Cargando…

Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs

(1) Background: In the current context of the COVID-19 crisis, there is a need for fast, easy-to-use, and sensitive diagnostic tools in addition to molecular methods. We have therefore decided to evaluate the performance of newly available antigen detection kits in “real-life” laboratory conditions....

Descripción completa

Detalles Bibliográficos
Autores principales: Seynaeve, Ysaline, Heylen, Justine, Fontaine, Corentin, Maclot, François, Meex, Cécile, Diep, Anh Nguyet, Donneau, Anne-Françoise, Hayette, Marie-Pierre, Descy, Julie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267731/
https://www.ncbi.nlm.nih.gov/pubmed/34202731
http://dx.doi.org/10.3390/jcm10132774
_version_ 1783720207215230976
author Seynaeve, Ysaline
Heylen, Justine
Fontaine, Corentin
Maclot, François
Meex, Cécile
Diep, Anh Nguyet
Donneau, Anne-Françoise
Hayette, Marie-Pierre
Descy, Julie
author_facet Seynaeve, Ysaline
Heylen, Justine
Fontaine, Corentin
Maclot, François
Meex, Cécile
Diep, Anh Nguyet
Donneau, Anne-Françoise
Hayette, Marie-Pierre
Descy, Julie
author_sort Seynaeve, Ysaline
collection PubMed
description (1) Background: In the current context of the COVID-19 crisis, there is a need for fast, easy-to-use, and sensitive diagnostic tools in addition to molecular methods. We have therefore decided to evaluate the performance of newly available antigen detection kits in “real-life” laboratory conditions. (2) Methods: The sensitivity and specificity of two rapid diagnostic tests (RDT)—the COVID-19 Ag Respi-Strip from Coris Bioconcept, Belgium (CoRDT), and the coronavirus antigen rapid test cassette from Healgen Scientific, LLC, USA (HeRDT)—were evaluated on 193 nasopharyngeal samples using RT-PCR as the gold standard. (3) Results: The sensitivity obtained for HeRDT was 88% for all collected samples and 91.1% for samples with Ct ≤ 31. For the CoRDT test, the sensitivity obtained was 62% for all collected samples and 68.9% for samples with Ct ≤ 31. (4) Conclusions: Despite the excellent specificity obtained for both kits, the poor sensitivity of the CoRDT did not allow for its use in the rapid diagnosis of COVID-19. HeRDT satisfied the World Health Organization’s performance criteria for rapid antigen detection tests. Its high sensitivity, quick response, and ease of use allowed for the implementation of HeRDT at the laboratory of the University Hospital of Liège.
format Online
Article
Text
id pubmed-8267731
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82677312021-07-10 Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs Seynaeve, Ysaline Heylen, Justine Fontaine, Corentin Maclot, François Meex, Cécile Diep, Anh Nguyet Donneau, Anne-Françoise Hayette, Marie-Pierre Descy, Julie J Clin Med Article (1) Background: In the current context of the COVID-19 crisis, there is a need for fast, easy-to-use, and sensitive diagnostic tools in addition to molecular methods. We have therefore decided to evaluate the performance of newly available antigen detection kits in “real-life” laboratory conditions. (2) Methods: The sensitivity and specificity of two rapid diagnostic tests (RDT)—the COVID-19 Ag Respi-Strip from Coris Bioconcept, Belgium (CoRDT), and the coronavirus antigen rapid test cassette from Healgen Scientific, LLC, USA (HeRDT)—were evaluated on 193 nasopharyngeal samples using RT-PCR as the gold standard. (3) Results: The sensitivity obtained for HeRDT was 88% for all collected samples and 91.1% for samples with Ct ≤ 31. For the CoRDT test, the sensitivity obtained was 62% for all collected samples and 68.9% for samples with Ct ≤ 31. (4) Conclusions: Despite the excellent specificity obtained for both kits, the poor sensitivity of the CoRDT did not allow for its use in the rapid diagnosis of COVID-19. HeRDT satisfied the World Health Organization’s performance criteria for rapid antigen detection tests. Its high sensitivity, quick response, and ease of use allowed for the implementation of HeRDT at the laboratory of the University Hospital of Liège. MDPI 2021-06-24 /pmc/articles/PMC8267731/ /pubmed/34202731 http://dx.doi.org/10.3390/jcm10132774 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Seynaeve, Ysaline
Heylen, Justine
Fontaine, Corentin
Maclot, François
Meex, Cécile
Diep, Anh Nguyet
Donneau, Anne-Françoise
Hayette, Marie-Pierre
Descy, Julie
Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs
title Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs
title_full Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs
title_fullStr Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs
title_full_unstemmed Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs
title_short Evaluation of Two Rapid Antigenic Tests for the Detection of SARS-CoV-2 in Nasopharyngeal Swabs
title_sort evaluation of two rapid antigenic tests for the detection of sars-cov-2 in nasopharyngeal swabs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267731/
https://www.ncbi.nlm.nih.gov/pubmed/34202731
http://dx.doi.org/10.3390/jcm10132774
work_keys_str_mv AT seynaeveysaline evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT heylenjustine evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT fontainecorentin evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT maclotfrancois evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT meexcecile evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT diepanhnguyet evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT donneauannefrancoise evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT hayettemariepierre evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs
AT descyjulie evaluationoftworapidantigenictestsforthedetectionofsarscov2innasopharyngealswabs