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Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation
BACKGROUND/AIMS: Bacteria-derived outer membrane vesicles (OMVs) are commonly associated with various biological activities and functions. Helicobacter pylori-derived OMVs are thought to contribute to pathogenesis. This study aimed to investigate the effects of H. pylori-derived OMVs. METHODS: H. py...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Korean Association of Internal Medicine
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273812/ https://www.ncbi.nlm.nih.gov/pubmed/33242939 http://dx.doi.org/10.3904/kjim.2019.432 |
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author | Choi, Mun Sun Ze, Eun Young Park, Jae Yong Shin, Tae-Seop Kim, Jae Gyu |
author_facet | Choi, Mun Sun Ze, Eun Young Park, Jae Yong Shin, Tae-Seop Kim, Jae Gyu |
author_sort | Choi, Mun Sun |
collection | PubMed |
description | BACKGROUND/AIMS: Bacteria-derived outer membrane vesicles (OMVs) are commonly associated with various biological activities and functions. Helicobacter pylori-derived OMVs are thought to contribute to pathogenesis. This study aimed to investigate the effects of H. pylori-derived OMVs. METHODS: H. pylori strains were isolated from patients with gastritis, gastric ulcer, or gastric cancer using endoscopic biopsy. The U-937, AGS, and MKN-45 cell lines were exposed to H. pylori and H. pylori-derived OMVs. The expression of interleukin 8 (IL-8) messenger RNA (mRNA) was assessed using reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR, and IL-8 secretion was analyzed using enzyme-linked immunosorbent assay. Nuclear factor kappa B (NF-κB) activation was evaluated by Western blotting. RESULTS: H. pylori and H. pylori-derived OMVs induced the expression of IL-8 mRNA and protein. Importantly, the bacteria induced higher IL-8 mRNA and protein expression than the OMVs. IL-8 expression was induced to different levels in response to H. pylori-derived OMVs from hosts with different gastric diseases. Western blotting revealed the increased phosphorylation and reduced degradation of inhibitor of NF-κB alpha in cells exposed to OMVs. CONCLUSIONS: H. pylori-derived OMVs may aid the development of various gastric diseases by inducing IL-8 production and NF-κB activation. |
format | Online Article Text |
id | pubmed-8273812 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-82738122021-07-20 Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation Choi, Mun Sun Ze, Eun Young Park, Jae Yong Shin, Tae-Seop Kim, Jae Gyu Korean J Intern Med Original Article BACKGROUND/AIMS: Bacteria-derived outer membrane vesicles (OMVs) are commonly associated with various biological activities and functions. Helicobacter pylori-derived OMVs are thought to contribute to pathogenesis. This study aimed to investigate the effects of H. pylori-derived OMVs. METHODS: H. pylori strains were isolated from patients with gastritis, gastric ulcer, or gastric cancer using endoscopic biopsy. The U-937, AGS, and MKN-45 cell lines were exposed to H. pylori and H. pylori-derived OMVs. The expression of interleukin 8 (IL-8) messenger RNA (mRNA) was assessed using reverse transcription-polymerase chain reaction (RT-PCR) and real-time RT-PCR, and IL-8 secretion was analyzed using enzyme-linked immunosorbent assay. Nuclear factor kappa B (NF-κB) activation was evaluated by Western blotting. RESULTS: H. pylori and H. pylori-derived OMVs induced the expression of IL-8 mRNA and protein. Importantly, the bacteria induced higher IL-8 mRNA and protein expression than the OMVs. IL-8 expression was induced to different levels in response to H. pylori-derived OMVs from hosts with different gastric diseases. Western blotting revealed the increased phosphorylation and reduced degradation of inhibitor of NF-κB alpha in cells exposed to OMVs. CONCLUSIONS: H. pylori-derived OMVs may aid the development of various gastric diseases by inducing IL-8 production and NF-κB activation. Korean Association of Internal Medicine 2021-07 2020-12-01 /pmc/articles/PMC8273812/ /pubmed/33242939 http://dx.doi.org/10.3904/kjim.2019.432 Text en Copyright © 2021 The Korean Association of Internal Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Choi, Mun Sun Ze, Eun Young Park, Jae Yong Shin, Tae-Seop Kim, Jae Gyu Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation |
title | Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation |
title_full | Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation |
title_fullStr | Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation |
title_full_unstemmed | Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation |
title_short | Helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa B activation |
title_sort | helicobacter pylori-derived outer membrane vesicles stimulate interleukin 8 secretion through nuclear factor kappa b activation |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273812/ https://www.ncbi.nlm.nih.gov/pubmed/33242939 http://dx.doi.org/10.3904/kjim.2019.432 |
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