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Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing

The textile fragments of the funeral clothes found in the 17th and 18th century crypts were subjected to spectroscopic, spectrometric, and microbial investigation. The next-generation sequencing enabled DNA identification of microorganisms at the genus and in five cases to the species level. The sof...

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Autores principales: Śliwka-Kaszyńska, Magdalena, Ślebioda, Marek, Brillowska-Dąbrowska, Anna, Mroczyńska, Martyna, Karczewski, Jakub, Marzec, Anna, Rybiński, Przemysław, Drążkowska, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269536/
https://www.ncbi.nlm.nih.gov/pubmed/34202830
http://dx.doi.org/10.3390/ma14133535
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author Śliwka-Kaszyńska, Magdalena
Ślebioda, Marek
Brillowska-Dąbrowska, Anna
Mroczyńska, Martyna
Karczewski, Jakub
Marzec, Anna
Rybiński, Przemysław
Drążkowska, Anna
author_facet Śliwka-Kaszyńska, Magdalena
Ślebioda, Marek
Brillowska-Dąbrowska, Anna
Mroczyńska, Martyna
Karczewski, Jakub
Marzec, Anna
Rybiński, Przemysław
Drążkowska, Anna
author_sort Śliwka-Kaszyńska, Magdalena
collection PubMed
description The textile fragments of the funeral clothes found in the 17th and 18th century crypts were subjected to spectroscopic, spectrometric, and microbial investigation. The next-generation sequencing enabled DNA identification of microorganisms at the genus and in five cases to the species level. The soft hydrofluoric acid extraction method was optimized to isolate different classes of dyes from samples that had direct contact with human remains. High-performance liquid chromatography coupled with diode matrix and tandem mass spectrometry detectors with electrospray ionization (HPLC-DAD-ESI-MS/MS) enabled the detection and identification of 34 colourants that are present in historical textiles. Some of them are thus far unknown and uncommon dyes. Indigo, madder, cochineal, turmeric, tannin-producing plant, and young fustic were identified as sources of dyes in textiles. Scanning electron microscopy with energy-dispersive X-ray detector (SEM-EDS) and Fourier transform infrared spectroscopy (FT-IR) were used to identify and characterize fibres and mordants in funeral gowns. Of the 23 textile samples tested, 19 were silk while the remaining four were recognized as wool. The presence of iron, aluminium, sodium, and calcium suggests that they were used as mordants. Traces of copper, silica, and magnesium might originate from the contaminants. The large amount of silver indicated the presence of metal wire in one of the dyed silk textiles. SEM images showed that textile fibres were highly degraded.
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spelling pubmed-82695362021-07-10 Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing Śliwka-Kaszyńska, Magdalena Ślebioda, Marek Brillowska-Dąbrowska, Anna Mroczyńska, Martyna Karczewski, Jakub Marzec, Anna Rybiński, Przemysław Drążkowska, Anna Materials (Basel) Article The textile fragments of the funeral clothes found in the 17th and 18th century crypts were subjected to spectroscopic, spectrometric, and microbial investigation. The next-generation sequencing enabled DNA identification of microorganisms at the genus and in five cases to the species level. The soft hydrofluoric acid extraction method was optimized to isolate different classes of dyes from samples that had direct contact with human remains. High-performance liquid chromatography coupled with diode matrix and tandem mass spectrometry detectors with electrospray ionization (HPLC-DAD-ESI-MS/MS) enabled the detection and identification of 34 colourants that are present in historical textiles. Some of them are thus far unknown and uncommon dyes. Indigo, madder, cochineal, turmeric, tannin-producing plant, and young fustic were identified as sources of dyes in textiles. Scanning electron microscopy with energy-dispersive X-ray detector (SEM-EDS) and Fourier transform infrared spectroscopy (FT-IR) were used to identify and characterize fibres and mordants in funeral gowns. Of the 23 textile samples tested, 19 were silk while the remaining four were recognized as wool. The presence of iron, aluminium, sodium, and calcium suggests that they were used as mordants. Traces of copper, silica, and magnesium might originate from the contaminants. The large amount of silver indicated the presence of metal wire in one of the dyed silk textiles. SEM images showed that textile fibres were highly degraded. MDPI 2021-06-24 /pmc/articles/PMC8269536/ /pubmed/34202830 http://dx.doi.org/10.3390/ma14133535 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Śliwka-Kaszyńska, Magdalena
Ślebioda, Marek
Brillowska-Dąbrowska, Anna
Mroczyńska, Martyna
Karczewski, Jakub
Marzec, Anna
Rybiński, Przemysław
Drążkowska, Anna
Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing
title Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing
title_full Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing
title_fullStr Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing
title_full_unstemmed Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing
title_short Multi-Technique Investigation of Grave Robes from 17th and 18th Century Crypts Using Combined Spectroscopic, Spectrometric Techniques, and New-Generation Sequencing
title_sort multi-technique investigation of grave robes from 17th and 18th century crypts using combined spectroscopic, spectrometric techniques, and new-generation sequencing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269536/
https://www.ncbi.nlm.nih.gov/pubmed/34202830
http://dx.doi.org/10.3390/ma14133535
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