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Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases

BACKGROUND: Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature revi...

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Autores principales: Chan, YuYen, Fornace, Kimberly, Wu, Lindsey, Arnold, Benjamin F., Priest, Jeffrey W., Martin, Diana L., Chang, Michelle A., Cook, Jackie, Stresman, Gillian, Drakeley, Chris
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270565/
https://www.ncbi.nlm.nih.gov/pubmed/34181665
http://dx.doi.org/10.1371/journal.pntd.0009457
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author Chan, YuYen
Fornace, Kimberly
Wu, Lindsey
Arnold, Benjamin F.
Priest, Jeffrey W.
Martin, Diana L.
Chang, Michelle A.
Cook, Jackie
Stresman, Gillian
Drakeley, Chris
author_facet Chan, YuYen
Fornace, Kimberly
Wu, Lindsey
Arnold, Benjamin F.
Priest, Jeffrey W.
Martin, Diana L.
Chang, Michelle A.
Cook, Jackie
Stresman, Gillian
Drakeley, Chris
author_sort Chan, YuYen
collection PubMed
description BACKGROUND: Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature review was conducted to describe the methods used to determine cut-off values for data generated by multiplex bead assays. METHODOLOGY/PRINCIPAL FINDINGS: A search was conducted in PubMed that included articles published from January 2010 to January 2020, and 308 relevant articles were identified that included the terms “serology”, “cut-offs”, and “multiplex bead assays”. After application of exclusion of articles not relevant to neglected tropical diseases (NTD), vaccine preventable diseases (VPD), or malaria, 55 articles were examined based on their relevance to NTD or VPD. The most frequently applied approaches to determine seropositivity included the use of presumed unexposed populations, mixture models, receiver operating curves (ROC), and international standards. Other methods included the use of quantiles, pre-exposed endemic cohorts, and visual inflection points. CONCLUSIONS/SIGNIFICANCE: For disease control programmes, seropositivity is a practical and easily interpretable health metric but determining appropriate cut-offs for positivity can be challenging. Considerations for optimal cut-off approaches should include factors such as methods recommended by previous research, transmission dynamics, and the immunological backgrounds of the population. In the absence of international standards for estimating seropositivity in a population, the use of consistent methods that align with individual disease epidemiological data will improve comparability between settings and enable the assessment of changes over time.
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spelling pubmed-82705652021-07-21 Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases Chan, YuYen Fornace, Kimberly Wu, Lindsey Arnold, Benjamin F. Priest, Jeffrey W. Martin, Diana L. Chang, Michelle A. Cook, Jackie Stresman, Gillian Drakeley, Chris PLoS Negl Trop Dis Research Article BACKGROUND: Serological surveys with multiplex bead assays can be used to assess seroprevalence to multiple pathogens simultaneously. However, multiple methods have been used to generate cut-off values for seropositivity and these may lead to inconsistent interpretation of results. A literature review was conducted to describe the methods used to determine cut-off values for data generated by multiplex bead assays. METHODOLOGY/PRINCIPAL FINDINGS: A search was conducted in PubMed that included articles published from January 2010 to January 2020, and 308 relevant articles were identified that included the terms “serology”, “cut-offs”, and “multiplex bead assays”. After application of exclusion of articles not relevant to neglected tropical diseases (NTD), vaccine preventable diseases (VPD), or malaria, 55 articles were examined based on their relevance to NTD or VPD. The most frequently applied approaches to determine seropositivity included the use of presumed unexposed populations, mixture models, receiver operating curves (ROC), and international standards. Other methods included the use of quantiles, pre-exposed endemic cohorts, and visual inflection points. CONCLUSIONS/SIGNIFICANCE: For disease control programmes, seropositivity is a practical and easily interpretable health metric but determining appropriate cut-offs for positivity can be challenging. Considerations for optimal cut-off approaches should include factors such as methods recommended by previous research, transmission dynamics, and the immunological backgrounds of the population. In the absence of international standards for estimating seropositivity in a population, the use of consistent methods that align with individual disease epidemiological data will improve comparability between settings and enable the assessment of changes over time. Public Library of Science 2021-06-28 /pmc/articles/PMC8270565/ /pubmed/34181665 http://dx.doi.org/10.1371/journal.pntd.0009457 Text en https://creativecommons.org/publicdomain/zero/1.0/This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 (https://creativecommons.org/publicdomain/zero/1.0/) public domain dedication.
spellingShingle Research Article
Chan, YuYen
Fornace, Kimberly
Wu, Lindsey
Arnold, Benjamin F.
Priest, Jeffrey W.
Martin, Diana L.
Chang, Michelle A.
Cook, Jackie
Stresman, Gillian
Drakeley, Chris
Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases
title Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases
title_full Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases
title_fullStr Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases
title_full_unstemmed Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases
title_short Determining seropositivity—A review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases
title_sort determining seropositivity—a review of approaches to define population seroprevalence when using multiplex bead assays to assess burden of tropical diseases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270565/
https://www.ncbi.nlm.nih.gov/pubmed/34181665
http://dx.doi.org/10.1371/journal.pntd.0009457
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