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Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma

BACKGROUND: The long noncoding RNA (lncRNA) GAPLINC, or gastric adenocarcinoma predictive long intergenic ncRNA, plays a carcinogenic role in a variety of different tumor types. There is limited information regarding the biological function of GAPLINC in the development of esophageal squamous cell c...

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Autores principales: Wang, Peng, Yang, Song, Dai, Shengbin, Ni, Qingtao, Liu, Huilan, Yu, Lei, Lu, Kaijin, Han, Gaohua, Huang, Junxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274232/
https://www.ncbi.nlm.nih.gov/pubmed/34262290
http://dx.doi.org/10.2147/OTT.S299394
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author Wang, Peng
Yang, Song
Dai, Shengbin
Ni, Qingtao
Liu, Huilan
Yu, Lei
Lu, Kaijin
Han, Gaohua
Huang, Junxing
author_facet Wang, Peng
Yang, Song
Dai, Shengbin
Ni, Qingtao
Liu, Huilan
Yu, Lei
Lu, Kaijin
Han, Gaohua
Huang, Junxing
author_sort Wang, Peng
collection PubMed
description BACKGROUND: The long noncoding RNA (lncRNA) GAPLINC, or gastric adenocarcinoma predictive long intergenic ncRNA, plays a carcinogenic role in a variety of different tumor types. There is limited information regarding the biological function of GAPLINC in the development of esophageal squamous cell carcinoma (ESCC). METHODS: Surgical tissue samples of 40 patients undergoing ESCC radical surgery were collected, including ESCC tissues and corresponding adjacent normal tissues. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of lncRNA GAPLINC in the human ESCC cell line (TE11). The function role of LncRNA GAPLINC was detected after specific siRNA interference and overexpression in the TE11 cell line. The effects of LncRNA GAPLINC on ESCC cell proliferation, migration and invasion abilities were investigated by flow cytometry, using the Cell Counting Kit-8 (CCK-8), and by Transwell migration assays, respectively. RESULTS: The expression of lncRNA GAPLINC in ESCC tissues was significantly higher than that in corresponding adjacent normal tissues (P<0.05) and correlated with the degree of tumor differentiation (P<0.05). Compared with human esophageal normal epithelial cell lines, the expression of LncRNA GAPLINC was significantly higher in the human ESCC cell line (P<0.05). CCK-8 assays showed that LncRNA GAPLINC overexpression increased the growth rate of cells (P<0.05). Transwell experiments showed that LncRNA GAPLINC overexpression increased the ability of cell migration and invasion compared to control cells (P<0.05). Annexin V assay revealed that LncRNA GAPLINC silencing increased early stage apoptosis (P<0. 05). CONCLUSION: Our results suggest that LncRNA GAPLINC may be used as a biomarker for the diagnosis and monitoring of ESCC, and may play an oncogenic role in ESCC.
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spelling pubmed-82742322021-07-13 Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma Wang, Peng Yang, Song Dai, Shengbin Ni, Qingtao Liu, Huilan Yu, Lei Lu, Kaijin Han, Gaohua Huang, Junxing Onco Targets Ther Original Research BACKGROUND: The long noncoding RNA (lncRNA) GAPLINC, or gastric adenocarcinoma predictive long intergenic ncRNA, plays a carcinogenic role in a variety of different tumor types. There is limited information regarding the biological function of GAPLINC in the development of esophageal squamous cell carcinoma (ESCC). METHODS: Surgical tissue samples of 40 patients undergoing ESCC radical surgery were collected, including ESCC tissues and corresponding adjacent normal tissues. Quantitative real-time PCR (qRT-PCR) was used to detect the expression of lncRNA GAPLINC in the human ESCC cell line (TE11). The function role of LncRNA GAPLINC was detected after specific siRNA interference and overexpression in the TE11 cell line. The effects of LncRNA GAPLINC on ESCC cell proliferation, migration and invasion abilities were investigated by flow cytometry, using the Cell Counting Kit-8 (CCK-8), and by Transwell migration assays, respectively. RESULTS: The expression of lncRNA GAPLINC in ESCC tissues was significantly higher than that in corresponding adjacent normal tissues (P<0.05) and correlated with the degree of tumor differentiation (P<0.05). Compared with human esophageal normal epithelial cell lines, the expression of LncRNA GAPLINC was significantly higher in the human ESCC cell line (P<0.05). CCK-8 assays showed that LncRNA GAPLINC overexpression increased the growth rate of cells (P<0.05). Transwell experiments showed that LncRNA GAPLINC overexpression increased the ability of cell migration and invasion compared to control cells (P<0.05). Annexin V assay revealed that LncRNA GAPLINC silencing increased early stage apoptosis (P<0. 05). CONCLUSION: Our results suggest that LncRNA GAPLINC may be used as a biomarker for the diagnosis and monitoring of ESCC, and may play an oncogenic role in ESCC. Dove 2021-07-05 /pmc/articles/PMC8274232/ /pubmed/34262290 http://dx.doi.org/10.2147/OTT.S299394 Text en © 2021 Wang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).
spellingShingle Original Research
Wang, Peng
Yang, Song
Dai, Shengbin
Ni, Qingtao
Liu, Huilan
Yu, Lei
Lu, Kaijin
Han, Gaohua
Huang, Junxing
Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma
title Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma
title_full Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma
title_fullStr Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma
title_full_unstemmed Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma
title_short Expression and Clinical Value of LncRNA GAPLINC in Esophageal Squamous Cell Carcinoma
title_sort expression and clinical value of lncrna gaplinc in esophageal squamous cell carcinoma
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274232/
https://www.ncbi.nlm.nih.gov/pubmed/34262290
http://dx.doi.org/10.2147/OTT.S299394
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