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Benchmark of 16S rRNA gene amplicon sequencing using Japanese gut microbiome data from the V1–V2 and V3–V4 primer sets
BACKGROUND: 16S rRNA gene amplicon sequencing (16S analysis) is widely used to analyze microbiota with next-generation sequencing technologies. Here, we compared fecal 16S analysis data from 192 Japanese volunteers using the modified V1–V2 (V12) and the standard V3–V4 primer (V34) sets to optimize t...
Autores principales: | Kameoka, Shoichiro, Motooka, Daisuke, Watanabe, Satoshi, Kubo, Ryuichi, Jung, Nicolas, Midorikawa, Yuki, Shinozaki, Natsuko O., Sawai, Yu, Takeda, Aya K., Nakamura, Shota |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8272389/ https://www.ncbi.nlm.nih.gov/pubmed/34246242 http://dx.doi.org/10.1186/s12864-021-07746-4 |
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