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Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM

Due to the limited number of organ donors, 3D printing of organs is a promising technique. Tissue engineering is increasingly using xenogeneic material for this purpose. This study was aimed at assessing the safety of decellularized porcine pancreas, together with the analysis of the risk of an unde...

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Autores principales: Klak, Marta, Łojszczyk, Ilona, Berman, Andrzej, Tymicki, Grzegorz, Adamiok-Ostrowska, Anna, Sierakowski, Maciej, Olkowski, Radosław, Szczepankiewicz, Andrzej Antoni, Kamiński, Artur, Dobrzyń, Agnieszka, Wszoła, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267664/
https://www.ncbi.nlm.nih.gov/pubmed/34209772
http://dx.doi.org/10.3390/ijms22137005
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author Klak, Marta
Łojszczyk, Ilona
Berman, Andrzej
Tymicki, Grzegorz
Adamiok-Ostrowska, Anna
Sierakowski, Maciej
Olkowski, Radosław
Szczepankiewicz, Andrzej Antoni
Kamiński, Artur
Dobrzyń, Agnieszka
Wszoła, Michał
author_facet Klak, Marta
Łojszczyk, Ilona
Berman, Andrzej
Tymicki, Grzegorz
Adamiok-Ostrowska, Anna
Sierakowski, Maciej
Olkowski, Radosław
Szczepankiewicz, Andrzej Antoni
Kamiński, Artur
Dobrzyń, Agnieszka
Wszoła, Michał
author_sort Klak, Marta
collection PubMed
description Due to the limited number of organ donors, 3D printing of organs is a promising technique. Tissue engineering is increasingly using xenogeneic material for this purpose. This study was aimed at assessing the safety of decellularized porcine pancreas, together with the analysis of the risk of an undesirable immune response. We tested eight variants of the decellularization process. We determined the following impacts: rinsing agents (PBS/NH(3)·H(2)O), temperature conditions (4 °C/24 °C), and the grinding method of native material (ground/cut). To assess the quality of the extracellular matrix after the completed decellularization process, analyses of the following were performed: DNA concentration, fat content, microscopic evaluation, proteolysis, material cytotoxicity, and most importantly, the Triton X-100 content. Our analyses showed that we obtained a product with an extremely low detergent content with negligible residual DNA content. The obtained results confirmed the performed histological and immuno-fluorescence staining. Moreover, the TEM microscopic analysis proved that the correct collagen structure was preserved after the decellularization process. Based on the obtained results, we chose the most favorable variant in terms of quality and biology. The method we chose is an effective and safe method that gives a chance for the development of transplant and regenerative medicine.
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spelling pubmed-82676642021-07-10 Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM Klak, Marta Łojszczyk, Ilona Berman, Andrzej Tymicki, Grzegorz Adamiok-Ostrowska, Anna Sierakowski, Maciej Olkowski, Radosław Szczepankiewicz, Andrzej Antoni Kamiński, Artur Dobrzyń, Agnieszka Wszoła, Michał Int J Mol Sci Article Due to the limited number of organ donors, 3D printing of organs is a promising technique. Tissue engineering is increasingly using xenogeneic material for this purpose. This study was aimed at assessing the safety of decellularized porcine pancreas, together with the analysis of the risk of an undesirable immune response. We tested eight variants of the decellularization process. We determined the following impacts: rinsing agents (PBS/NH(3)·H(2)O), temperature conditions (4 °C/24 °C), and the grinding method of native material (ground/cut). To assess the quality of the extracellular matrix after the completed decellularization process, analyses of the following were performed: DNA concentration, fat content, microscopic evaluation, proteolysis, material cytotoxicity, and most importantly, the Triton X-100 content. Our analyses showed that we obtained a product with an extremely low detergent content with negligible residual DNA content. The obtained results confirmed the performed histological and immuno-fluorescence staining. Moreover, the TEM microscopic analysis proved that the correct collagen structure was preserved after the decellularization process. Based on the obtained results, we chose the most favorable variant in terms of quality and biology. The method we chose is an effective and safe method that gives a chance for the development of transplant and regenerative medicine. MDPI 2021-06-29 /pmc/articles/PMC8267664/ /pubmed/34209772 http://dx.doi.org/10.3390/ijms22137005 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Klak, Marta
Łojszczyk, Ilona
Berman, Andrzej
Tymicki, Grzegorz
Adamiok-Ostrowska, Anna
Sierakowski, Maciej
Olkowski, Radosław
Szczepankiewicz, Andrzej Antoni
Kamiński, Artur
Dobrzyń, Agnieszka
Wszoła, Michał
Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM
title Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM
title_full Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM
title_fullStr Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM
title_full_unstemmed Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM
title_short Impact of Porcine Pancreas Decellularization Conditions on the Quality of Obtained dECM
title_sort impact of porcine pancreas decellularization conditions on the quality of obtained decm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267664/
https://www.ncbi.nlm.nih.gov/pubmed/34209772
http://dx.doi.org/10.3390/ijms22137005
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