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The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology
Apolipoprotein E (ApoE) isoforms exert intricate effects on cellular physiology beyond lipid transport and metabolism. ApoEs influence the onset of Alzheimer’s disease (AD) in an isoform-dependent manner: ApoE4 increases AD risk, while ApoE2 decreases it. Previously we demonstrated that syndecans, a...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268055/ https://www.ncbi.nlm.nih.gov/pubmed/34209175 http://dx.doi.org/10.3390/ijms22137070 |
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author | Hudák, Anett Jósvay, Katalin Domonkos, Ildikó Letoha, Annamária Szilák, László Letoha, Tamás |
author_facet | Hudák, Anett Jósvay, Katalin Domonkos, Ildikó Letoha, Annamária Szilák, László Letoha, Tamás |
author_sort | Hudák, Anett |
collection | PubMed |
description | Apolipoprotein E (ApoE) isoforms exert intricate effects on cellular physiology beyond lipid transport and metabolism. ApoEs influence the onset of Alzheimer’s disease (AD) in an isoform-dependent manner: ApoE4 increases AD risk, while ApoE2 decreases it. Previously we demonstrated that syndecans, a transmembrane proteoglycan family with increased expression in AD, trigger the aggregation and modulate the cellular uptake of amyloid beta (Aβ). Utilizing our previously established syndecan-overexpressing cellular assays, we now explore how the interplay of ApoEs with syndecans contributes to key events, namely uptake and aggregation, in Aβ pathology. The interaction of ApoEs with syndecans indicates isoform-specific characteristics arising beyond the frequently studied ApoE–heparan sulfate interactions. Syndecans, and among them the neuronal syndecan-3, increased the cellular uptake of ApoEs, especially ApoE2 and ApoE3, while ApoEs exerted opposing effects on syndecan-3-mediated Aβ uptake and aggregation. ApoE2 increased the cellular internalization of monomeric Aβ, hence preventing its extracellular aggregation, while ApoE4 decreased it, thus helping the buildup of extracellular plaques. The contrary effects of ApoE2 and ApoE4 remained once Aβ aggregated: while ApoE2 reduced the uptake of Aβ aggregates, ApoE4 facilitated it. Fibrillation studies also revealed ApoE4′s tendency to form fibrillar aggregates. Our results uncover yet unknown details of ApoE cellular biology and deepen our molecular understanding of the ApoE-dependent mechanism of Aβ pathology. |
format | Online Article Text |
id | pubmed-8268055 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82680552021-07-10 The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology Hudák, Anett Jósvay, Katalin Domonkos, Ildikó Letoha, Annamária Szilák, László Letoha, Tamás Int J Mol Sci Article Apolipoprotein E (ApoE) isoforms exert intricate effects on cellular physiology beyond lipid transport and metabolism. ApoEs influence the onset of Alzheimer’s disease (AD) in an isoform-dependent manner: ApoE4 increases AD risk, while ApoE2 decreases it. Previously we demonstrated that syndecans, a transmembrane proteoglycan family with increased expression in AD, trigger the aggregation and modulate the cellular uptake of amyloid beta (Aβ). Utilizing our previously established syndecan-overexpressing cellular assays, we now explore how the interplay of ApoEs with syndecans contributes to key events, namely uptake and aggregation, in Aβ pathology. The interaction of ApoEs with syndecans indicates isoform-specific characteristics arising beyond the frequently studied ApoE–heparan sulfate interactions. Syndecans, and among them the neuronal syndecan-3, increased the cellular uptake of ApoEs, especially ApoE2 and ApoE3, while ApoEs exerted opposing effects on syndecan-3-mediated Aβ uptake and aggregation. ApoE2 increased the cellular internalization of monomeric Aβ, hence preventing its extracellular aggregation, while ApoE4 decreased it, thus helping the buildup of extracellular plaques. The contrary effects of ApoE2 and ApoE4 remained once Aβ aggregated: while ApoE2 reduced the uptake of Aβ aggregates, ApoE4 facilitated it. Fibrillation studies also revealed ApoE4′s tendency to form fibrillar aggregates. Our results uncover yet unknown details of ApoE cellular biology and deepen our molecular understanding of the ApoE-dependent mechanism of Aβ pathology. MDPI 2021-06-30 /pmc/articles/PMC8268055/ /pubmed/34209175 http://dx.doi.org/10.3390/ijms22137070 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hudák, Anett Jósvay, Katalin Domonkos, Ildikó Letoha, Annamária Szilák, László Letoha, Tamás The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology |
title | The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology |
title_full | The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology |
title_fullStr | The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology |
title_full_unstemmed | The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology |
title_short | The Interplay of Apoes with Syndecans in Influencing Key Cellular Events of Amyloid Pathology |
title_sort | interplay of apoes with syndecans in influencing key cellular events of amyloid pathology |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268055/ https://www.ncbi.nlm.nih.gov/pubmed/34209175 http://dx.doi.org/10.3390/ijms22137070 |
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