Cargando…

Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells

OBJECTIVE: To investigate the role of PI3K/AKT signaling pathway in nucleus pulposus (NP) cells. METHODS: Nucleus pulposus (NP) cells were isolated from SD rat, and thereafter, passage three (P3) NP cells were divided into the following experimental groups: control, PI3K/AKT agonist IGF-1 (25 ng/ml,...

Descripción completa

Detalles Bibliográficos
Autores principales: Xiao, Quan, Teng, Yun, Xu, Changming, Pan, Wei, Yang, Hanshi, Zhao, Jiali, Zhou, Quan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270693/
https://www.ncbi.nlm.nih.gov/pubmed/34307680
http://dx.doi.org/10.1155/2021/9941253
_version_ 1783720846523629568
author Xiao, Quan
Teng, Yun
Xu, Changming
Pan, Wei
Yang, Hanshi
Zhao, Jiali
Zhou, Quan
author_facet Xiao, Quan
Teng, Yun
Xu, Changming
Pan, Wei
Yang, Hanshi
Zhao, Jiali
Zhou, Quan
author_sort Xiao, Quan
collection PubMed
description OBJECTIVE: To investigate the role of PI3K/AKT signaling pathway in nucleus pulposus (NP) cells. METHODS: Nucleus pulposus (NP) cells were isolated from SD rat, and thereafter, passage three (P3) NP cells were divided into the following experimental groups: control, PI3K/AKT agonist IGF-1 (25 ng/ml, 50 ng/ml, and 100 ng/ml), and PI3K/AKT inhibitor LY294002 (5 μM, 10 μM, and 20 μM). Flow cytometry and BrdU cell proliferation assays were performed to assess apoptosis and the proliferation rate of NP cells. Western blot analysis was performed to examine the protein expression level of Col II, Col X, Aggrecan, and MMP13. RESULTS: PI3K/AKT inhibitor LY294002 increased the rate of apoptosis in NP cells when compared to the control and decreased the proliferation rate when compared to control. Moreover, LY294002 decreased the protein expression level of Col-II and Aggrecan in NP cells. At the same time, LY294002 increased the protein expression level of MMP13 and Col-X in NP cells. Through activating PI3K/AKT, IGF-1 increased the proliferation rate when compared to control and decreased the rate of apoptosis when compared to control. Additionally, IGF-1 decreased the protein expression level of MMP13 and Col-X and increased Col-II and Aggrecan in NP cells. CONCLUSION: The inhibition of PI3K/AKT signaling pathway accelerated the apoptosis of NP cells and facilitated the extracellular matrix degradation. However, the activation of PI3K/AKT pathway partly prevented the NP cell from apoptosis and promoted their proliferation. Meanwhile, its activation also delayed the loss of extracellular matrix.
format Online
Article
Text
id pubmed-8270693
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Hindawi
record_format MEDLINE/PubMed
spelling pubmed-82706932021-07-22 Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells Xiao, Quan Teng, Yun Xu, Changming Pan, Wei Yang, Hanshi Zhao, Jiali Zhou, Quan Biomed Res Int Research Article OBJECTIVE: To investigate the role of PI3K/AKT signaling pathway in nucleus pulposus (NP) cells. METHODS: Nucleus pulposus (NP) cells were isolated from SD rat, and thereafter, passage three (P3) NP cells were divided into the following experimental groups: control, PI3K/AKT agonist IGF-1 (25 ng/ml, 50 ng/ml, and 100 ng/ml), and PI3K/AKT inhibitor LY294002 (5 μM, 10 μM, and 20 μM). Flow cytometry and BrdU cell proliferation assays were performed to assess apoptosis and the proliferation rate of NP cells. Western blot analysis was performed to examine the protein expression level of Col II, Col X, Aggrecan, and MMP13. RESULTS: PI3K/AKT inhibitor LY294002 increased the rate of apoptosis in NP cells when compared to the control and decreased the proliferation rate when compared to control. Moreover, LY294002 decreased the protein expression level of Col-II and Aggrecan in NP cells. At the same time, LY294002 increased the protein expression level of MMP13 and Col-X in NP cells. Through activating PI3K/AKT, IGF-1 increased the proliferation rate when compared to control and decreased the rate of apoptosis when compared to control. Additionally, IGF-1 decreased the protein expression level of MMP13 and Col-X and increased Col-II and Aggrecan in NP cells. CONCLUSION: The inhibition of PI3K/AKT signaling pathway accelerated the apoptosis of NP cells and facilitated the extracellular matrix degradation. However, the activation of PI3K/AKT pathway partly prevented the NP cell from apoptosis and promoted their proliferation. Meanwhile, its activation also delayed the loss of extracellular matrix. Hindawi 2021-07-01 /pmc/articles/PMC8270693/ /pubmed/34307680 http://dx.doi.org/10.1155/2021/9941253 Text en Copyright © 2021 Quan Xiao et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Xiao, Quan
Teng, Yun
Xu, Changming
Pan, Wei
Yang, Hanshi
Zhao, Jiali
Zhou, Quan
Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells
title Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells
title_full Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells
title_fullStr Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells
title_full_unstemmed Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells
title_short Role of PI3K/AKT Signaling Pathway in Nucleus Pulposus Cells
title_sort role of pi3k/akt signaling pathway in nucleus pulposus cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270693/
https://www.ncbi.nlm.nih.gov/pubmed/34307680
http://dx.doi.org/10.1155/2021/9941253
work_keys_str_mv AT xiaoquan roleofpi3kaktsignalingpathwayinnucleuspulposuscells
AT tengyun roleofpi3kaktsignalingpathwayinnucleuspulposuscells
AT xuchangming roleofpi3kaktsignalingpathwayinnucleuspulposuscells
AT panwei roleofpi3kaktsignalingpathwayinnucleuspulposuscells
AT yanghanshi roleofpi3kaktsignalingpathwayinnucleuspulposuscells
AT zhaojiali roleofpi3kaktsignalingpathwayinnucleuspulposuscells
AT zhouquan roleofpi3kaktsignalingpathwayinnucleuspulposuscells