Cargando…

Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone

Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various c...

Descripción completa

Detalles Bibliográficos
Autores principales: Zaid, Ahmed S. Abu, Aleissawy, Ahmed E., Yahia, Ibrahim S., Yassien, Mahmoud A., Hassouna, Nadia A., Aboshanab, Khaled M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271628/
https://www.ncbi.nlm.nih.gov/pubmed/34209170
http://dx.doi.org/10.3390/molecules26134009
_version_ 1783721043714637824
author Zaid, Ahmed S. Abu
Aleissawy, Ahmed E.
Yahia, Ibrahim S.
Yassien, Mahmoud A.
Hassouna, Nadia A.
Aboshanab, Khaled M.
author_facet Zaid, Ahmed S. Abu
Aleissawy, Ahmed E.
Yahia, Ibrahim S.
Yassien, Mahmoud A.
Hassouna, Nadia A.
Aboshanab, Khaled M.
author_sort Zaid, Ahmed S. Abu
collection PubMed
description Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various cancer cell lines. Methods: Production of cytotoxic metabolites from S. griseus isolate KJ623766 was carried out in a 14L laboratory fermenter under specified optimum conditions. Using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined. Bioassay-guided fractionation of the ethyl acetate extract using different chromatographic techniques was used for cytotoxic metabolite purification. Chemical structures of the purified metabolites were identified using mass, 1D, and 2D NMR spectroscopic analysis. Results: Bioassay-guided fractionation of the ethyl acetate extract led to the purification of two cytotoxic metabolites, R1 and R2, of reproducible amounts of 5 and 1.5 mg/L, respectively. The structures of R1 and R2 metabolites were identified as β- and γ-rhodomycinone with CD(50) of 6.3, 9.45, 64.8 and 9.11, 9.35, 67.3 µg/mL against Caco2, Hela and Vero cell lines, respectively. Values were comparable to those of the positive control doxorubicin. Conclusions: This is the first report about the production of β- and γ-rhodomycinone, two important scaffolds for synthesis of anticancer drugs, from S. griseus.
format Online
Article
Text
id pubmed-8271628
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-82716282021-07-11 Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone Zaid, Ahmed S. Abu Aleissawy, Ahmed E. Yahia, Ibrahim S. Yassien, Mahmoud A. Hassouna, Nadia A. Aboshanab, Khaled M. Molecules Article Background: This study aimed to produce, purify, structurally elucidate, and explore the biological activities of metabolites produced by Streptomyces (S.) griseus isolate KJ623766, a recovered soil bacterium previously screened in our lab that showed promising cytotoxic activities against various cancer cell lines. Methods: Production of cytotoxic metabolites from S. griseus isolate KJ623766 was carried out in a 14L laboratory fermenter under specified optimum conditions. Using a 3-(4,5-dimethylthazol-2-yl)-2,5-diphenyl tetrazolium-bromide assay, the cytotoxic activity of the ethyl acetate extract against Caco2 and Hela cancer cell lines was determined. Bioassay-guided fractionation of the ethyl acetate extract using different chromatographic techniques was used for cytotoxic metabolite purification. Chemical structures of the purified metabolites were identified using mass, 1D, and 2D NMR spectroscopic analysis. Results: Bioassay-guided fractionation of the ethyl acetate extract led to the purification of two cytotoxic metabolites, R1 and R2, of reproducible amounts of 5 and 1.5 mg/L, respectively. The structures of R1 and R2 metabolites were identified as β- and γ-rhodomycinone with CD(50) of 6.3, 9.45, 64.8 and 9.11, 9.35, 67.3 µg/mL against Caco2, Hela and Vero cell lines, respectively. Values were comparable to those of the positive control doxorubicin. Conclusions: This is the first report about the production of β- and γ-rhodomycinone, two important scaffolds for synthesis of anticancer drugs, from S. griseus. MDPI 2021-06-30 /pmc/articles/PMC8271628/ /pubmed/34209170 http://dx.doi.org/10.3390/molecules26134009 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zaid, Ahmed S. Abu
Aleissawy, Ahmed E.
Yahia, Ibrahim S.
Yassien, Mahmoud A.
Hassouna, Nadia A.
Aboshanab, Khaled M.
Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone
title Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone
title_full Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone
title_fullStr Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone
title_full_unstemmed Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone
title_short Streptomyces griseus KJ623766: A Natural Producer of Two Anthracycline Cytotoxic Metabolites β- and γ-Rhodomycinone
title_sort streptomyces griseus kj623766: a natural producer of two anthracycline cytotoxic metabolites β- and γ-rhodomycinone
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271628/
https://www.ncbi.nlm.nih.gov/pubmed/34209170
http://dx.doi.org/10.3390/molecules26134009
work_keys_str_mv AT zaidahmedsabu streptomycesgriseuskj623766anaturalproduceroftwoanthracyclinecytotoxicmetabolitesbandgrhodomycinone
AT aleissawyahmede streptomycesgriseuskj623766anaturalproduceroftwoanthracyclinecytotoxicmetabolitesbandgrhodomycinone
AT yahiaibrahims streptomycesgriseuskj623766anaturalproduceroftwoanthracyclinecytotoxicmetabolitesbandgrhodomycinone
AT yassienmahmouda streptomycesgriseuskj623766anaturalproduceroftwoanthracyclinecytotoxicmetabolitesbandgrhodomycinone
AT hassounanadiaa streptomycesgriseuskj623766anaturalproduceroftwoanthracyclinecytotoxicmetabolitesbandgrhodomycinone
AT aboshanabkhaledm streptomycesgriseuskj623766anaturalproduceroftwoanthracyclinecytotoxicmetabolitesbandgrhodomycinone