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Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene

The death of photoreceptor cells is induced by continuous light exposure. However, it is unclear whether light damage was induced in retinal ganglion cells with photosensitivity by transduction of optogenetic genes. In this study, we evaluated the phototoxicities of continuous light exposure on reti...

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Autores principales: Tabata, Kitako, Sugano, Eriko, Hatakeyama, Akito, Watanabe, Yoshito, Suzuki, Tomoya, Ozaki, Taku, Fukuda, Tomokazu, Tomita, Hiroshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269149/
https://www.ncbi.nlm.nih.gov/pubmed/34201658
http://dx.doi.org/10.3390/ijms22136732
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author Tabata, Kitako
Sugano, Eriko
Hatakeyama, Akito
Watanabe, Yoshito
Suzuki, Tomoya
Ozaki, Taku
Fukuda, Tomokazu
Tomita, Hiroshi
author_facet Tabata, Kitako
Sugano, Eriko
Hatakeyama, Akito
Watanabe, Yoshito
Suzuki, Tomoya
Ozaki, Taku
Fukuda, Tomokazu
Tomita, Hiroshi
author_sort Tabata, Kitako
collection PubMed
description The death of photoreceptor cells is induced by continuous light exposure. However, it is unclear whether light damage was induced in retinal ganglion cells with photosensitivity by transduction of optogenetic genes. In this study, we evaluated the phototoxicities of continuous light exposure on retinal ganglion cells after transduction of the optogenetic gene mVChR1 using an adeno-associated virus vector. Rats were exposed to continuous light for a week, and visually evoked potentials (VEPs) were recorded. The intensities of continuous light (500, 1000, 3000, and 5000 lx) increased substantially after VEP recordings. After the final recording of VEPs, retinal ganglion cells (RGCs) were retrogradely labeled with a fluorescein tracer, FluoroGold, and the number of retinal ganglion cells was counted under a fluorescent microscope. There was no significant reduction in the amplitudes of VEPs and the number of RGCs after exposure to any light intensity. These results indicated that RGCs were photosensitive after the transduction of optogenetic genes and did not induce any phototoxicity by continuous light exposure.
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spelling pubmed-82691492021-07-10 Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene Tabata, Kitako Sugano, Eriko Hatakeyama, Akito Watanabe, Yoshito Suzuki, Tomoya Ozaki, Taku Fukuda, Tomokazu Tomita, Hiroshi Int J Mol Sci Article The death of photoreceptor cells is induced by continuous light exposure. However, it is unclear whether light damage was induced in retinal ganglion cells with photosensitivity by transduction of optogenetic genes. In this study, we evaluated the phototoxicities of continuous light exposure on retinal ganglion cells after transduction of the optogenetic gene mVChR1 using an adeno-associated virus vector. Rats were exposed to continuous light for a week, and visually evoked potentials (VEPs) were recorded. The intensities of continuous light (500, 1000, 3000, and 5000 lx) increased substantially after VEP recordings. After the final recording of VEPs, retinal ganglion cells (RGCs) were retrogradely labeled with a fluorescein tracer, FluoroGold, and the number of retinal ganglion cells was counted under a fluorescent microscope. There was no significant reduction in the amplitudes of VEPs and the number of RGCs after exposure to any light intensity. These results indicated that RGCs were photosensitive after the transduction of optogenetic genes and did not induce any phototoxicity by continuous light exposure. MDPI 2021-06-23 /pmc/articles/PMC8269149/ /pubmed/34201658 http://dx.doi.org/10.3390/ijms22136732 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tabata, Kitako
Sugano, Eriko
Hatakeyama, Akito
Watanabe, Yoshito
Suzuki, Tomoya
Ozaki, Taku
Fukuda, Tomokazu
Tomita, Hiroshi
Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene
title Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene
title_full Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene
title_fullStr Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene
title_full_unstemmed Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene
title_short Phototoxicities Caused by Continuous Light Exposure Were Not Induced in Retinal Ganglion Cells Transduced by an Optogenetic Gene
title_sort phototoxicities caused by continuous light exposure were not induced in retinal ganglion cells transduced by an optogenetic gene
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269149/
https://www.ncbi.nlm.nih.gov/pubmed/34201658
http://dx.doi.org/10.3390/ijms22136732
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