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SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data
We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost 100-fold differences between laboratories when blind-testing 15 plasma samples. Calibration of titres in relation to the referen...
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
European Centre for Disease Prevention and Control (ECDC)
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268650/ https://www.ncbi.nlm.nih.gov/pubmed/34240697 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.27.2100568 |
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| author | Nguyen, Dung Simmonds, Peter Steenhuis, Maurice Wouters, Elise Desmecht, Daniel Garigliany, Mutien Romano, Marta Barbezange, Cyril Maes, Piet Van Holm, Bram Mendoza, Joaquín Oyonarte, Salvador Fomsgaard, Anders Lassaunière, Ria Zusinaite, Eva Resman Rus, Katarina Avšič-Županc, Tatjana Reimerink, Johan HJ Brouwer, Fiona Hoogerwerf, Marieke Reusken, Chantal BEM Grodeland, Gunnveig Le Cam, Sophie Gallian, Pierre Amroun, Abdennour Brisbarre, Nadège Martinaud, Christophe Leparc Goffart, Isabelle Schrezenmeier, Hubert Feys, Hendrik B van der Schoot, C Ellen Harvala, Heli |
| author_facet | Nguyen, Dung Simmonds, Peter Steenhuis, Maurice Wouters, Elise Desmecht, Daniel Garigliany, Mutien Romano, Marta Barbezange, Cyril Maes, Piet Van Holm, Bram Mendoza, Joaquín Oyonarte, Salvador Fomsgaard, Anders Lassaunière, Ria Zusinaite, Eva Resman Rus, Katarina Avšič-Županc, Tatjana Reimerink, Johan HJ Brouwer, Fiona Hoogerwerf, Marieke Reusken, Chantal BEM Grodeland, Gunnveig Le Cam, Sophie Gallian, Pierre Amroun, Abdennour Brisbarre, Nadège Martinaud, Christophe Leparc Goffart, Isabelle Schrezenmeier, Hubert Feys, Hendrik B van der Schoot, C Ellen Harvala, Heli |
| author_sort | Nguyen, Dung |
| collection | PubMed |
| description | We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost 100-fold differences between laboratories when blind-testing 15 plasma samples. Calibration of titres in relation to the reference reagent and standard curve obtained by testing a dilution series reduced the inter-laboratory variability ca 10-fold. The harmonisation of neutralising antibody quantification is a vital step towards determining the protective and therapeutic levels of neutralising antibodies. |
| format | Online Article Text |
| id | pubmed-8268650 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | European Centre for Disease Prevention and Control (ECDC) |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-82686502021-07-16 SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data Nguyen, Dung Simmonds, Peter Steenhuis, Maurice Wouters, Elise Desmecht, Daniel Garigliany, Mutien Romano, Marta Barbezange, Cyril Maes, Piet Van Holm, Bram Mendoza, Joaquín Oyonarte, Salvador Fomsgaard, Anders Lassaunière, Ria Zusinaite, Eva Resman Rus, Katarina Avšič-Županc, Tatjana Reimerink, Johan HJ Brouwer, Fiona Hoogerwerf, Marieke Reusken, Chantal BEM Grodeland, Gunnveig Le Cam, Sophie Gallian, Pierre Amroun, Abdennour Brisbarre, Nadège Martinaud, Christophe Leparc Goffart, Isabelle Schrezenmeier, Hubert Feys, Hendrik B van der Schoot, C Ellen Harvala, Heli Euro Surveill Rapid Communication We compared the performance of SARS-CoV-2 neutralising antibody testing between 12 European laboratories involved in convalescent plasma trials. Raw titres differed almost 100-fold differences between laboratories when blind-testing 15 plasma samples. Calibration of titres in relation to the reference reagent and standard curve obtained by testing a dilution series reduced the inter-laboratory variability ca 10-fold. The harmonisation of neutralising antibody quantification is a vital step towards determining the protective and therapeutic levels of neutralising antibodies. European Centre for Disease Prevention and Control (ECDC) 2021-07-08 /pmc/articles/PMC8268650/ /pubmed/34240697 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.27.2100568 Text en This article is copyright of the authors or their affiliated institutions, 2021. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made. |
| spellingShingle | Rapid Communication Nguyen, Dung Simmonds, Peter Steenhuis, Maurice Wouters, Elise Desmecht, Daniel Garigliany, Mutien Romano, Marta Barbezange, Cyril Maes, Piet Van Holm, Bram Mendoza, Joaquín Oyonarte, Salvador Fomsgaard, Anders Lassaunière, Ria Zusinaite, Eva Resman Rus, Katarina Avšič-Županc, Tatjana Reimerink, Johan HJ Brouwer, Fiona Hoogerwerf, Marieke Reusken, Chantal BEM Grodeland, Gunnveig Le Cam, Sophie Gallian, Pierre Amroun, Abdennour Brisbarre, Nadège Martinaud, Christophe Leparc Goffart, Isabelle Schrezenmeier, Hubert Feys, Hendrik B van der Schoot, C Ellen Harvala, Heli SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data |
| title | SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data |
| title_full | SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data |
| title_fullStr | SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data |
| title_full_unstemmed | SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data |
| title_short | SARS-CoV-2 neutralising antibody testing in Europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data |
| title_sort | sars-cov-2 neutralising antibody testing in europe: towards harmonisation of neutralising antibody titres for better use of convalescent plasma and comparability of trial data |
| topic | Rapid Communication |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268650/ https://www.ncbi.nlm.nih.gov/pubmed/34240697 http://dx.doi.org/10.2807/1560-7917.ES.2021.26.27.2100568 |
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