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Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures

The peritumoral regions of WHO grade II gliomas, like astrocytoma and oligodendroglioma, have been reported to show epileptiform activities. An imbalance of glutamatergic and GABAergic mechanisms is primarily responsible for the generation of epileptiform activities. Here we have compared the electr...

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Autores principales: Dey, Soumil, Doddamani, Ramesh Sharanappa, Banerjee Dixit, Aparna, Tripathi, Manjari, Sharma, Meher Chand, Chandra, P. Sarat, Banerjee, Jyotirmoy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273299/
https://www.ncbi.nlm.nih.gov/pubmed/34262432
http://dx.doi.org/10.3389/fnins.2021.689769
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author Dey, Soumil
Doddamani, Ramesh Sharanappa
Banerjee Dixit, Aparna
Tripathi, Manjari
Sharma, Meher Chand
Chandra, P. Sarat
Banerjee, Jyotirmoy
author_facet Dey, Soumil
Doddamani, Ramesh Sharanappa
Banerjee Dixit, Aparna
Tripathi, Manjari
Sharma, Meher Chand
Chandra, P. Sarat
Banerjee, Jyotirmoy
author_sort Dey, Soumil
collection PubMed
description The peritumoral regions of WHO grade II gliomas, like astrocytoma and oligodendroglioma, have been reported to show epileptiform activities. An imbalance of glutamatergic and GABAergic mechanisms is primarily responsible for the generation of epileptiform activities. Here we have compared the electrophysiological properties of pyramidal neurons in intraoperative peritumoral specimens obtained from glioma patients with (GS) and without (GN) a history of seizures at presentation. Histology and immunohistochemistry were performed to assess the infiltration of proliferating cells at the peritumoral tissues. Whole-cell patch clamp technique was performed to measure the spontaneous glutamatergic and GABAergic activity onto pyramidal neurons in the peritumoral samples of GS (n = 11) and GN (n = 15) patients. The cytoarchitecture of the peritumoral tissues was devoid of Ki67 immuno-positive cells. We observed a higher frequency of spontaneous glutamatergic and GABAergic activities onto pyramidal neurons of the peritumoral samples of GS patients. Our findings suggest that, in spite of similar histopathological features, the pyramidal neurons in the peritumoral samples of GS and GN patients showed differences in spontaneous excitatory and inhibitory synaptic neurotransmission. An alteration in postsynaptic currents may contribute to the spontaneous epileptiform activity in GS patients.
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spelling pubmed-82732992021-07-13 Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures Dey, Soumil Doddamani, Ramesh Sharanappa Banerjee Dixit, Aparna Tripathi, Manjari Sharma, Meher Chand Chandra, P. Sarat Banerjee, Jyotirmoy Front Neurosci Neuroscience The peritumoral regions of WHO grade II gliomas, like astrocytoma and oligodendroglioma, have been reported to show epileptiform activities. An imbalance of glutamatergic and GABAergic mechanisms is primarily responsible for the generation of epileptiform activities. Here we have compared the electrophysiological properties of pyramidal neurons in intraoperative peritumoral specimens obtained from glioma patients with (GS) and without (GN) a history of seizures at presentation. Histology and immunohistochemistry were performed to assess the infiltration of proliferating cells at the peritumoral tissues. Whole-cell patch clamp technique was performed to measure the spontaneous glutamatergic and GABAergic activity onto pyramidal neurons in the peritumoral samples of GS (n = 11) and GN (n = 15) patients. The cytoarchitecture of the peritumoral tissues was devoid of Ki67 immuno-positive cells. We observed a higher frequency of spontaneous glutamatergic and GABAergic activities onto pyramidal neurons of the peritumoral samples of GS patients. Our findings suggest that, in spite of similar histopathological features, the pyramidal neurons in the peritumoral samples of GS and GN patients showed differences in spontaneous excitatory and inhibitory synaptic neurotransmission. An alteration in postsynaptic currents may contribute to the spontaneous epileptiform activity in GS patients. Frontiers Media S.A. 2021-06-28 /pmc/articles/PMC8273299/ /pubmed/34262432 http://dx.doi.org/10.3389/fnins.2021.689769 Text en Copyright © 2021 Dey, Doddamani, Banerjee Dixit, Tripathi, Sharma, Chandra and Banerjee. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Dey, Soumil
Doddamani, Ramesh Sharanappa
Banerjee Dixit, Aparna
Tripathi, Manjari
Sharma, Meher Chand
Chandra, P. Sarat
Banerjee, Jyotirmoy
Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures
title Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures
title_full Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures
title_fullStr Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures
title_full_unstemmed Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures
title_short Altered Spontaneous Glutamatergic and GABAergic Activity in the Peritumoral Cortex of Low-Grade Gliomas Presenting With History of Seizures
title_sort altered spontaneous glutamatergic and gabaergic activity in the peritumoral cortex of low-grade gliomas presenting with history of seizures
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273299/
https://www.ncbi.nlm.nih.gov/pubmed/34262432
http://dx.doi.org/10.3389/fnins.2021.689769
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