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The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants
The AP2/EREBP family transcription factors play important roles in a wide range of stress tolerance and hormone signaling. In this study, a heat-inducible rice ERF gene was isolated and functionally characterized. The OsERF115/AP2EREBP110 was categorized to Group-IIIc of the rice AP2/EREBP family an...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269390/ https://www.ncbi.nlm.nih.gov/pubmed/34281241 http://dx.doi.org/10.3390/ijms22137181 |
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author | Park, Seong-Im Kwon, Hyeok Jin Cho, Mi Hyeon Song, Ji Sun Kim, Beom-Gi Baek, JeongHo Kim, Song Lim Ji, HyeonSo Kwon, Taek-Ryoun Kim, Kyung-Hwan Yoon, In Sun |
author_facet | Park, Seong-Im Kwon, Hyeok Jin Cho, Mi Hyeon Song, Ji Sun Kim, Beom-Gi Baek, JeongHo Kim, Song Lim Ji, HyeonSo Kwon, Taek-Ryoun Kim, Kyung-Hwan Yoon, In Sun |
author_sort | Park, Seong-Im |
collection | PubMed |
description | The AP2/EREBP family transcription factors play important roles in a wide range of stress tolerance and hormone signaling. In this study, a heat-inducible rice ERF gene was isolated and functionally characterized. The OsERF115/AP2EREBP110 was categorized to Group-IIIc of the rice AP2/EREBP family and strongly induced by heat and drought treatment. The OsERF115/AP2EREBP110 protein targeted to nuclei and suppressed the ABA-induced transcriptional activation of Rab16A promoter in rice protoplasts. Overexpression of OsERF115/AP2EREBP110 enhanced thermotolerance of seeds and vegetative growth stage plants. The OsERF115/AP2EREBP110 overexpressing (OE) plants exhibited higher proline level and increased expression of a proline biosynthesis P5CS1 gene. Phenotyping of water use dynamics of the individual plant indicates that the OsERF115/AP2EREBP110-OE plant exhibited better water saving traits under heat and drought combined stress. Our combined results suggest the potential use of OsERF115/AP2EREBP110 as a candidate gene for genetic engineering approaches to develop heat and drought stress-tolerant crops. |
format | Online Article Text |
id | pubmed-8269390 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82693902021-07-10 The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants Park, Seong-Im Kwon, Hyeok Jin Cho, Mi Hyeon Song, Ji Sun Kim, Beom-Gi Baek, JeongHo Kim, Song Lim Ji, HyeonSo Kwon, Taek-Ryoun Kim, Kyung-Hwan Yoon, In Sun Int J Mol Sci Article The AP2/EREBP family transcription factors play important roles in a wide range of stress tolerance and hormone signaling. In this study, a heat-inducible rice ERF gene was isolated and functionally characterized. The OsERF115/AP2EREBP110 was categorized to Group-IIIc of the rice AP2/EREBP family and strongly induced by heat and drought treatment. The OsERF115/AP2EREBP110 protein targeted to nuclei and suppressed the ABA-induced transcriptional activation of Rab16A promoter in rice protoplasts. Overexpression of OsERF115/AP2EREBP110 enhanced thermotolerance of seeds and vegetative growth stage plants. The OsERF115/AP2EREBP110 overexpressing (OE) plants exhibited higher proline level and increased expression of a proline biosynthesis P5CS1 gene. Phenotyping of water use dynamics of the individual plant indicates that the OsERF115/AP2EREBP110-OE plant exhibited better water saving traits under heat and drought combined stress. Our combined results suggest the potential use of OsERF115/AP2EREBP110 as a candidate gene for genetic engineering approaches to develop heat and drought stress-tolerant crops. MDPI 2021-07-02 /pmc/articles/PMC8269390/ /pubmed/34281241 http://dx.doi.org/10.3390/ijms22137181 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Park, Seong-Im Kwon, Hyeok Jin Cho, Mi Hyeon Song, Ji Sun Kim, Beom-Gi Baek, JeongHo Kim, Song Lim Ji, HyeonSo Kwon, Taek-Ryoun Kim, Kyung-Hwan Yoon, In Sun The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants |
title | The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants |
title_full | The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants |
title_fullStr | The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants |
title_full_unstemmed | The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants |
title_short | The OsERF115/AP2EREBP110 Transcription Factor Is Involved in the Multiple Stress Tolerance to Heat and Drought in Rice Plants |
title_sort | oserf115/ap2erebp110 transcription factor is involved in the multiple stress tolerance to heat and drought in rice plants |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269390/ https://www.ncbi.nlm.nih.gov/pubmed/34281241 http://dx.doi.org/10.3390/ijms22137181 |
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