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Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1
In this study, we investigated the effects of calycosin on breast cancer cell progression and their underlying mechanisms. Calycosin dose- and time-dependently inhibited proliferation, migration, and invasion by T47D and MCF-7 breast cancer cells by downregulating basic leucine zipper ATF-like trans...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266341/ https://www.ncbi.nlm.nih.gov/pubmed/34096887 http://dx.doi.org/10.18632/aging.203093 |
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author | Zhang, Zhenxia Lin, Min Wang, Junli Yang, Fenglian Yang, Peikui Liu, Yaqun Chen, Zikai Zheng, Yuzhong |
author_facet | Zhang, Zhenxia Lin, Min Wang, Junli Yang, Fenglian Yang, Peikui Liu, Yaqun Chen, Zikai Zheng, Yuzhong |
author_sort | Zhang, Zhenxia |
collection | PubMed |
description | In this study, we investigated the effects of calycosin on breast cancer cell progression and their underlying mechanisms. Calycosin dose- and time-dependently inhibited proliferation, migration, and invasion by T47D and MCF-7 breast cancer cells by downregulating basic leucine zipper ATF-like transcription factor (BATF) expression. Moreover, BATF promoted breast cancer cell migration and invasiveness by increasing TGFβ1 mRNA and protein levels. Bioinformatics analysis, dual luciferase reporter assays, and chromatin immunoprecipitation assays confirmed the presence of BATF-binding sites in the promoter sequence of TGFβ1 gene. Calycosin treatment inhibited epithelial-mesenchymal transition (EMT) of breast cancer cells by significantly increasing E-cadherin levels and decreasing N-cadherin, Vimentin, CD147, MMP-2, and MMP-9 levels through downregulation of BATF and TGFβ1. TGFβ1 knockdown reduced the migration and invasiveness of BATF-overexpressing breast cancer cells, whereas incubation with TGFβ1 enhanced the migration and invasiveness of calycosin-treated breast cancer cells. Our findings demonstrated that calycosin inhibited EMT and progression of breast cancer cells by suppressing BATF/TGFβ1 signaling. This suggests calycosin would be a promising therapeutic option for breast cancer patients. |
format | Online Article Text |
id | pubmed-8266341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Impact Journals |
record_format | MEDLINE/PubMed |
spelling | pubmed-82663412021-07-09 Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1 Zhang, Zhenxia Lin, Min Wang, Junli Yang, Fenglian Yang, Peikui Liu, Yaqun Chen, Zikai Zheng, Yuzhong Aging (Albany NY) Research Paper In this study, we investigated the effects of calycosin on breast cancer cell progression and their underlying mechanisms. Calycosin dose- and time-dependently inhibited proliferation, migration, and invasion by T47D and MCF-7 breast cancer cells by downregulating basic leucine zipper ATF-like transcription factor (BATF) expression. Moreover, BATF promoted breast cancer cell migration and invasiveness by increasing TGFβ1 mRNA and protein levels. Bioinformatics analysis, dual luciferase reporter assays, and chromatin immunoprecipitation assays confirmed the presence of BATF-binding sites in the promoter sequence of TGFβ1 gene. Calycosin treatment inhibited epithelial-mesenchymal transition (EMT) of breast cancer cells by significantly increasing E-cadherin levels and decreasing N-cadherin, Vimentin, CD147, MMP-2, and MMP-9 levels through downregulation of BATF and TGFβ1. TGFβ1 knockdown reduced the migration and invasiveness of BATF-overexpressing breast cancer cells, whereas incubation with TGFβ1 enhanced the migration and invasiveness of calycosin-treated breast cancer cells. Our findings demonstrated that calycosin inhibited EMT and progression of breast cancer cells by suppressing BATF/TGFβ1 signaling. This suggests calycosin would be a promising therapeutic option for breast cancer patients. Impact Journals 2021-06-07 /pmc/articles/PMC8266341/ /pubmed/34096887 http://dx.doi.org/10.18632/aging.203093 Text en Copyright: © 2021 Zhang et al. https://creativecommons.org/licenses/by/3.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/3.0/) (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Zhang, Zhenxia Lin, Min Wang, Junli Yang, Fenglian Yang, Peikui Liu, Yaqun Chen, Zikai Zheng, Yuzhong Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1 |
title | Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1 |
title_full | Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1 |
title_fullStr | Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1 |
title_full_unstemmed | Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1 |
title_short | Calycosin inhibits breast cancer cell migration and invasion by suppressing EMT via BATF/TGF-β1 |
title_sort | calycosin inhibits breast cancer cell migration and invasion by suppressing emt via batf/tgf-β1 |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8266341/ https://www.ncbi.nlm.nih.gov/pubmed/34096887 http://dx.doi.org/10.18632/aging.203093 |
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