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Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae

Olfactory sensing is generally organized into groups of similarly sensing olfactory receptor neurons converging into their corresponding glomerulus, which is thought to behave as a uniform functional unit. It is however unclear to which degree axons within a glomerulus show identical activity, how m...

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Autores principales: Topci, Rodi, Alevra, Mihai, Rauf, Erik H. U., de Jong-Bolm, Daniëlle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270923/
https://www.ncbi.nlm.nih.gov/pubmed/34244587
http://dx.doi.org/10.1038/s41598-021-93677-9
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author Topci, Rodi
Alevra, Mihai
Rauf, Erik H. U.
de Jong-Bolm, Daniëlle
author_facet Topci, Rodi
Alevra, Mihai
Rauf, Erik H. U.
de Jong-Bolm, Daniëlle
author_sort Topci, Rodi
collection PubMed
description Olfactory sensing is generally organized into groups of similarly sensing olfactory receptor neurons converging into their corresponding glomerulus, which is thought to behave as a uniform functional unit. It is however unclear to which degree axons within a glomerulus show identical activity, how many converge into a glomerulus, and to answer these questions, how it is possible to visually separate them in live imaging. Here we investigate activity of olfactory receptor neurons and their axon terminals throughout olfactory glomeruli using electrophysiological recordings and rapid 4D calcium imaging. While single olfactory receptor neurons responsive to the same odor stimulus show a diversity of responses in terms of sensitivity and spontaneous firing rate on the level of the somata, their pre-synaptic calcium activity in the glomerulus is homogeneous. In addition, we could not observe the correlated spontaneous calcium activity that is found on the post-synaptic side throughout mitral cell dendrites and has been used in activity correlation imaging. However, it is possible to induce spatio-temporal presynaptic response inhomogeneities by applying trains of olfactory stimuli with varying amino acid concentrations. Automated region-of-interest detection and correlation analysis then visually distinguishes at least two axon subgroups per glomerulus that differ in odor sensitivity.
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spelling pubmed-82709232021-07-12 Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae Topci, Rodi Alevra, Mihai Rauf, Erik H. U. de Jong-Bolm, Daniëlle Sci Rep Article Olfactory sensing is generally organized into groups of similarly sensing olfactory receptor neurons converging into their corresponding glomerulus, which is thought to behave as a uniform functional unit. It is however unclear to which degree axons within a glomerulus show identical activity, how many converge into a glomerulus, and to answer these questions, how it is possible to visually separate them in live imaging. Here we investigate activity of olfactory receptor neurons and their axon terminals throughout olfactory glomeruli using electrophysiological recordings and rapid 4D calcium imaging. While single olfactory receptor neurons responsive to the same odor stimulus show a diversity of responses in terms of sensitivity and spontaneous firing rate on the level of the somata, their pre-synaptic calcium activity in the glomerulus is homogeneous. In addition, we could not observe the correlated spontaneous calcium activity that is found on the post-synaptic side throughout mitral cell dendrites and has been used in activity correlation imaging. However, it is possible to induce spatio-temporal presynaptic response inhomogeneities by applying trains of olfactory stimuli with varying amino acid concentrations. Automated region-of-interest detection and correlation analysis then visually distinguishes at least two axon subgroups per glomerulus that differ in odor sensitivity. Nature Publishing Group UK 2021-07-09 /pmc/articles/PMC8270923/ /pubmed/34244587 http://dx.doi.org/10.1038/s41598-021-93677-9 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Topci, Rodi
Alevra, Mihai
Rauf, Erik H. U.
de Jong-Bolm, Daniëlle
Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_full Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_fullStr Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_full_unstemmed Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_short Resolving different presynaptic activity patterns within single olfactory glomeruli of Xenopus laevis larvae
title_sort resolving different presynaptic activity patterns within single olfactory glomeruli of xenopus laevis larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8270923/
https://www.ncbi.nlm.nih.gov/pubmed/34244587
http://dx.doi.org/10.1038/s41598-021-93677-9
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