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Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy
PURPOSE: Hypoxia is considered to be obstructive to tumor treatment, but the reduced oxygen surroundings provide a suitable habitat for Bifidobacterium bifidum (BF) to colonize. The anaerobe BF selectively colonizes into tumors following systemic injection due to its preference for the hypoxia in th...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275162/ https://www.ncbi.nlm.nih.gov/pubmed/34267516 http://dx.doi.org/10.2147/IJN.S315650 |
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author | Tang, Yu Chen, Chun Jiang, Binglei Wang, Lu Jiang, Fujie Wang, Disen Wang, Yaotai Yang, Haiyan Ou, Xia Du, Yan Wang, Qi Zou, Jianzhong |
author_facet | Tang, Yu Chen, Chun Jiang, Binglei Wang, Lu Jiang, Fujie Wang, Disen Wang, Yaotai Yang, Haiyan Ou, Xia Du, Yan Wang, Qi Zou, Jianzhong |
author_sort | Tang, Yu |
collection | PubMed |
description | PURPOSE: Hypoxia is considered to be obstructive to tumor treatment, but the reduced oxygen surroundings provide a suitable habitat for Bifidobacterium bifidum (BF) to colonize. The anaerobe BF selectively colonizes into tumors following systemic injection due to its preference for the hypoxia in the tumor cores. Therefore, BF may be a potential targeting agent which could be used effectively in tumor treatment. We aimed to determine whether a novel BF-mediated strategy, that was designed to deliver AP-PFH/PLGA NPs (aptamers CCFM641-5-functionalized Perfluorohexane (PFH) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles) by aptamer-directed approach into solid tumor based on the tumor-targeting ability of BF, could improve efficiency of high intensity focused ultrasound (HIFU) treatment of breast cancer. METHODS: We synthesized AP-PFH/PLGA NPs using double emulsion method and carbodiimide method. Then, we evaluated targeting ability of AP-PFH/PLGA NPs to BF in vivo. Finally, we studied the efficacy of HIFU ablation based on BF plus AP-PFH/PLGA NPs (BF-mediated HIFU ablation) in tumor. RESULTS: The elaborately designed AP-PFH/PLGA NPs can target BF colonized in tumor to achieve high tumor accumulation, which can significantly enhance HIFU therapeutic efficiency. We also found that, compared with traditional chemotherapy, this therapy not only inhibits tumor growth, but also significantly prolongs the survival time of mice. More importantly, this treatment strategy has no obvious side effects. CONCLUSION: We successfully established a novel therapy method, BF-mediated HIFU ablation, which provides an excellent platform for highly efficient and non-invasive therapy of tumor. |
format | Online Article Text |
id | pubmed-8275162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-82751622021-07-14 Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy Tang, Yu Chen, Chun Jiang, Binglei Wang, Lu Jiang, Fujie Wang, Disen Wang, Yaotai Yang, Haiyan Ou, Xia Du, Yan Wang, Qi Zou, Jianzhong Int J Nanomedicine Original Research PURPOSE: Hypoxia is considered to be obstructive to tumor treatment, but the reduced oxygen surroundings provide a suitable habitat for Bifidobacterium bifidum (BF) to colonize. The anaerobe BF selectively colonizes into tumors following systemic injection due to its preference for the hypoxia in the tumor cores. Therefore, BF may be a potential targeting agent which could be used effectively in tumor treatment. We aimed to determine whether a novel BF-mediated strategy, that was designed to deliver AP-PFH/PLGA NPs (aptamers CCFM641-5-functionalized Perfluorohexane (PFH) loaded poly(lactic-co-glycolic acid) (PLGA) nanoparticles) by aptamer-directed approach into solid tumor based on the tumor-targeting ability of BF, could improve efficiency of high intensity focused ultrasound (HIFU) treatment of breast cancer. METHODS: We synthesized AP-PFH/PLGA NPs using double emulsion method and carbodiimide method. Then, we evaluated targeting ability of AP-PFH/PLGA NPs to BF in vivo. Finally, we studied the efficacy of HIFU ablation based on BF plus AP-PFH/PLGA NPs (BF-mediated HIFU ablation) in tumor. RESULTS: The elaborately designed AP-PFH/PLGA NPs can target BF colonized in tumor to achieve high tumor accumulation, which can significantly enhance HIFU therapeutic efficiency. We also found that, compared with traditional chemotherapy, this therapy not only inhibits tumor growth, but also significantly prolongs the survival time of mice. More importantly, this treatment strategy has no obvious side effects. CONCLUSION: We successfully established a novel therapy method, BF-mediated HIFU ablation, which provides an excellent platform for highly efficient and non-invasive therapy of tumor. Dove 2021-07-06 /pmc/articles/PMC8275162/ /pubmed/34267516 http://dx.doi.org/10.2147/IJN.S315650 Text en © 2021 Tang et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Tang, Yu Chen, Chun Jiang, Binglei Wang, Lu Jiang, Fujie Wang, Disen Wang, Yaotai Yang, Haiyan Ou, Xia Du, Yan Wang, Qi Zou, Jianzhong Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy |
title | Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy |
title_full | Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy |
title_fullStr | Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy |
title_full_unstemmed | Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy |
title_short | Bifidobacterium bifidum-Mediated Specific Delivery of Nanoparticles for Tumor Therapy |
title_sort | bifidobacterium bifidum-mediated specific delivery of nanoparticles for tumor therapy |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275162/ https://www.ncbi.nlm.nih.gov/pubmed/34267516 http://dx.doi.org/10.2147/IJN.S315650 |
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