A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids

Systematic approaches for functionally validating cancer genes are needed since numerous genes mutated in cancer tissues have been identified from cancer genome sequencing. The mouse organoid culture system has been extensively used in the field of cancer research since mouse organoids can faithfull...

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Detalles Bibliográficos
Autor principal: Takeda, Haruna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273277/
https://www.ncbi.nlm.nih.gov/pubmed/34262603
http://dx.doi.org/10.3389/fgene.2021.698771
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author Takeda, Haruna
author_facet Takeda, Haruna
author_sort Takeda, Haruna
collection PubMed
description Systematic approaches for functionally validating cancer genes are needed since numerous genes mutated in cancer tissues have been identified from cancer genome sequencing. The mouse organoid culture system has been extensively used in the field of cancer research since mouse organoids can faithfully recapitulate the physiological behavior of the cells. Taking advantage of this, we recently described a platform for functionally validating colorectal cancer (CRC) driver genes that utilized CRISPR-Cas9 in mouse intestinal tumor organoids. In this review, we will describe how mouse organoids have been applied to CRC research and focus on how CRC genes can be validated using mouse organoids.
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spelling pubmed-82732772021-07-13 A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids Takeda, Haruna Front Genet Genetics Systematic approaches for functionally validating cancer genes are needed since numerous genes mutated in cancer tissues have been identified from cancer genome sequencing. The mouse organoid culture system has been extensively used in the field of cancer research since mouse organoids can faithfully recapitulate the physiological behavior of the cells. Taking advantage of this, we recently described a platform for functionally validating colorectal cancer (CRC) driver genes that utilized CRISPR-Cas9 in mouse intestinal tumor organoids. In this review, we will describe how mouse organoids have been applied to CRC research and focus on how CRC genes can be validated using mouse organoids. Frontiers Media S.A. 2021-06-28 /pmc/articles/PMC8273277/ /pubmed/34262603 http://dx.doi.org/10.3389/fgene.2021.698771 Text en Copyright © 2021 Takeda. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Takeda, Haruna
A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids
title A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids
title_full A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids
title_fullStr A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids
title_full_unstemmed A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids
title_short A Platform for Validating Colorectal Cancer Driver Genes Using Mouse Organoids
title_sort platform for validating colorectal cancer driver genes using mouse organoids
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273277/
https://www.ncbi.nlm.nih.gov/pubmed/34262603
http://dx.doi.org/10.3389/fgene.2021.698771
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