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Skin-on-a-chip models: General overview and future perspectives

Over the last few years, several advances have been made toward the development and production of in vitro human skin models for the analysis and testing of cosmetic and pharmaceutical products. However, these skin models are cultured under static conditions that make them unable to accurately repre...

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Autores principales: Risueño, I., Valencia, L., Jorcano, J. L., Velasco, D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270645/
https://www.ncbi.nlm.nih.gov/pubmed/34258497
http://dx.doi.org/10.1063/5.0046376
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author Risueño, I.
Valencia, L.
Jorcano, J. L.
Velasco, D.
author_facet Risueño, I.
Valencia, L.
Jorcano, J. L.
Velasco, D.
author_sort Risueño, I.
collection PubMed
description Over the last few years, several advances have been made toward the development and production of in vitro human skin models for the analysis and testing of cosmetic and pharmaceutical products. However, these skin models are cultured under static conditions that make them unable to accurately represent normal human physiology. Recent interest has focused on the generation of in vitro 3D vascularized skin models with dynamic perfusion and microfluidic devices known as skin-on-a-chip. These platforms have been widely described in the literature as good candidates for tissue modeling, as they enable a more physiological transport of nutrients and permit a high-throughput and less expensive evaluation of drug candidates in terms of toxicity, efficacy, and delivery. In this Perspective, recent advances in these novel platforms for the generation of human skin models under dynamic conditions for in vitro testing are reported. Advances in vascularized human skin equivalents (HSEs), transferred skin-on-a-chip (introduction of a skin biopsy or a HSE in the chip), and in situ skin-on-a-chip (generation of the skin model directly in the chip) are critically reviewed, and currently used methods for the introduction of skin cells in the microfluidic chips are discussed. An outlook on current applications and future directions in this field of research are also presented.
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spelling pubmed-82706452021-07-12 Skin-on-a-chip models: General overview and future perspectives Risueño, I. Valencia, L. Jorcano, J. L. Velasco, D. APL Bioeng Perspectives Over the last few years, several advances have been made toward the development and production of in vitro human skin models for the analysis and testing of cosmetic and pharmaceutical products. However, these skin models are cultured under static conditions that make them unable to accurately represent normal human physiology. Recent interest has focused on the generation of in vitro 3D vascularized skin models with dynamic perfusion and microfluidic devices known as skin-on-a-chip. These platforms have been widely described in the literature as good candidates for tissue modeling, as they enable a more physiological transport of nutrients and permit a high-throughput and less expensive evaluation of drug candidates in terms of toxicity, efficacy, and delivery. In this Perspective, recent advances in these novel platforms for the generation of human skin models under dynamic conditions for in vitro testing are reported. Advances in vascularized human skin equivalents (HSEs), transferred skin-on-a-chip (introduction of a skin biopsy or a HSE in the chip), and in situ skin-on-a-chip (generation of the skin model directly in the chip) are critically reviewed, and currently used methods for the introduction of skin cells in the microfluidic chips are discussed. An outlook on current applications and future directions in this field of research are also presented. AIP Publishing LLC 2021-07-08 /pmc/articles/PMC8270645/ /pubmed/34258497 http://dx.doi.org/10.1063/5.0046376 Text en © 2021 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Perspectives
Risueño, I.
Valencia, L.
Jorcano, J. L.
Velasco, D.
Skin-on-a-chip models: General overview and future perspectives
title Skin-on-a-chip models: General overview and future perspectives
title_full Skin-on-a-chip models: General overview and future perspectives
title_fullStr Skin-on-a-chip models: General overview and future perspectives
title_full_unstemmed Skin-on-a-chip models: General overview and future perspectives
title_short Skin-on-a-chip models: General overview and future perspectives
title_sort skin-on-a-chip models: general overview and future perspectives
topic Perspectives
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270645/
https://www.ncbi.nlm.nih.gov/pubmed/34258497
http://dx.doi.org/10.1063/5.0046376
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