Cargando…
Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells
Leydig cells (LC) are the main testicular androgen-producing cells. In eutherian mammals, two types of LCs emerge successively during testicular development, fetal Leydig cells (FLCs) and adult Leydig cells (ALCs). Both display significant differences in androgen production and regulation. Using bul...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273516/ https://www.ncbi.nlm.nih.gov/pubmed/34262907 http://dx.doi.org/10.3389/fcell.2021.695546 |
_version_ | 1783721385401516032 |
---|---|
author | Sararols, Pauline Stévant, Isabelle Neirijnck, Yasmine Rebourcet, Diane Darbey, Annalucia Curley, Michael K. Kühne, Françoise Dermitzakis, Emmanouil Smith, Lee B. Nef, Serge |
author_facet | Sararols, Pauline Stévant, Isabelle Neirijnck, Yasmine Rebourcet, Diane Darbey, Annalucia Curley, Michael K. Kühne, Françoise Dermitzakis, Emmanouil Smith, Lee B. Nef, Serge |
author_sort | Sararols, Pauline |
collection | PubMed |
description | Leydig cells (LC) are the main testicular androgen-producing cells. In eutherian mammals, two types of LCs emerge successively during testicular development, fetal Leydig cells (FLCs) and adult Leydig cells (ALCs). Both display significant differences in androgen production and regulation. Using bulk RNA sequencing, we compared the transcriptomes of both LC populations to characterize their specific transcriptional and functional features. Despite similar transcriptomic profiles, a quarter of the genes show significant variations in expression between FLCs and ALCs. Non-transcriptional events, such as alternative splicing was also observed, including a high rate of intron retention in FLCs compared to ALCs. The use of single-cell RNA sequencing data also allowed the identification of nine FLC-specific genes and 50 ALC-specific genes. Expression of the corticotropin-releasing hormone 1 (Crhr1) receptor and the ACTH receptor melanocortin type 2 receptor (Mc2r) specifically in FLCs suggests a dual regulation of steroidogenesis. The androstenedione synthesis by FLCs is stimulated by luteinizing hormone (LH), corticotrophin-releasing hormone (CRH), and adrenocorticotropic hormone (ACTH) whereas the testosterone synthesis by ALCs is dependent exclusively on LH. Overall, our study provides a useful database to explore LC development and functions. |
format | Online Article Text |
id | pubmed-8273516 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82735162021-07-13 Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells Sararols, Pauline Stévant, Isabelle Neirijnck, Yasmine Rebourcet, Diane Darbey, Annalucia Curley, Michael K. Kühne, Françoise Dermitzakis, Emmanouil Smith, Lee B. Nef, Serge Front Cell Dev Biol Cell and Developmental Biology Leydig cells (LC) are the main testicular androgen-producing cells. In eutherian mammals, two types of LCs emerge successively during testicular development, fetal Leydig cells (FLCs) and adult Leydig cells (ALCs). Both display significant differences in androgen production and regulation. Using bulk RNA sequencing, we compared the transcriptomes of both LC populations to characterize their specific transcriptional and functional features. Despite similar transcriptomic profiles, a quarter of the genes show significant variations in expression between FLCs and ALCs. Non-transcriptional events, such as alternative splicing was also observed, including a high rate of intron retention in FLCs compared to ALCs. The use of single-cell RNA sequencing data also allowed the identification of nine FLC-specific genes and 50 ALC-specific genes. Expression of the corticotropin-releasing hormone 1 (Crhr1) receptor and the ACTH receptor melanocortin type 2 receptor (Mc2r) specifically in FLCs suggests a dual regulation of steroidogenesis. The androstenedione synthesis by FLCs is stimulated by luteinizing hormone (LH), corticotrophin-releasing hormone (CRH), and adrenocorticotropic hormone (ACTH) whereas the testosterone synthesis by ALCs is dependent exclusively on LH. Overall, our study provides a useful database to explore LC development and functions. Frontiers Media S.A. 2021-06-28 /pmc/articles/PMC8273516/ /pubmed/34262907 http://dx.doi.org/10.3389/fcell.2021.695546 Text en Copyright © 2021 Sararols, Stévant, Neirijnck, Rebourcet, Darbey, Curley, Kühne, Dermitzakis, Smith and Nef. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Sararols, Pauline Stévant, Isabelle Neirijnck, Yasmine Rebourcet, Diane Darbey, Annalucia Curley, Michael K. Kühne, Françoise Dermitzakis, Emmanouil Smith, Lee B. Nef, Serge Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells |
title | Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells |
title_full | Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells |
title_fullStr | Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells |
title_full_unstemmed | Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells |
title_short | Specific Transcriptomic Signatures and Dual Regulation of Steroidogenesis Between Fetal and Adult Mouse Leydig Cells |
title_sort | specific transcriptomic signatures and dual regulation of steroidogenesis between fetal and adult mouse leydig cells |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273516/ https://www.ncbi.nlm.nih.gov/pubmed/34262907 http://dx.doi.org/10.3389/fcell.2021.695546 |
work_keys_str_mv | AT sararolspauline specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT stevantisabelle specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT neirijnckyasmine specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT rebourcetdiane specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT darbeyannalucia specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT curleymichaelk specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT kuhnefrancoise specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT dermitzakisemmanouil specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT smithleeb specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells AT nefserge specifictranscriptomicsignaturesanddualregulationofsteroidogenesisbetweenfetalandadultmouseleydigcells |