Cargando…

Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt

We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay (ELISA). The slide plate agglutinati...

Descripción completa

Detalles Bibliográficos
Autores principales: El-Ashram, Saeed, Hashad, Mahmoud E., Abdel-Alim, G. A., Abdelhamid, Taher, N. Deif, Heba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274858/
https://www.ncbi.nlm.nih.gov/pubmed/34252126
http://dx.doi.org/10.1371/journal.pone.0254220
_version_ 1783721617573019648
author El-Ashram, Saeed
Hashad, Mahmoud E.
Abdel-Alim, G. A.
Abdelhamid, Taher
N. Deif, Heba
author_facet El-Ashram, Saeed
Hashad, Mahmoud E.
Abdel-Alim, G. A.
Abdelhamid, Taher
N. Deif, Heba
author_sort El-Ashram, Saeed
collection PubMed
description We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay (ELISA). The slide plate agglutination examination, a serological test, indicated the prevalence of Mg and Ms infections of 10.9% and 13.2%, respectively. On 91 SPA test positive serum samples for either Mg or Ms, a passive hemagglutination/hemagglutination inhibition (HI) test was performed. The SPA and HI test findings were found to be comparable. On 90 SPA test positive samples, an ELISA was performed using commercial kits for Mg and Ms serodiagnosis. According to the ELISA data, only 83.33% and 18.88% of SPA test positive samples were confirmed as positive for Ms and Mg infections, respectively. The prevalence increased to 84.44% and 77.77%, respectively, when suspected samples were deemed positive.
format Online
Article
Text
id pubmed-8274858
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-82748582021-07-27 Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt El-Ashram, Saeed Hashad, Mahmoud E. Abdel-Alim, G. A. Abdelhamid, Taher N. Deif, Heba PLoS One Research Article We aimed to investigate Mycoplasma infections among chicken flocks (Ross, Lohmann and native) in Giza, Egypt, using serological tests, including the slide plate agglutination (SPA) test, hemagglutination inhibition (HI) test, and enzyme-linked immunosorbent assay (ELISA). The slide plate agglutination examination, a serological test, indicated the prevalence of Mg and Ms infections of 10.9% and 13.2%, respectively. On 91 SPA test positive serum samples for either Mg or Ms, a passive hemagglutination/hemagglutination inhibition (HI) test was performed. The SPA and HI test findings were found to be comparable. On 90 SPA test positive samples, an ELISA was performed using commercial kits for Mg and Ms serodiagnosis. According to the ELISA data, only 83.33% and 18.88% of SPA test positive samples were confirmed as positive for Ms and Mg infections, respectively. The prevalence increased to 84.44% and 77.77%, respectively, when suspected samples were deemed positive. Public Library of Science 2021-07-12 /pmc/articles/PMC8274858/ /pubmed/34252126 http://dx.doi.org/10.1371/journal.pone.0254220 Text en © 2021 El-Ashram et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
El-Ashram, Saeed
Hashad, Mahmoud E.
Abdel-Alim, G. A.
Abdelhamid, Taher
N. Deif, Heba
Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt
title Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt
title_full Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt
title_fullStr Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt
title_full_unstemmed Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt
title_short Seroprevalence of mycoplasmosis in broiler, layer, and native chickens in Giza, Egypt
title_sort seroprevalence of mycoplasmosis in broiler, layer, and native chickens in giza, egypt
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8274858/
https://www.ncbi.nlm.nih.gov/pubmed/34252126
http://dx.doi.org/10.1371/journal.pone.0254220
work_keys_str_mv AT elashramsaeed seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt
AT hashadmahmoude seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt
AT abdelalimga seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt
AT abdelhamidtaher seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt
AT ndeifheba seroprevalenceofmycoplasmosisinbroilerlayerandnativechickensingizaegypt