Cargando…
Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity
Secreted frizzled-related protein 5 (SFRP5), an antagonist of the noncanonical WNT pathway, has a controversial role in liver disease. The aim of this study was to analyze the role of SFRP5 and the noncanonical WNT pathway in nonalcoholic fatty liver disease (NAFLD). Plasma SFRP5 levels were determi...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268103/ https://www.ncbi.nlm.nih.gov/pubmed/34198988 http://dx.doi.org/10.3390/ijms22136895 |
_version_ | 1783720283479212032 |
---|---|
author | Bertran, Laia Portillo-Carrasquer, Marta Aguilar, Carmen Porras, José Antonio Riesco, David Martínez, Salomé Vives, Margarita Sabench, Fàtima Gonzalez, Eva Del Castillo, Daniel Richart, Cristóbal Auguet, Teresa |
author_facet | Bertran, Laia Portillo-Carrasquer, Marta Aguilar, Carmen Porras, José Antonio Riesco, David Martínez, Salomé Vives, Margarita Sabench, Fàtima Gonzalez, Eva Del Castillo, Daniel Richart, Cristóbal Auguet, Teresa |
author_sort | Bertran, Laia |
collection | PubMed |
description | Secreted frizzled-related protein 5 (SFRP5), an antagonist of the noncanonical WNT pathway, has a controversial role in liver disease. The aim of this study was to analyze the role of SFRP5 and the noncanonical WNT pathway in nonalcoholic fatty liver disease (NAFLD). Plasma SFRP5 levels were determined by ELISA in women with normal weight (NW; n = 20) and morbid obesity (MO; n = 69). Women with MO were subclassified according to hepatic histology into normal liver (NL; n = 28), NAFLD (n = 41) (simple steatosis (SS; n = 24), and nonalcoholic steatohepatitis (NASH; n = 17)). We used RT-qPCR to evaluate the hepatic mRNA expression of SFRP5, WNT5A, and JNK in women with MO. SFRP5 levels were lower in NW than in MO patients who underwent a very low-calorie diet before surgery. Hepatic SFRP5 mRNA expression was higher in SS than in NL or NASH; additionally, patients with hepatic inflammation or ballooning presented lower SFRP5 abundance. WNT5A and JNK expression was enhanced in NAFLD compared with NL. In conclusion, circulating SFRP5 levels depend on the diet, and hepatic SFRP5 seems to have a protective role in the first steps of NAFLD; however, SFRP5 could be deregulated in an advanced stage while WNT5A and JNK are activated, promoting liver damage. |
format | Online Article Text |
id | pubmed-8268103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82681032021-07-10 Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity Bertran, Laia Portillo-Carrasquer, Marta Aguilar, Carmen Porras, José Antonio Riesco, David Martínez, Salomé Vives, Margarita Sabench, Fàtima Gonzalez, Eva Del Castillo, Daniel Richart, Cristóbal Auguet, Teresa Int J Mol Sci Article Secreted frizzled-related protein 5 (SFRP5), an antagonist of the noncanonical WNT pathway, has a controversial role in liver disease. The aim of this study was to analyze the role of SFRP5 and the noncanonical WNT pathway in nonalcoholic fatty liver disease (NAFLD). Plasma SFRP5 levels were determined by ELISA in women with normal weight (NW; n = 20) and morbid obesity (MO; n = 69). Women with MO were subclassified according to hepatic histology into normal liver (NL; n = 28), NAFLD (n = 41) (simple steatosis (SS; n = 24), and nonalcoholic steatohepatitis (NASH; n = 17)). We used RT-qPCR to evaluate the hepatic mRNA expression of SFRP5, WNT5A, and JNK in women with MO. SFRP5 levels were lower in NW than in MO patients who underwent a very low-calorie diet before surgery. Hepatic SFRP5 mRNA expression was higher in SS than in NL or NASH; additionally, patients with hepatic inflammation or ballooning presented lower SFRP5 abundance. WNT5A and JNK expression was enhanced in NAFLD compared with NL. In conclusion, circulating SFRP5 levels depend on the diet, and hepatic SFRP5 seems to have a protective role in the first steps of NAFLD; however, SFRP5 could be deregulated in an advanced stage while WNT5A and JNK are activated, promoting liver damage. MDPI 2021-06-27 /pmc/articles/PMC8268103/ /pubmed/34198988 http://dx.doi.org/10.3390/ijms22136895 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bertran, Laia Portillo-Carrasquer, Marta Aguilar, Carmen Porras, José Antonio Riesco, David Martínez, Salomé Vives, Margarita Sabench, Fàtima Gonzalez, Eva Del Castillo, Daniel Richart, Cristóbal Auguet, Teresa Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity |
title | Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity |
title_full | Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity |
title_fullStr | Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity |
title_full_unstemmed | Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity |
title_short | Deregulation of Secreted Frizzled-Related Protein 5 in Nonalcoholic Fatty Liver Disease Associated with Obesity |
title_sort | deregulation of secreted frizzled-related protein 5 in nonalcoholic fatty liver disease associated with obesity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268103/ https://www.ncbi.nlm.nih.gov/pubmed/34198988 http://dx.doi.org/10.3390/ijms22136895 |
work_keys_str_mv | AT bertranlaia deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT portillocarrasquermarta deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT aguilarcarmen deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT porrasjoseantonio deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT riescodavid deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT martinezsalome deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT vivesmargarita deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT sabenchfatima deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT gonzalezeva deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT delcastillodaniel deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT richartcristobal deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity AT auguetteresa deregulationofsecretedfrizzledrelatedprotein5innonalcoholicfattyliverdiseaseassociatedwithobesity |