Cargando…
New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
Cytocentrifugation is a common technique for the capture of cells on microscopic slides. It usually requires a special cytocentrifuge or cytorotor and cassettes. In the study presented here, we tested the new concept of cytocentrifugation based on the threaded connection of the lid and the sample ho...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268716/ https://www.ncbi.nlm.nih.gov/pubmed/34281153 http://dx.doi.org/10.3390/ijms22137098 |
_version_ | 1783720419952427008 |
---|---|
author | Ligasová, Anna Koberna, Karel |
author_facet | Ligasová, Anna Koberna, Karel |
author_sort | Ligasová, Anna |
collection | PubMed |
description | Cytocentrifugation is a common technique for the capture of cells on microscopic slides. It usually requires a special cytocentrifuge or cytorotor and cassettes. In the study presented here, we tested the new concept of cytocentrifugation based on the threaded connection of the lid and the sample holder to ensure an adjustable flow of solutions through the filters and the collection of the filtered solutions in the reservoir during centrifugation. To test this concept, we developed a device for the preparation of cell samples on circular coverslips. The device was tested for the capture and sample processing of both eukaryotic and prokaryotic cells, cell nuclei, and mitochondria for microscopy analysis including image cytometry. Moreover, an efficient procedure was developed for capturing formaldehyde-fixed cells on non-treated coverslips without cell drying. The results showed that the tested arrangement enables the effective capture and processing of all of the tested samples and the developed device represents an inexpensive alternative to common cytocentrifuges, as only the paper filter is consumed during sample processing, and no special centrifuge, cytorotor, or cassette is necessary. As no additional system of solution removal is required during sample staining, the tested concept also facilitates the eventual automation of the staining procedure. |
format | Online Article Text |
id | pubmed-8268716 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82687162021-07-10 New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis Ligasová, Anna Koberna, Karel Int J Mol Sci Article Cytocentrifugation is a common technique for the capture of cells on microscopic slides. It usually requires a special cytocentrifuge or cytorotor and cassettes. In the study presented here, we tested the new concept of cytocentrifugation based on the threaded connection of the lid and the sample holder to ensure an adjustable flow of solutions through the filters and the collection of the filtered solutions in the reservoir during centrifugation. To test this concept, we developed a device for the preparation of cell samples on circular coverslips. The device was tested for the capture and sample processing of both eukaryotic and prokaryotic cells, cell nuclei, and mitochondria for microscopy analysis including image cytometry. Moreover, an efficient procedure was developed for capturing formaldehyde-fixed cells on non-treated coverslips without cell drying. The results showed that the tested arrangement enables the effective capture and processing of all of the tested samples and the developed device represents an inexpensive alternative to common cytocentrifuges, as only the paper filter is consumed during sample processing, and no special centrifuge, cytorotor, or cassette is necessary. As no additional system of solution removal is required during sample staining, the tested concept also facilitates the eventual automation of the staining procedure. MDPI 2021-07-01 /pmc/articles/PMC8268716/ /pubmed/34281153 http://dx.doi.org/10.3390/ijms22137098 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ligasová, Anna Koberna, Karel New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis |
title | New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis |
title_full | New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis |
title_fullStr | New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis |
title_full_unstemmed | New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis |
title_short | New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis |
title_sort | new concept and apparatus for cytocentrifugation and cell processing for microscopy analysis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268716/ https://www.ncbi.nlm.nih.gov/pubmed/34281153 http://dx.doi.org/10.3390/ijms22137098 |
work_keys_str_mv | AT ligasovaanna newconceptandapparatusforcytocentrifugationandcellprocessingformicroscopyanalysis AT kobernakarel newconceptandapparatusforcytocentrifugationandcellprocessingformicroscopyanalysis |