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QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population

Maize is a cold sensitive crop that exhibits severe retardation of growth and development when exposed to cold spells during and right after germination, including the slowdown in development of new leaves and in formation of the photosynthetic apparatus. Improving cold tolerance in maize would allo...

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Autores principales: Goering, Raeann, Larsen, Siri, Tan, Jia, Whelan, James, Makarevitch, Irina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270210/
https://www.ncbi.nlm.nih.gov/pubmed/34242344
http://dx.doi.org/10.1371/journal.pone.0254437
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author Goering, Raeann
Larsen, Siri
Tan, Jia
Whelan, James
Makarevitch, Irina
author_facet Goering, Raeann
Larsen, Siri
Tan, Jia
Whelan, James
Makarevitch, Irina
author_sort Goering, Raeann
collection PubMed
description Maize is a cold sensitive crop that exhibits severe retardation of growth and development when exposed to cold spells during and right after germination, including the slowdown in development of new leaves and in formation of the photosynthetic apparatus. Improving cold tolerance in maize would allow early sowing to improve crop yield by prolonging a growing season and by decreasing the negative effects of summer drought, diseases, and pests. Two maize inbreds widely incorporated into American maize germplasm, B73 and Mo17, exhibit different levels of tolerance to low temperature exposure at seedling stage. In addition, thirty seven diverse inbred maize lines showed large variation for seedling response to low temperature exposure with lines with extremely low tolerance to seedling exposure to low temperatures falling into stiff stalk, non-stiff stalk, and tropical clades. We employed the maize intermated B73×Mo17 (IBM) recombinant inbred line population (IBM Syn4 RIL) to investigate the genetic architecture of cold stress tolerance at a young seedling stage and to identify quantitative trait loci (QTLs) controlling this variation. A panel of 97 recombinant inbred lines of IBM Syn4 were used to measure, and score based on several traits related to chlorophyll concentration, leaf color, and tissue damage. Our analysis resulted in detection of two QTLs with high additive impact, one on chromosome 1 (bin 1.02) and second on chromosome 5 (bin 5.05). Further investigation of the QTL regions using gene expression data provided a list of the candidate genes likely contributing to the variation in cold stress response. Among the genes located within QTL regions identified in this study and differentially expressed in response to low temperature exposure are the genes with putative functions related to auxin and gibberellin response, as well as general abiotic stress response, and genes coding for proteins with broad regulatory functions.
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spelling pubmed-82702102021-07-21 QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population Goering, Raeann Larsen, Siri Tan, Jia Whelan, James Makarevitch, Irina PLoS One Research Article Maize is a cold sensitive crop that exhibits severe retardation of growth and development when exposed to cold spells during and right after germination, including the slowdown in development of new leaves and in formation of the photosynthetic apparatus. Improving cold tolerance in maize would allow early sowing to improve crop yield by prolonging a growing season and by decreasing the negative effects of summer drought, diseases, and pests. Two maize inbreds widely incorporated into American maize germplasm, B73 and Mo17, exhibit different levels of tolerance to low temperature exposure at seedling stage. In addition, thirty seven diverse inbred maize lines showed large variation for seedling response to low temperature exposure with lines with extremely low tolerance to seedling exposure to low temperatures falling into stiff stalk, non-stiff stalk, and tropical clades. We employed the maize intermated B73×Mo17 (IBM) recombinant inbred line population (IBM Syn4 RIL) to investigate the genetic architecture of cold stress tolerance at a young seedling stage and to identify quantitative trait loci (QTLs) controlling this variation. A panel of 97 recombinant inbred lines of IBM Syn4 were used to measure, and score based on several traits related to chlorophyll concentration, leaf color, and tissue damage. Our analysis resulted in detection of two QTLs with high additive impact, one on chromosome 1 (bin 1.02) and second on chromosome 5 (bin 5.05). Further investigation of the QTL regions using gene expression data provided a list of the candidate genes likely contributing to the variation in cold stress response. Among the genes located within QTL regions identified in this study and differentially expressed in response to low temperature exposure are the genes with putative functions related to auxin and gibberellin response, as well as general abiotic stress response, and genes coding for proteins with broad regulatory functions. Public Library of Science 2021-07-09 /pmc/articles/PMC8270210/ /pubmed/34242344 http://dx.doi.org/10.1371/journal.pone.0254437 Text en © 2021 Goering et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Goering, Raeann
Larsen, Siri
Tan, Jia
Whelan, James
Makarevitch, Irina
QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population
title QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population
title_full QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population
title_fullStr QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population
title_full_unstemmed QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population
title_short QTL mapping of seedling tolerance to exposure to low temperature in the maize IBM RIL population
title_sort qtl mapping of seedling tolerance to exposure to low temperature in the maize ibm ril population
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270210/
https://www.ncbi.nlm.nih.gov/pubmed/34242344
http://dx.doi.org/10.1371/journal.pone.0254437
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