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Benchmark of 16S rRNA gene amplicon sequencing using Japanese gut microbiome data from the V1–V2 and V3–V4 primer sets

BACKGROUND: 16S rRNA gene amplicon sequencing (16S analysis) is widely used to analyze microbiota with next-generation sequencing technologies. Here, we compared fecal 16S analysis data from 192 Japanese volunteers using the modified V1–V2 (V12) and the standard V3–V4 primer (V34) sets to optimize t...

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Detalles Bibliográficos
Autores principales: Kameoka, Shoichiro, Motooka, Daisuke, Watanabe, Satoshi, Kubo, Ryuichi, Jung, Nicolas, Midorikawa, Yuki, Shinozaki, Natsuko O., Sawai, Yu, Takeda, Aya K., Nakamura, Shota
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272389/
https://www.ncbi.nlm.nih.gov/pubmed/34246242
http://dx.doi.org/10.1186/s12864-021-07746-4