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Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma

Background: Thyroid carcinoma (TC) is the most common endocrine system malignancy, and papillary thyroid carcinoma (PTC) accounts for >80% of all TC cases. Nevertheless, PTC pathogenesis is still not fully understood. The aim of the study was to elucidate the role of the FRMD5 protein in the regu...

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Autores principales: Gaweł, Agata M., Ratajczak, Maciej, Gajda, Ewa, Grzanka, Małgorzata, Paziewska, Agnieszka, Cieślicka, Marta, Kulecka, Maria, Oczko-Wojciechowska, Małgorzata, Godlewska, Marlena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268710/
https://www.ncbi.nlm.nih.gov/pubmed/34201607
http://dx.doi.org/10.3390/ijms22136726
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author Gaweł, Agata M.
Ratajczak, Maciej
Gajda, Ewa
Grzanka, Małgorzata
Paziewska, Agnieszka
Cieślicka, Marta
Kulecka, Maria
Oczko-Wojciechowska, Małgorzata
Godlewska, Marlena
author_facet Gaweł, Agata M.
Ratajczak, Maciej
Gajda, Ewa
Grzanka, Małgorzata
Paziewska, Agnieszka
Cieślicka, Marta
Kulecka, Maria
Oczko-Wojciechowska, Małgorzata
Godlewska, Marlena
author_sort Gaweł, Agata M.
collection PubMed
description Background: Thyroid carcinoma (TC) is the most common endocrine system malignancy, and papillary thyroid carcinoma (PTC) accounts for >80% of all TC cases. Nevertheless, PTC pathogenesis is still not fully understood. The aim of the study was to elucidate the role of the FRMD5 protein in the regulation of biological pathways associated with the development of PTC. We imply that the presence of certain genetic aberrations (e.g., BRAF V600E mutation) is associated with the activity of FRMD5. Methods: The studies were conducted on TPC1 and BCPAP (BRAF V600E) model PTC-derived cells. Transfection with siRNA was used to deplete the expression of FRMD5. The mRNA expression and protein yield were evaluated using RT-qPCR and Western blot techniques. Proliferation, migration, invasiveness, adhesion, spheroid formation, and survival tests were performed. RNA sequencing and phospho-kinase proteome profiling were used to assess signaling pathways associated with the FRMD5 expressional status. Results: The obtained data indicate that the expression of FRMD5 is significantly enhanced in BRAF V600E tumor specimens and cells. It was observed that a drop in intracellular yield of FRMD5 results in significant alternations in the migration, invasiveness, adhesion, and spheroid formation potential of PTC-derived cells. Importantly, significant divergences in the effect of FRMD5 depletion in both BRAF-wt and BRAF-mutated PTC cells were observed. It was also found that knockdown of FRMD5 significantly alters the expression of multidrug resistant genes. Conclusions: This is the first report highlighting the importance of the FRMD5 protein in the biology of PTCs. The results suggest that the FRMD5 protein can play an important role in controlling the metastatic potential and multidrug resistance of thyroid tumor cells.
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spelling pubmed-82687102021-07-10 Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma Gaweł, Agata M. Ratajczak, Maciej Gajda, Ewa Grzanka, Małgorzata Paziewska, Agnieszka Cieślicka, Marta Kulecka, Maria Oczko-Wojciechowska, Małgorzata Godlewska, Marlena Int J Mol Sci Article Background: Thyroid carcinoma (TC) is the most common endocrine system malignancy, and papillary thyroid carcinoma (PTC) accounts for >80% of all TC cases. Nevertheless, PTC pathogenesis is still not fully understood. The aim of the study was to elucidate the role of the FRMD5 protein in the regulation of biological pathways associated with the development of PTC. We imply that the presence of certain genetic aberrations (e.g., BRAF V600E mutation) is associated with the activity of FRMD5. Methods: The studies were conducted on TPC1 and BCPAP (BRAF V600E) model PTC-derived cells. Transfection with siRNA was used to deplete the expression of FRMD5. The mRNA expression and protein yield were evaluated using RT-qPCR and Western blot techniques. Proliferation, migration, invasiveness, adhesion, spheroid formation, and survival tests were performed. RNA sequencing and phospho-kinase proteome profiling were used to assess signaling pathways associated with the FRMD5 expressional status. Results: The obtained data indicate that the expression of FRMD5 is significantly enhanced in BRAF V600E tumor specimens and cells. It was observed that a drop in intracellular yield of FRMD5 results in significant alternations in the migration, invasiveness, adhesion, and spheroid formation potential of PTC-derived cells. Importantly, significant divergences in the effect of FRMD5 depletion in both BRAF-wt and BRAF-mutated PTC cells were observed. It was also found that knockdown of FRMD5 significantly alters the expression of multidrug resistant genes. Conclusions: This is the first report highlighting the importance of the FRMD5 protein in the biology of PTCs. The results suggest that the FRMD5 protein can play an important role in controlling the metastatic potential and multidrug resistance of thyroid tumor cells. MDPI 2021-06-23 /pmc/articles/PMC8268710/ /pubmed/34201607 http://dx.doi.org/10.3390/ijms22136726 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gaweł, Agata M.
Ratajczak, Maciej
Gajda, Ewa
Grzanka, Małgorzata
Paziewska, Agnieszka
Cieślicka, Marta
Kulecka, Maria
Oczko-Wojciechowska, Małgorzata
Godlewska, Marlena
Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma
title Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma
title_full Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma
title_fullStr Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma
title_full_unstemmed Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma
title_short Analysis of the Role of FRMD5 in the Biology of Papillary Thyroid Carcinoma
title_sort analysis of the role of frmd5 in the biology of papillary thyroid carcinoma
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268710/
https://www.ncbi.nlm.nih.gov/pubmed/34201607
http://dx.doi.org/10.3390/ijms22136726
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