Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis

BACKGROUND/AIMS: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a major regulator of Wnt signaling, which is involved in fibroblast dysfunction. Because its role has not been evaluated in idiopathic pulmonary fibrosis (IPF), we examined the clinical implications of ROR2 expression. METHOD...

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Autores principales: Son, Ji-Hye, Lee, Jong-Uk, Chin, Susie, Go, Eun-Suk, Park, Jai-Seong, Shin, Hwa-Kyun, Chang, Hun Soo, Park, Jong-Sook, Park, Choon-Sik
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Association of Internal Medicine 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273837/
https://www.ncbi.nlm.nih.gov/pubmed/32951408
http://dx.doi.org/10.3904/kjim.2019.270
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author Son, Ji-Hye
Lee, Jong-Uk
Chin, Susie
Go, Eun-Suk
Park, Jai-Seong
Shin, Hwa-Kyun
Chang, Hun Soo
Park, Jong-Sook
Park, Choon-Sik
author_facet Son, Ji-Hye
Lee, Jong-Uk
Chin, Susie
Go, Eun-Suk
Park, Jai-Seong
Shin, Hwa-Kyun
Chang, Hun Soo
Park, Jong-Sook
Park, Choon-Sik
author_sort Son, Ji-Hye
collection PubMed
description BACKGROUND/AIMS: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a major regulator of Wnt signaling, which is involved in fibroblast dysfunction. Because its role has not been evaluated in idiopathic pulmonary fibrosis (IPF), we examined the clinical implications of ROR2 expression. METHODS: ROR2 mRNA expression was measured using reverse transcription polymerase chain reaction in lung tissue-derived fibroblasts from IPF patients (n = 14) and from controls (n = 10). ROR2 protein was measured using enzyme-linked immunosorbent assay in primary fibroblasts from IPF patients (n = 14) and controls (n = 10), and in bronchoalveolar lavage (BAL) fluids obtained from normal controls (NC; n = 30). IPF patients (n = 84), and other patients with interstitial lung diseases, including nonspecific interstitial pneumonia (NSIP; n = 10), hypersensitivity pneumonitis (HP; n = 10), and sarcoidosis (n = 10). RESULTS: ROR2 mRNA and protein levels were significantly higher in IPF fibroblasts than in controls (p = 0.003, p = 0.0017, respectively). ROR2 protein levels in BAL fluids from patients with IPF were significantly higher than in those from NC (p < 0.001), and from patients with NSIP (p = 0.006), HP (p = 0.004), or sarcoidosis (p = 0.004). Receiver operating characteristic curves showed a clear difference between IPF and NC in ROR2 protein level (area under the curve, 0.890; confidence interval, 0.829 to 0.950; p < 0.001). ROR2 protein levels were significantly higher in GAP stage III than in GAP stages I and II (p = 0.016). CONCLUSIONS: ROR2 may be related to the development of IPF, and its protein level may be a useful and severity-dependent candidate marker for IPF.
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spelling pubmed-82738372021-07-20 Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis Son, Ji-Hye Lee, Jong-Uk Chin, Susie Go, Eun-Suk Park, Jai-Seong Shin, Hwa-Kyun Chang, Hun Soo Park, Jong-Sook Park, Choon-Sik Korean J Intern Med Original Article BACKGROUND/AIMS: Receptor tyrosine kinase-like orphan receptor 2 (ROR2) is a major regulator of Wnt signaling, which is involved in fibroblast dysfunction. Because its role has not been evaluated in idiopathic pulmonary fibrosis (IPF), we examined the clinical implications of ROR2 expression. METHODS: ROR2 mRNA expression was measured using reverse transcription polymerase chain reaction in lung tissue-derived fibroblasts from IPF patients (n = 14) and from controls (n = 10). ROR2 protein was measured using enzyme-linked immunosorbent assay in primary fibroblasts from IPF patients (n = 14) and controls (n = 10), and in bronchoalveolar lavage (BAL) fluids obtained from normal controls (NC; n = 30). IPF patients (n = 84), and other patients with interstitial lung diseases, including nonspecific interstitial pneumonia (NSIP; n = 10), hypersensitivity pneumonitis (HP; n = 10), and sarcoidosis (n = 10). RESULTS: ROR2 mRNA and protein levels were significantly higher in IPF fibroblasts than in controls (p = 0.003, p = 0.0017, respectively). ROR2 protein levels in BAL fluids from patients with IPF were significantly higher than in those from NC (p < 0.001), and from patients with NSIP (p = 0.006), HP (p = 0.004), or sarcoidosis (p = 0.004). Receiver operating characteristic curves showed a clear difference between IPF and NC in ROR2 protein level (area under the curve, 0.890; confidence interval, 0.829 to 0.950; p < 0.001). ROR2 protein levels were significantly higher in GAP stage III than in GAP stages I and II (p = 0.016). CONCLUSIONS: ROR2 may be related to the development of IPF, and its protein level may be a useful and severity-dependent candidate marker for IPF. The Korean Association of Internal Medicine 2021-07 2020-09-21 /pmc/articles/PMC8273837/ /pubmed/32951408 http://dx.doi.org/10.3904/kjim.2019.270 Text en Copyright © 2021 The Korean Association of Internal Medicine https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Son, Ji-Hye
Lee, Jong-Uk
Chin, Susie
Go, Eun-Suk
Park, Jai-Seong
Shin, Hwa-Kyun
Chang, Hun Soo
Park, Jong-Sook
Park, Choon-Sik
Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
title Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
title_full Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
title_fullStr Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
title_full_unstemmed Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
title_short Upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
title_sort upregulation of receptor tyrosine kinase-like orphan receptor 2 in idiopathic pulmonary fibrosis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8273837/
https://www.ncbi.nlm.nih.gov/pubmed/32951408
http://dx.doi.org/10.3904/kjim.2019.270
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