, Rodríguez-Preciado, Sergio Yair, Pereira-Suárez, Ana Laura, Martínez-López, Erika, Bacilio-Medrano, Eva Adriana, Huerta-Olvera, Selene, Hernández-Cañaveral, Iván Isidro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto de Medicina Tropical de São Paulo 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266304/
https://www.ncbi.nlm.nih.gov/pubmed/34231820
http://dx.doi.org/10.1590/S1678-9946202163055
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author Cazarez-Navarro, Gerardo
Palomares-Marín, Jaime
Rodríguez-Preciado, Sergio Yair
Pereira-Suárez, Ana Laura
Martínez-López, Erika
Bacilio-Medrano, Eva Adriana
Huerta-Olvera, Selene
Hernández-Cañaveral, Iván Isidro
author_facet Cazarez-Navarro, Gerardo
Palomares-Marín, Jaime
Rodríguez-Preciado, Sergio Yair
Pereira-Suárez, Ana Laura
Martínez-López, Erika
Bacilio-Medrano, Eva Adriana
Huerta-Olvera, Selene
Hernández-Cañaveral, Iván Isidro
author_sort Cazarez-Navarro, Gerardo
collection PubMed
description Latent tuberculosis infection (LTBI) is a condition that has no clinical signs and symptoms. LTBI patients are characterized by persistent immune responses to Mycobacterium tuberculosis, and approximately 5-10% of these infected individuals will develop active TB at some point in their lives. The antigen transporter associated with antigen processing (TAP1) is a protein involved in the transport of the antigen from the cytoplasm to the endoplasmic reticulum by means of the association with MHC class I molecules. It plays a fundamental role in the immune response, promoting the clearance of intracellular pathogens. Our pilot study aimed to determine the association between TAP1 gene 1177A>G (rs1057141) and 2090A>G (rs1135216) genetic polymorphisms with susceptibility to LTBI. In this case-control study, 153 individuals from shelters were analyzed (46 were LTBI-positive and 92 were controls). Genotyping of the rs11352216 (2090A>G) and rs1057141 (1177A>G) gene IDs was performed using the Applied Biosystems Step One Thermal Cycler Real-Time PCR allelic discrimination technology. The haplotypic analyses were performed with the Arlequin 3.5 program. Social assistance centers and shelters that serve vulnerable populations represent high-risk sites due to overcrowding and the impaired nutritional status of their residents. The G allele (OR=1.99, CI=1.109-3.587, p=0.021) and the GG genotype of rs11352216 (A>G) were associated with susceptibility to LTBI, according to the codominant genetic model (OR=8.32, CI=1.722-61.98, p=0.007). The rs1057141 (A>G) polymorphism was not associated with LTBI risk. The results suggest that carriers of the G allele of rs1135216 (A>G) are susceptible to LTBI.
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spelling pubmed-82663042021-07-19 Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study Cazarez-Navarro, Gerardo Palomares-Marín, Jaime Rodríguez-Preciado, Sergio Yair Pereira-Suárez, Ana Laura Martínez-López, Erika Bacilio-Medrano, Eva Adriana Huerta-Olvera, Selene Hernández-Cañaveral, Iván Isidro Rev Inst Med Trop Sao Paulo Brief Communication Latent tuberculosis infection (LTBI) is a condition that has no clinical signs and symptoms. LTBI patients are characterized by persistent immune responses to Mycobacterium tuberculosis, and approximately 5-10% of these infected individuals will develop active TB at some point in their lives. The antigen transporter associated with antigen processing (TAP1) is a protein involved in the transport of the antigen from the cytoplasm to the endoplasmic reticulum by means of the association with MHC class I molecules. It plays a fundamental role in the immune response, promoting the clearance of intracellular pathogens. Our pilot study aimed to determine the association between TAP1 gene 1177A>G (rs1057141) and 2090A>G (rs1135216) genetic polymorphisms with susceptibility to LTBI. In this case-control study, 153 individuals from shelters were analyzed (46 were LTBI-positive and 92 were controls). Genotyping of the rs11352216 (2090A>G) and rs1057141 (1177A>G) gene IDs was performed using the Applied Biosystems Step One Thermal Cycler Real-Time PCR allelic discrimination technology. The haplotypic analyses were performed with the Arlequin 3.5 program. Social assistance centers and shelters that serve vulnerable populations represent high-risk sites due to overcrowding and the impaired nutritional status of their residents. The G allele (OR=1.99, CI=1.109-3.587, p=0.021) and the GG genotype of rs11352216 (A>G) were associated with susceptibility to LTBI, according to the codominant genetic model (OR=8.32, CI=1.722-61.98, p=0.007). The rs1057141 (A>G) polymorphism was not associated with LTBI risk. The results suggest that carriers of the G allele of rs1135216 (A>G) are susceptible to LTBI. Instituto de Medicina Tropical de São Paulo 2021-07-05 /pmc/articles/PMC8266304/ /pubmed/34231820 http://dx.doi.org/10.1590/S1678-9946202163055 Text en https://creativecommons.org/licenses/by-nc/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Brief Communication
Cazarez-Navarro, Gerardo
Palomares-Marín, Jaime
Rodríguez-Preciado, Sergio Yair
Pereira-Suárez, Ana Laura
Martínez-López, Erika
Bacilio-Medrano, Eva Adriana
Huerta-Olvera, Selene
Hernández-Cañaveral, Iván Isidro
Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study
title Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study
title_full Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study
title_fullStr Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study
title_full_unstemmed Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study
title_short Association of TAP1 1177A>G and 2090A>G gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of Guadalajara, Mexico: a pilot study
title_sort association of tap1 1177a>g and 2090a>g gene polymorphisms with latent tuberculosis infections in sheltered populations, in the metropolitan area of guadalajara, mexico: a pilot study
topic Brief Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266304/
https://www.ncbi.nlm.nih.gov/pubmed/34231820
http://dx.doi.org/10.1590/S1678-9946202163055
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