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New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis

Cytocentrifugation is a common technique for the capture of cells on microscopic slides. It usually requires a special cytocentrifuge or cytorotor and cassettes. In the study presented here, we tested the new concept of cytocentrifugation based on the threaded connection of the lid and the sample ho...

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Autores principales: Ligasová, Anna, Koberna, Karel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268716/
https://www.ncbi.nlm.nih.gov/pubmed/34281153
http://dx.doi.org/10.3390/ijms22137098
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author Ligasová, Anna
Koberna, Karel
author_facet Ligasová, Anna
Koberna, Karel
author_sort Ligasová, Anna
collection PubMed
description Cytocentrifugation is a common technique for the capture of cells on microscopic slides. It usually requires a special cytocentrifuge or cytorotor and cassettes. In the study presented here, we tested the new concept of cytocentrifugation based on the threaded connection of the lid and the sample holder to ensure an adjustable flow of solutions through the filters and the collection of the filtered solutions in the reservoir during centrifugation. To test this concept, we developed a device for the preparation of cell samples on circular coverslips. The device was tested for the capture and sample processing of both eukaryotic and prokaryotic cells, cell nuclei, and mitochondria for microscopy analysis including image cytometry. Moreover, an efficient procedure was developed for capturing formaldehyde-fixed cells on non-treated coverslips without cell drying. The results showed that the tested arrangement enables the effective capture and processing of all of the tested samples and the developed device represents an inexpensive alternative to common cytocentrifuges, as only the paper filter is consumed during sample processing, and no special centrifuge, cytorotor, or cassette is necessary. As no additional system of solution removal is required during sample staining, the tested concept also facilitates the eventual automation of the staining procedure.
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spelling pubmed-82687162021-07-10 New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis Ligasová, Anna Koberna, Karel Int J Mol Sci Article Cytocentrifugation is a common technique for the capture of cells on microscopic slides. It usually requires a special cytocentrifuge or cytorotor and cassettes. In the study presented here, we tested the new concept of cytocentrifugation based on the threaded connection of the lid and the sample holder to ensure an adjustable flow of solutions through the filters and the collection of the filtered solutions in the reservoir during centrifugation. To test this concept, we developed a device for the preparation of cell samples on circular coverslips. The device was tested for the capture and sample processing of both eukaryotic and prokaryotic cells, cell nuclei, and mitochondria for microscopy analysis including image cytometry. Moreover, an efficient procedure was developed for capturing formaldehyde-fixed cells on non-treated coverslips without cell drying. The results showed that the tested arrangement enables the effective capture and processing of all of the tested samples and the developed device represents an inexpensive alternative to common cytocentrifuges, as only the paper filter is consumed during sample processing, and no special centrifuge, cytorotor, or cassette is necessary. As no additional system of solution removal is required during sample staining, the tested concept also facilitates the eventual automation of the staining procedure. MDPI 2021-07-01 /pmc/articles/PMC8268716/ /pubmed/34281153 http://dx.doi.org/10.3390/ijms22137098 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ligasová, Anna
Koberna, Karel
New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
title New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
title_full New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
title_fullStr New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
title_full_unstemmed New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
title_short New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis
title_sort new concept and apparatus for cytocentrifugation and cell processing for microscopy analysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268716/
https://www.ncbi.nlm.nih.gov/pubmed/34281153
http://dx.doi.org/10.3390/ijms22137098
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