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Cell cycle arrest of S. cerevisiae in conjunction with labeling of the cell wall
S. cerevisiae can be arrested in metaphase by depleting Cdc20. We describe (1) how to achieve this arrest and verify it, (2) how to label cell surface glycans covalently to distinguish mother from bud, and (3) how to detect the nucleolus and learn that it remains in the mother domain upon arrest. Fo...
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273410/ https://www.ncbi.nlm.nih.gov/pubmed/34286287 http://dx.doi.org/10.1016/j.xpro.2021.100646 |