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Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration
The current study presents the effect of naked Fe(3)O(4)@Carbon nanoparticles obtained by the combustion method on primary human gingival fibroblasts (HGFs) and primary gingival keratinocytes (PGKs)—relevant cell lines of buccal oral mucosa. In this regard, the objectives of this study were as follo...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269588/ https://www.ncbi.nlm.nih.gov/pubmed/34202095 http://dx.doi.org/10.3390/ma14133556 |
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author | Pop, Daniel Buzatu, Roxana Moacă, Elena-Alina Watz, Claudia Geanina Cîntă Pînzaru, Simona Barbu Tudoran, Lucian Nekvapil, Fran Avram, Ștefana Dehelean, Cristina Adriana Crețu, Marius Octavian Nicolov, Mirela Szuhanek, Camelia Jivănescu, Anca |
author_facet | Pop, Daniel Buzatu, Roxana Moacă, Elena-Alina Watz, Claudia Geanina Cîntă Pînzaru, Simona Barbu Tudoran, Lucian Nekvapil, Fran Avram, Ștefana Dehelean, Cristina Adriana Crețu, Marius Octavian Nicolov, Mirela Szuhanek, Camelia Jivănescu, Anca |
author_sort | Pop, Daniel |
collection | PubMed |
description | The current study presents the effect of naked Fe(3)O(4)@Carbon nanoparticles obtained by the combustion method on primary human gingival fibroblasts (HGFs) and primary gingival keratinocytes (PGKs)—relevant cell lines of buccal oral mucosa. In this regard, the objectives of this study were as follows: (i) development via combustion method and characterization of nanosized magnetite particles with carbon on their surface, (ii) biocompatibility assessment of the obtained magnetic nanoparticles on HGF and PGK cell lines and (iii) evaluation of possible irritative reaction of Fe(3)O(4)@Carbon nanoparticles on the highly vascularized chorioallantoic membrane of a chick embryo. Physicochemical properties of Fe(3)O(4)@Carbon nanoparticles were characterized in terms of phase composition, chemical structure, and polymorphic and molecular interactions of the chemical bonds within the nanomaterial, magnetic measurements, ultrastructure, morphology, and elemental composition. The X-ray diffraction analysis revealed the formation of magnetite as phase pure without any other secondary phases, and Raman spectroscopy exhibit that the pre-formed magnetic nanoparticles were covered with carbon film, resulting from the synthesis method employed. Scanning electron microscopy shown that nanoparticles obtained were uniformly distributed, with a nearly spherical shape with sizes at the nanometric level; iron, oxygen, and carbon were the only elements detected. While biological screening of Fe(3)O(4)@Carbon nanoparticles revealed no significant cytotoxic potential on the HGF and PGK cell lines, a slight sign of irritation was observed on a limited area on the chorioallantoic membrane of the chick embryo. |
format | Online Article Text |
id | pubmed-8269588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-82695882021-07-10 Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration Pop, Daniel Buzatu, Roxana Moacă, Elena-Alina Watz, Claudia Geanina Cîntă Pînzaru, Simona Barbu Tudoran, Lucian Nekvapil, Fran Avram, Ștefana Dehelean, Cristina Adriana Crețu, Marius Octavian Nicolov, Mirela Szuhanek, Camelia Jivănescu, Anca Materials (Basel) Article The current study presents the effect of naked Fe(3)O(4)@Carbon nanoparticles obtained by the combustion method on primary human gingival fibroblasts (HGFs) and primary gingival keratinocytes (PGKs)—relevant cell lines of buccal oral mucosa. In this regard, the objectives of this study were as follows: (i) development via combustion method and characterization of nanosized magnetite particles with carbon on their surface, (ii) biocompatibility assessment of the obtained magnetic nanoparticles on HGF and PGK cell lines and (iii) evaluation of possible irritative reaction of Fe(3)O(4)@Carbon nanoparticles on the highly vascularized chorioallantoic membrane of a chick embryo. Physicochemical properties of Fe(3)O(4)@Carbon nanoparticles were characterized in terms of phase composition, chemical structure, and polymorphic and molecular interactions of the chemical bonds within the nanomaterial, magnetic measurements, ultrastructure, morphology, and elemental composition. The X-ray diffraction analysis revealed the formation of magnetite as phase pure without any other secondary phases, and Raman spectroscopy exhibit that the pre-formed magnetic nanoparticles were covered with carbon film, resulting from the synthesis method employed. Scanning electron microscopy shown that nanoparticles obtained were uniformly distributed, with a nearly spherical shape with sizes at the nanometric level; iron, oxygen, and carbon were the only elements detected. While biological screening of Fe(3)O(4)@Carbon nanoparticles revealed no significant cytotoxic potential on the HGF and PGK cell lines, a slight sign of irritation was observed on a limited area on the chorioallantoic membrane of the chick embryo. MDPI 2021-06-25 /pmc/articles/PMC8269588/ /pubmed/34202095 http://dx.doi.org/10.3390/ma14133556 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pop, Daniel Buzatu, Roxana Moacă, Elena-Alina Watz, Claudia Geanina Cîntă Pînzaru, Simona Barbu Tudoran, Lucian Nekvapil, Fran Avram, Ștefana Dehelean, Cristina Adriana Crețu, Marius Octavian Nicolov, Mirela Szuhanek, Camelia Jivănescu, Anca Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration |
title | Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration |
title_full | Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration |
title_fullStr | Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration |
title_full_unstemmed | Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration |
title_short | Development and Characterization of Fe(3)O(4)@Carbon Nanoparticles and Their Biological Screening Related to Oral Administration |
title_sort | development and characterization of fe(3)o(4)@carbon nanoparticles and their biological screening related to oral administration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8269588/ https://www.ncbi.nlm.nih.gov/pubmed/34202095 http://dx.doi.org/10.3390/ma14133556 |
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