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Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury

PURPOSE: To characterize in vivo dendritic changes in retinal ganglion cells (RGCs) after acute (optic nerve transection, ONT) and chronic (experimental glaucoma, EG) optic nerve injury. METHODS: ONT and EG (microbead model) were carried out in Thy1-YFP mice in which the entire RGC dendritic arbor w...

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Autores principales: Henderson, Delaney C. M., Vianna, Jayme R., Gobran, John, Pierdomenico, Johnny Di, Hooper, Michele L., Farrell, Spring R. M., Chauhan, Balwantray C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Association for Research in Vision and Ophthalmology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267182/
https://www.ncbi.nlm.nih.gov/pubmed/34232261
http://dx.doi.org/10.1167/iovs.62.9.5
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author Henderson, Delaney C. M.
Vianna, Jayme R.
Gobran, John
Pierdomenico, Johnny Di
Hooper, Michele L.
Farrell, Spring R. M.
Chauhan, Balwantray C.
author_facet Henderson, Delaney C. M.
Vianna, Jayme R.
Gobran, John
Pierdomenico, Johnny Di
Hooper, Michele L.
Farrell, Spring R. M.
Chauhan, Balwantray C.
author_sort Henderson, Delaney C. M.
collection PubMed
description PURPOSE: To characterize in vivo dendritic changes in retinal ganglion cells (RGCs) after acute (optic nerve transection, ONT) and chronic (experimental glaucoma, EG) optic nerve injury. METHODS: ONT and EG (microbead model) were carried out in Thy1-YFP mice in which the entire RGC dendritic arbor was imaged with confocal fluorescence scanning laser ophthalmoscopy over two weeks in the ONT group and over two and six months, respectively, in two (groups 1 and 2) EG groups. Sholl analysis was used to quantify dendritic structure with the parameters: area under the curve (AUC), radius of the dendritic field, peak number of intersections (PI), and distance to the PI (PD). RESULTS: Dendritic changes were observed after three days post-ONT with significant decreases in all parameters at two weeks. In group 1 EG mice, mean (SD) intraocular pressure (IOP) was 15.2 (1.1) and 9.8 (0.3) mmHg in the EG and untreated contralateral eyes, respectively, with a significant corresponding decrease in AUC, PI, and PD, but not radius. In group 2 mice, the respective IOP was 13.1 (1.0) and 8.8 (0.1) mmHg, peaking at two months before trending towards baseline. Over the first two months, AUC, PI, and PD decreased significantly, with no further subsequent changes. The rates of change of the parameters after ONT was 5 to 10 times faster than in EG. CONCLUSIONS: Rapid dendritic changes occurred after ONT, while changes in EG were slower and associated with level of IOP increase. The earliest alterations were loss of inner neurites without change in dendritic field.
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spelling pubmed-82671822021-07-14 Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury Henderson, Delaney C. M. Vianna, Jayme R. Gobran, John Pierdomenico, Johnny Di Hooper, Michele L. Farrell, Spring R. M. Chauhan, Balwantray C. Invest Ophthalmol Vis Sci Glaucoma PURPOSE: To characterize in vivo dendritic changes in retinal ganglion cells (RGCs) after acute (optic nerve transection, ONT) and chronic (experimental glaucoma, EG) optic nerve injury. METHODS: ONT and EG (microbead model) were carried out in Thy1-YFP mice in which the entire RGC dendritic arbor was imaged with confocal fluorescence scanning laser ophthalmoscopy over two weeks in the ONT group and over two and six months, respectively, in two (groups 1 and 2) EG groups. Sholl analysis was used to quantify dendritic structure with the parameters: area under the curve (AUC), radius of the dendritic field, peak number of intersections (PI), and distance to the PI (PD). RESULTS: Dendritic changes were observed after three days post-ONT with significant decreases in all parameters at two weeks. In group 1 EG mice, mean (SD) intraocular pressure (IOP) was 15.2 (1.1) and 9.8 (0.3) mmHg in the EG and untreated contralateral eyes, respectively, with a significant corresponding decrease in AUC, PI, and PD, but not radius. In group 2 mice, the respective IOP was 13.1 (1.0) and 8.8 (0.1) mmHg, peaking at two months before trending towards baseline. Over the first two months, AUC, PI, and PD decreased significantly, with no further subsequent changes. The rates of change of the parameters after ONT was 5 to 10 times faster than in EG. CONCLUSIONS: Rapid dendritic changes occurred after ONT, while changes in EG were slower and associated with level of IOP increase. The earliest alterations were loss of inner neurites without change in dendritic field. The Association for Research in Vision and Ophthalmology 2021-07-07 /pmc/articles/PMC8267182/ /pubmed/34232261 http://dx.doi.org/10.1167/iovs.62.9.5 Text en Copyright 2021 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
spellingShingle Glaucoma
Henderson, Delaney C. M.
Vianna, Jayme R.
Gobran, John
Pierdomenico, Johnny Di
Hooper, Michele L.
Farrell, Spring R. M.
Chauhan, Balwantray C.
Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury
title Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury
title_full Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury
title_fullStr Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury
title_full_unstemmed Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury
title_short Longitudinal In Vivo Changes in Retinal Ganglion Cell Dendritic Morphology After Acute and Chronic Optic Nerve Injury
title_sort longitudinal in vivo changes in retinal ganglion cell dendritic morphology after acute and chronic optic nerve injury
topic Glaucoma
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267182/
https://www.ncbi.nlm.nih.gov/pubmed/34232261
http://dx.doi.org/10.1167/iovs.62.9.5
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