Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae

A temperate siphovirus, phiCDKH01, was obtained from a clinical isolate of Clostridioides difficile. The phage genome is a 45,089-bp linear double-stranded DNA molecule with an average G+C content of 28.7%. It shows low similarity to known phage genomes, except for phiCD24-1. Genomic and phylogeneti...

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Autores principales: Hinc, Krzysztof, Kabała, Monika, Iwanicki, Adam, Martirosian, Gajane, Negri, Alessandro, Obuchowski, Michał
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Vienna 2021
Materias:
Annotated Sequence Record
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270841/
https://www.ncbi.nlm.nih.gov/pubmed/34014385
http://dx.doi.org/10.1007/s00705-021-05092-0
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author Hinc, Krzysztof
Kabała, Monika
Iwanicki, Adam
Martirosian, Gajane
Negri, Alessandro
Obuchowski, Michał
author_facet Hinc, Krzysztof
Kabała, Monika
Iwanicki, Adam
Martirosian, Gajane
Negri, Alessandro
Obuchowski, Michał
author_sort Hinc, Krzysztof
collection PubMed
description A temperate siphovirus, phiCDKH01, was obtained from a clinical isolate of Clostridioides difficile. The phage genome is a 45,089-bp linear double-stranded DNA molecule with an average G+C content of 28.7%. It shows low similarity to known phage genomes, except for phiCD24-1. Genomic and phylogenetic analysis revealed that phiCDKH01 is a newly discovered phage. Sixty-six putative ORFs were predicted in the genome, 37 of which code for proteins with predicted functions. The phiCDKH01 prophage was localized in the host genome. The results of this study increase our knowledge about the genetic diversity of tailed phages. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05092-0.
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spelling pubmed-82708412021-07-20 Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae Hinc, Krzysztof Kabała, Monika Iwanicki, Adam Martirosian, Gajane Negri, Alessandro Obuchowski, Michał Arch Virol Annotated Sequence Record A temperate siphovirus, phiCDKH01, was obtained from a clinical isolate of Clostridioides difficile. The phage genome is a 45,089-bp linear double-stranded DNA molecule with an average G+C content of 28.7%. It shows low similarity to known phage genomes, except for phiCD24-1. Genomic and phylogenetic analysis revealed that phiCDKH01 is a newly discovered phage. Sixty-six putative ORFs were predicted in the genome, 37 of which code for proteins with predicted functions. The phiCDKH01 prophage was localized in the host genome. The results of this study increase our knowledge about the genetic diversity of tailed phages. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00705-021-05092-0. Springer Vienna 2021-05-20 2021 /pmc/articles/PMC8270841/ /pubmed/34014385 http://dx.doi.org/10.1007/s00705-021-05092-0 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Annotated Sequence Record
Hinc, Krzysztof
Kabała, Monika
Iwanicki, Adam
Martirosian, Gajane
Negri, Alessandro
Obuchowski, Michał
Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae
title Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae
title_full Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae
title_fullStr Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae
title_full_unstemmed Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae
title_short Complete genome sequence of the newly discovered temperate Clostridioides difficile bacteriophage phiCDKH01 of the family Siphoviridae
title_sort complete genome sequence of the newly discovered temperate clostridioides difficile bacteriophage phicdkh01 of the family siphoviridae
topic Annotated Sequence Record
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270841/
https://www.ncbi.nlm.nih.gov/pubmed/34014385
http://dx.doi.org/10.1007/s00705-021-05092-0
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