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Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae

Ayapana triplinervis is a plant species used in traditional medicine and in mystical-religious rituals by traditional communities in the Amazon. The aim of this study are to develop a nano-emulsion containing essential oil from A. triplinervis morphotypes, to evaluate larvicidal activity against Aed...

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Autores principales: Lobato Rodrigues, Alex Bruno, Martins, Rosany Lopes, Rabelo, Érica de Menezes, Tomazi, Rosana, Santos, Lizandra Lima, Brandão, Lethícia Barreto, Faustino, Cleidjane Gomes, Ferreira Farias, Ana Luzia, dos Santos, Cleydson Breno Rodrigues, de Castro Cantuária, Patrick, Galardo, Allan Kardec Ribeiro, de Almeida, Sheylla Susan Moreira da Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270136/
https://www.ncbi.nlm.nih.gov/pubmed/34242328
http://dx.doi.org/10.1371/journal.pone.0254225
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author Lobato Rodrigues, Alex Bruno
Martins, Rosany Lopes
Rabelo, Érica de Menezes
Tomazi, Rosana
Santos, Lizandra Lima
Brandão, Lethícia Barreto
Faustino, Cleidjane Gomes
Ferreira Farias, Ana Luzia
dos Santos, Cleydson Breno Rodrigues
de Castro Cantuária, Patrick
Galardo, Allan Kardec Ribeiro
de Almeida, Sheylla Susan Moreira da Silva
author_facet Lobato Rodrigues, Alex Bruno
Martins, Rosany Lopes
Rabelo, Érica de Menezes
Tomazi, Rosana
Santos, Lizandra Lima
Brandão, Lethícia Barreto
Faustino, Cleidjane Gomes
Ferreira Farias, Ana Luzia
dos Santos, Cleydson Breno Rodrigues
de Castro Cantuária, Patrick
Galardo, Allan Kardec Ribeiro
de Almeida, Sheylla Susan Moreira da Silva
author_sort Lobato Rodrigues, Alex Bruno
collection PubMed
description Ayapana triplinervis is a plant species used in traditional medicine and in mystical-religious rituals by traditional communities in the Amazon. The aim of this study are to develop a nano-emulsion containing essential oil from A. triplinervis morphotypes, to evaluate larvicidal activity against Aedes aegypti and acute oral toxicity in Swiss albino mice (Mus musculus). The essential oils were extracted by steam dragging, identified by gas chromatography coupled to mass spectrometry, and nano-emulsions were prepared using the low energy method. Phytochemical analyses indicated the major compounds, expressed as area percentage, β-Caryophyllene (45.93%) and Thymohydroquinone Dimethyl Ether (32.93%) in morphotype A; and Thymohydroquinone Dimethyl Ether (84.53%) was found in morphotype B. Morphotype A essential oil nano-emulsion showed a particle size of 101.400 ± 0.971 nm (polydispersity index = 0.124 ± 0.009 and zeta potential = -19.300 ± 0.787 mV). Morphotype B essential oil nano-emulsion had a particle size of 104.567 ± 0.416 nm (polydispersity index = 0.168 ± 0.016 and zeta potential = -27.700 ± 1.307 mV). Histomorphological analyses showed the presence of inflammatory cells in the liver of animals treated with morphotype A essential oil nano-emulsion (MAEON) and morphotype B essential oil nano-emulsion (MBEON). Congestion and the presence of transudate with leukocyte infiltration in the lung of animals treated with MAEON were observed. The nano-emulsions containing essential oils of A. triplinervis morphotypes showed an effective nanobiotechnological product in the chemical control of A. aegypti larvae with minimal toxicological action for non-target mammals.
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spelling pubmed-82701362021-07-21 Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae Lobato Rodrigues, Alex Bruno Martins, Rosany Lopes Rabelo, Érica de Menezes Tomazi, Rosana Santos, Lizandra Lima Brandão, Lethícia Barreto Faustino, Cleidjane Gomes Ferreira Farias, Ana Luzia dos Santos, Cleydson Breno Rodrigues de Castro Cantuária, Patrick Galardo, Allan Kardec Ribeiro de Almeida, Sheylla Susan Moreira da Silva PLoS One Research Article Ayapana triplinervis is a plant species used in traditional medicine and in mystical-religious rituals by traditional communities in the Amazon. The aim of this study are to develop a nano-emulsion containing essential oil from A. triplinervis morphotypes, to evaluate larvicidal activity against Aedes aegypti and acute oral toxicity in Swiss albino mice (Mus musculus). The essential oils were extracted by steam dragging, identified by gas chromatography coupled to mass spectrometry, and nano-emulsions were prepared using the low energy method. Phytochemical analyses indicated the major compounds, expressed as area percentage, β-Caryophyllene (45.93%) and Thymohydroquinone Dimethyl Ether (32.93%) in morphotype A; and Thymohydroquinone Dimethyl Ether (84.53%) was found in morphotype B. Morphotype A essential oil nano-emulsion showed a particle size of 101.400 ± 0.971 nm (polydispersity index = 0.124 ± 0.009 and zeta potential = -19.300 ± 0.787 mV). Morphotype B essential oil nano-emulsion had a particle size of 104.567 ± 0.416 nm (polydispersity index = 0.168 ± 0.016 and zeta potential = -27.700 ± 1.307 mV). Histomorphological analyses showed the presence of inflammatory cells in the liver of animals treated with morphotype A essential oil nano-emulsion (MAEON) and morphotype B essential oil nano-emulsion (MBEON). Congestion and the presence of transudate with leukocyte infiltration in the lung of animals treated with MAEON were observed. The nano-emulsions containing essential oils of A. triplinervis morphotypes showed an effective nanobiotechnological product in the chemical control of A. aegypti larvae with minimal toxicological action for non-target mammals. Public Library of Science 2021-07-09 /pmc/articles/PMC8270136/ /pubmed/34242328 http://dx.doi.org/10.1371/journal.pone.0254225 Text en © 2021 Lobato Rodrigues et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Lobato Rodrigues, Alex Bruno
Martins, Rosany Lopes
Rabelo, Érica de Menezes
Tomazi, Rosana
Santos, Lizandra Lima
Brandão, Lethícia Barreto
Faustino, Cleidjane Gomes
Ferreira Farias, Ana Luzia
dos Santos, Cleydson Breno Rodrigues
de Castro Cantuária, Patrick
Galardo, Allan Kardec Ribeiro
de Almeida, Sheylla Susan Moreira da Silva
Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae
title Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae
title_full Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae
title_fullStr Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae
title_full_unstemmed Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae
title_short Development of nano-emulsions based on Ayapana triplinervis essential oil for the control of Aedes aegypti larvae
title_sort development of nano-emulsions based on ayapana triplinervis essential oil for the control of aedes aegypti larvae
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270136/
https://www.ncbi.nlm.nih.gov/pubmed/34242328
http://dx.doi.org/10.1371/journal.pone.0254225
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