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Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF

The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimpla...

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Autores principales: Pierzchała, Dorota, Liput, Kamila, Korwin-Kossakowska, Agnieszka, Ogłuszka, Magdalena, Poławska, Ewa, Nawrocka, Agata, Urbański, Paweł, Ciepłoch, Aleksandra, Juszczuk-Kubiak, Edyta, Lepczyński, Adam, Ślaska, Brygida, Kowal, Krzysztof, te Pas, Marinus F. W., Śmiech, Magdalena, Leszczyński, Paweł, Taniguchi, Hiroaki, Fraser, Leyland, Sobiech, Przemysław, Sachajko, Mateusz, Herudzinska, Magdalena, Pareek, Chandra S., Pierzchała, Mariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267828/
https://www.ncbi.nlm.nih.gov/pubmed/34201586
http://dx.doi.org/10.3390/ijms22136720
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author Pierzchała, Dorota
Liput, Kamila
Korwin-Kossakowska, Agnieszka
Ogłuszka, Magdalena
Poławska, Ewa
Nawrocka, Agata
Urbański, Paweł
Ciepłoch, Aleksandra
Juszczuk-Kubiak, Edyta
Lepczyński, Adam
Ślaska, Brygida
Kowal, Krzysztof
te Pas, Marinus F. W.
Śmiech, Magdalena
Leszczyński, Paweł
Taniguchi, Hiroaki
Fraser, Leyland
Sobiech, Przemysław
Sachajko, Mateusz
Herudzinska, Magdalena
Pareek, Chandra S.
Pierzchała, Mariusz
author_facet Pierzchała, Dorota
Liput, Kamila
Korwin-Kossakowska, Agnieszka
Ogłuszka, Magdalena
Poławska, Ewa
Nawrocka, Agata
Urbański, Paweł
Ciepłoch, Aleksandra
Juszczuk-Kubiak, Edyta
Lepczyński, Adam
Ślaska, Brygida
Kowal, Krzysztof
te Pas, Marinus F. W.
Śmiech, Magdalena
Leszczyński, Paweł
Taniguchi, Hiroaki
Fraser, Leyland
Sobiech, Przemysław
Sachajko, Mateusz
Herudzinska, Magdalena
Pareek, Chandra S.
Pierzchała, Mariusz
author_sort Pierzchała, Dorota
collection PubMed
description The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGO(TM) approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts.
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spelling pubmed-82678282021-07-10 Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF Pierzchała, Dorota Liput, Kamila Korwin-Kossakowska, Agnieszka Ogłuszka, Magdalena Poławska, Ewa Nawrocka, Agata Urbański, Paweł Ciepłoch, Aleksandra Juszczuk-Kubiak, Edyta Lepczyński, Adam Ślaska, Brygida Kowal, Krzysztof te Pas, Marinus F. W. Śmiech, Magdalena Leszczyński, Paweł Taniguchi, Hiroaki Fraser, Leyland Sobiech, Przemysław Sachajko, Mateusz Herudzinska, Magdalena Pareek, Chandra S. Pierzchała, Mariusz Int J Mol Sci Article The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGO(TM) approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts. MDPI 2021-06-23 /pmc/articles/PMC8267828/ /pubmed/34201586 http://dx.doi.org/10.3390/ijms22136720 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pierzchała, Dorota
Liput, Kamila
Korwin-Kossakowska, Agnieszka
Ogłuszka, Magdalena
Poławska, Ewa
Nawrocka, Agata
Urbański, Paweł
Ciepłoch, Aleksandra
Juszczuk-Kubiak, Edyta
Lepczyński, Adam
Ślaska, Brygida
Kowal, Krzysztof
te Pas, Marinus F. W.
Śmiech, Magdalena
Leszczyński, Paweł
Taniguchi, Hiroaki
Fraser, Leyland
Sobiech, Przemysław
Sachajko, Mateusz
Herudzinska, Magdalena
Pareek, Chandra S.
Pierzchała, Mariusz
Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
title Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
title_full Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
title_fullStr Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
title_full_unstemmed Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
title_short Molecular Characterisation of Uterine Endometrial Proteins during Early Stages of Pregnancy in Pigs by MALDI TOF/TOF
title_sort molecular characterisation of uterine endometrial proteins during early stages of pregnancy in pigs by maldi tof/tof
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8267828/
https://www.ncbi.nlm.nih.gov/pubmed/34201586
http://dx.doi.org/10.3390/ijms22136720
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