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Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis

miRNAs are involved in various biological processes, including adaptive responses to abiotic stress. To understand the role of miRNAs in the response to ABA, ABA-responsive miRNAs were identified by small RNA sequencing in wild-type Arabidopsis, as well as in abi1td, mkkk17, and mkkk18 mutants. We i...

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Autores principales: Mehdi, Syed Muhammad Muntazir, Krishnamoorthy, Sivakumar, Szczesniak, Michal Wojciech, Ludwików, Agnieszka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268864/
https://www.ncbi.nlm.nih.gov/pubmed/34281207
http://dx.doi.org/10.3390/ijms22137153
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author Mehdi, Syed Muhammad Muntazir
Krishnamoorthy, Sivakumar
Szczesniak, Michal Wojciech
Ludwików, Agnieszka
author_facet Mehdi, Syed Muhammad Muntazir
Krishnamoorthy, Sivakumar
Szczesniak, Michal Wojciech
Ludwików, Agnieszka
author_sort Mehdi, Syed Muhammad Muntazir
collection PubMed
description miRNAs are involved in various biological processes, including adaptive responses to abiotic stress. To understand the role of miRNAs in the response to ABA, ABA-responsive miRNAs were identified by small RNA sequencing in wild-type Arabidopsis, as well as in abi1td, mkkk17, and mkkk18 mutants. We identified 10 novel miRNAs in WT after ABA treatment, while in abi1td, mkkk17, and mkkk18 mutants, three, seven, and nine known miRNAs, respectively, were differentially expressed after ABA treatment. One novel miRNA (miRn-8) was differentially expressed in the mkkk17 mutant. Potential target genes of the miRNA panel were identified using psRNATarget. Sequencing results were validated by quantitative RT-PCR of several known and novel miRNAs in all genotypes. Of the predicted targets of novel miRNAs, seven target genes of six novel miRNAs were further validated by 5′ RLM-RACE. Gene ontology analyses showed the potential target genes of ABA-responsive known and novel miRNAs to be involved in diverse cellular processes in plants, including development and stomatal movement. These outcomes suggest that a number of the identified miRNAs have crucial roles in plant responses to environmental stress, as well as in plant development, and might have common regulatory roles in the core ABA signaling pathway.
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spelling pubmed-82688642021-07-10 Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis Mehdi, Syed Muhammad Muntazir Krishnamoorthy, Sivakumar Szczesniak, Michal Wojciech Ludwików, Agnieszka Int J Mol Sci Article miRNAs are involved in various biological processes, including adaptive responses to abiotic stress. To understand the role of miRNAs in the response to ABA, ABA-responsive miRNAs were identified by small RNA sequencing in wild-type Arabidopsis, as well as in abi1td, mkkk17, and mkkk18 mutants. We identified 10 novel miRNAs in WT after ABA treatment, while in abi1td, mkkk17, and mkkk18 mutants, three, seven, and nine known miRNAs, respectively, were differentially expressed after ABA treatment. One novel miRNA (miRn-8) was differentially expressed in the mkkk17 mutant. Potential target genes of the miRNA panel were identified using psRNATarget. Sequencing results were validated by quantitative RT-PCR of several known and novel miRNAs in all genotypes. Of the predicted targets of novel miRNAs, seven target genes of six novel miRNAs were further validated by 5′ RLM-RACE. Gene ontology analyses showed the potential target genes of ABA-responsive known and novel miRNAs to be involved in diverse cellular processes in plants, including development and stomatal movement. These outcomes suggest that a number of the identified miRNAs have crucial roles in plant responses to environmental stress, as well as in plant development, and might have common regulatory roles in the core ABA signaling pathway. MDPI 2021-07-01 /pmc/articles/PMC8268864/ /pubmed/34281207 http://dx.doi.org/10.3390/ijms22137153 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mehdi, Syed Muhammad Muntazir
Krishnamoorthy, Sivakumar
Szczesniak, Michal Wojciech
Ludwików, Agnieszka
Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis
title Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis
title_full Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis
title_fullStr Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis
title_full_unstemmed Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis
title_short Identification of Novel miRNAs and Their Target Genes in the Response to Abscisic Acid in Arabidopsis
title_sort identification of novel mirnas and their target genes in the response to abscisic acid in arabidopsis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8268864/
https://www.ncbi.nlm.nih.gov/pubmed/34281207
http://dx.doi.org/10.3390/ijms22137153
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