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Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors

Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the r...

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Autores principales: Mrozek, Piotr, Gorodkiewicz, Ewa, Falkowski, Paweł, Hościło, Bogusław
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271734/
https://www.ncbi.nlm.nih.gov/pubmed/34202104
http://dx.doi.org/10.3390/s21134348
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author Mrozek, Piotr
Gorodkiewicz, Ewa
Falkowski, Paweł
Hościło, Bogusław
author_facet Mrozek, Piotr
Gorodkiewicz, Ewa
Falkowski, Paweł
Hościło, Bogusław
author_sort Mrozek, Piotr
collection PubMed
description Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the results of experimental reflectance measurement of real-life systems, were used for the analysis of these sensors. Mutual relationships were determined between ∂Y/∂n components of sensitivity of the Y signal in the SPR measurement to change the refractive index n of the near-surface sensing layer and ∂n/∂c sensitivity of refractive index n to change the analyte’s concentration, c, for both types of sensors. Obtained results were related to experimentally determined calibration curves of both sensors. A characteristic feature arising from the comparison of calibration curves is the similar level of Au and Ag–Au biosensors’ sensitivity in the linear range, where the signal of the AgAu sensor is at a level several times greater. It was shown that the influence of sensing surface morphology on the ∂n/∂c sensitivity component had to be incorporated to explain the features of calibration curves of sensors. The shape of the sensory surface relief was proposed to increase the sensor sensitivity at low analyte concentrations.
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spelling pubmed-82717342021-07-11 Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors Mrozek, Piotr Gorodkiewicz, Ewa Falkowski, Paweł Hościło, Bogusław Sensors (Basel) Article Comparative analysis of the sensitivity of two surface plasmon resonance (SPR) biosensors was conducted on a single-metallic Au sensor and bimetallic Ag–Au sensor, using a cathepsin S sensor as an example. Numerically modeled resonance curves of Au and Ag–Au layers, with parameters verified by the results of experimental reflectance measurement of real-life systems, were used for the analysis of these sensors. Mutual relationships were determined between ∂Y/∂n components of sensitivity of the Y signal in the SPR measurement to change the refractive index n of the near-surface sensing layer and ∂n/∂c sensitivity of refractive index n to change the analyte’s concentration, c, for both types of sensors. Obtained results were related to experimentally determined calibration curves of both sensors. A characteristic feature arising from the comparison of calibration curves is the similar level of Au and Ag–Au biosensors’ sensitivity in the linear range, where the signal of the AgAu sensor is at a level several times greater. It was shown that the influence of sensing surface morphology on the ∂n/∂c sensitivity component had to be incorporated to explain the features of calibration curves of sensors. The shape of the sensory surface relief was proposed to increase the sensor sensitivity at low analyte concentrations. MDPI 2021-06-25 /pmc/articles/PMC8271734/ /pubmed/34202104 http://dx.doi.org/10.3390/s21134348 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mrozek, Piotr
Gorodkiewicz, Ewa
Falkowski, Paweł
Hościło, Bogusław
Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
title Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
title_full Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
title_fullStr Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
title_full_unstemmed Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
title_short Sensitivity Analysis of Single- and Bimetallic Surface Plasmon Resonance Biosensors
title_sort sensitivity analysis of single- and bimetallic surface plasmon resonance biosensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8271734/
https://www.ncbi.nlm.nih.gov/pubmed/34202104
http://dx.doi.org/10.3390/s21134348
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