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Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation

Bacterial meningitis shows a higher incidence in children than adults, but signs may be scarce. Although some pathogenic microorganisms of meningitis from cerebrospinal fluid (CSF) have been reported, the signature of the representative microbiota in CSF and blood samples from patients remains incom...

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Autores principales: Liao, Huiping, Zhang, Yuchao, Guo, Wei, Wang, Xi, Wang, Hailong, Ye, Haocheng, Wu, Kai, Zhang, Yu-Hang, Guo, Lingyun, Zhu, Yufei, Guo, Yongli, Hu, Landian, Liu, Gang, Kong, Xiangyin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269202/
https://www.ncbi.nlm.nih.gov/pubmed/34100633
http://dx.doi.org/10.1128/mSystems.00049-21
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author Liao, Huiping
Zhang, Yuchao
Guo, Wei
Wang, Xi
Wang, Hailong
Ye, Haocheng
Wu, Kai
Zhang, Yu-Hang
Guo, Lingyun
Zhu, Yufei
Guo, Yongli
Hu, Landian
Liu, Gang
Kong, Xiangyin
author_facet Liao, Huiping
Zhang, Yuchao
Guo, Wei
Wang, Xi
Wang, Hailong
Ye, Haocheng
Wu, Kai
Zhang, Yu-Hang
Guo, Lingyun
Zhu, Yufei
Guo, Yongli
Hu, Landian
Liu, Gang
Kong, Xiangyin
author_sort Liao, Huiping
collection PubMed
description Bacterial meningitis shows a higher incidence in children than adults, but signs may be scarce. Although some pathogenic microorganisms of meningitis from cerebrospinal fluid (CSF) have been reported, the signature of the representative microbiota in CSF and blood samples from patients remains incompletely revealed. To extend the understanding of the microbiome in patients, we recruited 32 children with bacterial meningitis, 30 undiagnosed infectious children, and 10 matched healthy individuals, which was followed by untargeted metagenomic next-generation sequencing (mNGS) and bioinformatic analysis. Our results showed that children with bacterial meningitis exhibited different microbiome signatures in their CSF and blood compared with undiagnosed and healthy children, and patients could be divided into varied subsets according to these signatures, including Escherichia coli, Klebsiella pneumoniae, Thermothelomyces thermophila, Lactobacillus acidophilus, and Staphylococcus haemolyticus. To further explore their potential role in patients’ conditions, we examined their correlation with clinical parameters. Importantly, microbiome signatures with compositional changes were correlated with the C-reactive protein (CRP) level in blood and granulocyte percentage in CSF. Moreover, the blood in subsets of patients with a predominance of Klebsiella pneumoniae could replace CSF as the main specimen for clinical monitoring. IMPORTANCE This study revealed the microbial compositions in children with bacterial meningitis who were treated with antibiotics and made a comprehensive comparison between blood and CSF specimens for the risk and prognosis assessment. We found that microbiome signatures could distinguish patient subsets in the children and were correlated with the CRP level in blood and granulocyte percentage in CSF. The compositional changes in representative microbiota constituents could provide guidance for clinical monitoring and antibiotic intervention.
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spelling pubmed-82692022021-08-02 Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation Liao, Huiping Zhang, Yuchao Guo, Wei Wang, Xi Wang, Hailong Ye, Haocheng Wu, Kai Zhang, Yu-Hang Guo, Lingyun Zhu, Yufei Guo, Yongli Hu, Landian Liu, Gang Kong, Xiangyin mSystems Research Article Bacterial meningitis shows a higher incidence in children than adults, but signs may be scarce. Although some pathogenic microorganisms of meningitis from cerebrospinal fluid (CSF) have been reported, the signature of the representative microbiota in CSF and blood samples from patients remains incompletely revealed. To extend the understanding of the microbiome in patients, we recruited 32 children with bacterial meningitis, 30 undiagnosed infectious children, and 10 matched healthy individuals, which was followed by untargeted metagenomic next-generation sequencing (mNGS) and bioinformatic analysis. Our results showed that children with bacterial meningitis exhibited different microbiome signatures in their CSF and blood compared with undiagnosed and healthy children, and patients could be divided into varied subsets according to these signatures, including Escherichia coli, Klebsiella pneumoniae, Thermothelomyces thermophila, Lactobacillus acidophilus, and Staphylococcus haemolyticus. To further explore their potential role in patients’ conditions, we examined their correlation with clinical parameters. Importantly, microbiome signatures with compositional changes were correlated with the C-reactive protein (CRP) level in blood and granulocyte percentage in CSF. Moreover, the blood in subsets of patients with a predominance of Klebsiella pneumoniae could replace CSF as the main specimen for clinical monitoring. IMPORTANCE This study revealed the microbial compositions in children with bacterial meningitis who were treated with antibiotics and made a comprehensive comparison between blood and CSF specimens for the risk and prognosis assessment. We found that microbiome signatures could distinguish patient subsets in the children and were correlated with the CRP level in blood and granulocyte percentage in CSF. The compositional changes in representative microbiota constituents could provide guidance for clinical monitoring and antibiotic intervention. American Society for Microbiology 2021-06-08 /pmc/articles/PMC8269202/ /pubmed/34100633 http://dx.doi.org/10.1128/mSystems.00049-21 Text en Copyright © 2021 Liao et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Liao, Huiping
Zhang, Yuchao
Guo, Wei
Wang, Xi
Wang, Hailong
Ye, Haocheng
Wu, Kai
Zhang, Yu-Hang
Guo, Lingyun
Zhu, Yufei
Guo, Yongli
Hu, Landian
Liu, Gang
Kong, Xiangyin
Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation
title Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation
title_full Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation
title_fullStr Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation
title_full_unstemmed Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation
title_short Characterization of the Blood and Cerebrospinal Fluid Microbiome in Children with Bacterial Meningitis and Its Potential Correlation with Inflammation
title_sort characterization of the blood and cerebrospinal fluid microbiome in children with bacterial meningitis and its potential correlation with inflammation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269202/
https://www.ncbi.nlm.nih.gov/pubmed/34100633
http://dx.doi.org/10.1128/mSystems.00049-21
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