Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk

In this study, the whey protein of fresh mare's milk was used as raw material. The antioxidant peptide liquid XMNDT was extracted from fresh mare's milk solution and purified. The peptide had a molecular weight of 1594.89 kDa and was mainly composed of VAPFPQPVVPYPQR. Antioxidant peptide X...

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Autores principales: Waili, Yizaitiguli, Gahafu, Yiming, Aobulitalifu, Alimijiang, Chang, Zhanying, Xie, Xiangyun, Kawuli, Gulibahaer
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2021
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269580/
https://www.ncbi.nlm.nih.gov/pubmed/34262755
http://dx.doi.org/10.1002/fsn3.2292
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author Waili, Yizaitiguli
Gahafu, Yiming
Aobulitalifu, Alimijiang
Chang, Zhanying
Xie, Xiangyun
Kawuli, Gulibahaer
author_facet Waili, Yizaitiguli
Gahafu, Yiming
Aobulitalifu, Alimijiang
Chang, Zhanying
Xie, Xiangyun
Kawuli, Gulibahaer
author_sort Waili, Yizaitiguli
collection PubMed
description In this study, the whey protein of fresh mare's milk was used as raw material. The antioxidant peptide liquid XMNDT was extracted from fresh mare's milk solution and purified. The peptide had a molecular weight of 1594.89 kDa and was mainly composed of VAPFPQPVVPYPQR. Antioxidant peptide XMNDT could inhibit the proliferation of A549 lung cancer cells in the G1 phase, accelerate cell apoptosis, increase the activity of SOD and the amount of GSH, and reduce the secretion of MDA. It also exhibited certain antioxidant capacity and free radical scavenging. These data can provide a basis for research on new antioxidant properties by reducing the inflammation caused by aging.
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spelling pubmed-82695802021-07-13 Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk Waili, Yizaitiguli Gahafu, Yiming Aobulitalifu, Alimijiang Chang, Zhanying Xie, Xiangyun Kawuli, Gulibahaer Food Sci Nutr Method In this study, the whey protein of fresh mare's milk was used as raw material. The antioxidant peptide liquid XMNDT was extracted from fresh mare's milk solution and purified. The peptide had a molecular weight of 1594.89 kDa and was mainly composed of VAPFPQPVVPYPQR. Antioxidant peptide XMNDT could inhibit the proliferation of A549 lung cancer cells in the G1 phase, accelerate cell apoptosis, increase the activity of SOD and the amount of GSH, and reduce the secretion of MDA. It also exhibited certain antioxidant capacity and free radical scavenging. These data can provide a basis for research on new antioxidant properties by reducing the inflammation caused by aging. John Wiley and Sons Inc. 2021-05-11 /pmc/articles/PMC8269580/ /pubmed/34262755 http://dx.doi.org/10.1002/fsn3.2292 Text en © 2021 The Authors. Food Science & Nutrition published by Wiley Periodicals LLC https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Waili, Yizaitiguli
Gahafu, Yiming
Aobulitalifu, Alimijiang
Chang, Zhanying
Xie, Xiangyun
Kawuli, Gulibahaer
Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk
title Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk
title_full Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk
title_fullStr Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk
title_full_unstemmed Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk
title_short Isolation, purification, and characterization of antioxidant peptides from fresh mare's milk
title_sort isolation, purification, and characterization of antioxidant peptides from fresh mare's milk
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269580/
https://www.ncbi.nlm.nih.gov/pubmed/34262755
http://dx.doi.org/10.1002/fsn3.2292
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