Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
BACKGROUND/AIMS: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a major regulator of Wnt signaling, which is involved in fibroblast dysfunction. Because its role has not been evaluated in idiopathic pulmonary fibrosis (IPF), we examined the clinical implications of ROR2 expression. METHOD...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Korean Association of Internal Medicine
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273837/ https://www.ncbi.nlm.nih.gov/pubmed/32951408 http://dx.doi.org/10.3904/kjim.2019.270 |
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author | Son, Ji-Hye Lee, Jong-Uk Chin, Susie Go, Eun-Suk Park, Jai-Seong Shin, Hwa-Kyun Chang, Hun Soo Park, Jong-Sook Park, Choon-Sik |
author_facet | Son, Ji-Hye Lee, Jong-Uk Chin, Susie Go, Eun-Suk Park, Jai-Seong Shin, Hwa-Kyun Chang, Hun Soo Park, Jong-Sook Park, Choon-Sik |
author_sort | Son, Ji-Hye |
collection | PubMed |
description | BACKGROUND/AIMS: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a major regulator of Wnt signaling, which is involved in fibroblast dysfunction. Because its role has not been evaluated in idiopathic pulmonary fibrosis (IPF), we examined the clinical implications of ROR2 expression. METHODS: ROR2 mRNA expression was measured using reverse transcription polymerase chain reaction in lung tissue-derived fibroblasts from IPF patients (n = 14) and from controls (n = 10). ROR2 protein was measured using enzyme-linked immunosorbent assay in primary fibroblasts from IPF patients (n = 14) and controls (n = 10), and in bronchoalveolar lavage (BAL) fluids obtained from normal controls (NC; n = 30). IPF patients (n = 84), and other patients with interstitial lung diseases, including nonspecific interstitial pneumonia (NSIP; n = 10), hypersensitivity pneumonitis (HP; n = 10), and sarcoidosis (n = 10). RESULTS: ROR2 mRNA and protein levels were significantly higher in IPF fibroblasts than in controls (p = 0.003, p = 0.0017, respectively). ROR2 protein levels in BAL fluids from patients with IPF were significantly higher than in those from NC (p < 0.001), and from patients with NSIP (p = 0.006), HP (p = 0.004), or sarcoidosis (p = 0.004). Receiver operating characteristic curves showed a clear difference between IPF and NC in ROR2 protein level (area under the curve, 0.890; confidence interval, 0.829 to 0.950; p < 0.001). ROR2 protein levels were significantly higher in GAP stage III than in GAP stages I and II (p = 0.016). CONCLUSIONS: ROR2 may be related to the development of IPF, and its protein level may be a useful and severity-dependent candidate marker for IPF. |
format | Online Article Text |
id | pubmed-8273837 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | The Korean Association of Internal Medicine |
record_format | MEDLINE/PubMed |
spelling | pubmed-82738372021-07-20 Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis Son, Ji-Hye Lee, Jong-Uk Chin, Susie Go, Eun-Suk Park, Jai-Seong Shin, Hwa-Kyun Chang, Hun Soo Park, Jong-Sook Park, Choon-Sik Korean J Intern Med Original Article BACKGROUND/AIMS: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a major regulator of Wnt signaling, which is involved in fibroblast dysfunction. Because its role has not been evaluated in idiopathic pulmonary fibrosis (IPF), we examined the clinical implications of ROR2 expression. METHODS: ROR2 mRNA expression was measured using reverse transcription polymerase chain reaction in lung tissue-derived fibroblasts from IPF patients (n = 14) and from controls (n = 10). ROR2 protein was measured using enzyme-linked immunosorbent assay in primary fibroblasts from IPF patients (n = 14) and controls (n = 10), and in bronchoalveolar lavage (BAL) fluids obtained from normal controls (NC; n = 30). IPF patients (n = 84), and other patients with interstitial lung diseases, including nonspecific interstitial pneumonia (NSIP; n = 10), hypersensitivity pneumonitis (HP; n = 10), and sarcoidosis (n = 10). RESULTS: ROR2 mRNA and protein levels were significantly higher in IPF fibroblasts than in controls (p = 0.003, p = 0.0017, respectively). ROR2 protein levels in BAL fluids from patients with IPF were significantly higher than in those from NC (p < 0.001), and from patients with NSIP (p = 0.006), HP (p = 0.004), or sarcoidosis (p = 0.004). Receiver operating characteristic curves showed a clear difference between IPF and NC in ROR2 protein level (area under the curve, 0.890; confidence interval, 0.829 to 0.950; p < 0.001). ROR2 protein levels were significantly higher in GAP stage III than in GAP stages I and II (p = 0.016). CONCLUSIONS: ROR2 may be related to the development of IPF, and its protein level may be a useful and severity-dependent candidate marker for IPF. The Korean Association of Internal Medicine 2021-07 2020-09-21 /pmc/articles/PMC8273837/ /pubmed/32951408 http://dx.doi.org/10.3904/kjim.2019.270 Text en Copyright © 2021 The Korean Association of Internal Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Son, Ji-Hye Lee, Jong-Uk Chin, Susie Go, Eun-Suk Park, Jai-Seong Shin, Hwa-Kyun Chang, Hun Soo Park, Jong-Sook Park, Choon-Sik Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis |
title | Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis |
title_full | Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis |
title_fullStr | Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis |
title_full_unstemmed | Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis |
title_short | Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis |
title_sort | upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273837/ https://www.ncbi.nlm.nih.gov/pubmed/32951408 http://dx.doi.org/10.3904/kjim.2019.270 |
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