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Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers
This research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotte...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275643/ https://www.ncbi.nlm.nih.gov/pubmed/34268345 http://dx.doi.org/10.3389/fvets.2021.663577 |
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author | Saad, Adel Hassan Ahmed, Mohamed S. Aboubakr, Mohamed Ghoneim, Hanan A. Abdel-Daim, Mohamed M. Albadrani, Ghadeer M. Arafat, Nagah Fadl, Sabreen Ezzat Abdo, Walied |
author_facet | Saad, Adel Hassan Ahmed, Mohamed S. Aboubakr, Mohamed Ghoneim, Hanan A. Abdel-Daim, Mohamed M. Albadrani, Ghadeer M. Arafat, Nagah Fadl, Sabreen Ezzat Abdo, Walied |
author_sort | Saad, Adel Hassan |
collection | PubMed |
description | This research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotted into six groups according to HS and/or Ruminococcus with or without enzyme supplementation. The first group was the control one, with a formulated diet and normal environmental temperature but without any supplement. The second group fed on Ruminococcus-supplemented diet (1 kg/kg diet). The third group fed on a formulated diet without supplement, and Ruminococcus and digestive enzymes were given in drinking water (0.1 ml/L). The fourth one was the heat stress group, with a normal formulated diet. The fifth and the sixth groups served as second and third groups, respectively, but with heat stress. The results of this experiment indicated that thermal temperature negatively affected the parameters of growth performance, serum biochemical, tissue antioxidants, and phagocytic assay. Moreover, heat stress led to pathological lesions in the internal organs and affected the expression of some genes related to heat stress, including proapoptotic genes such as caspase8 and bax, inflammatory genes such as NF-κβ1, and heat shock protein such as HSP 70 in the bursal tissue. These bad effects and abnormalities were mitigated by Ruminococcus alone or with enzyme supplementation, which improved all the above-mentioned parameters. |
format | Online Article Text |
id | pubmed-8275643 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-82756432021-07-14 Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers Saad, Adel Hassan Ahmed, Mohamed S. Aboubakr, Mohamed Ghoneim, Hanan A. Abdel-Daim, Mohamed M. Albadrani, Ghadeer M. Arafat, Nagah Fadl, Sabreen Ezzat Abdo, Walied Front Vet Sci Veterinary Science This research was conducted to evaluate the impact of dietary or drinking water Ruminococcus sp. supplementation and/or heat stress (HS) on the growth, serum biochemistry, tissue antioxidant, phagocytic assay, histopathology, and bursa gene expression of broilers. Day-old broiler chicks were allotted into six groups according to HS and/or Ruminococcus with or without enzyme supplementation. The first group was the control one, with a formulated diet and normal environmental temperature but without any supplement. The second group fed on Ruminococcus-supplemented diet (1 kg/kg diet). The third group fed on a formulated diet without supplement, and Ruminococcus and digestive enzymes were given in drinking water (0.1 ml/L). The fourth one was the heat stress group, with a normal formulated diet. The fifth and the sixth groups served as second and third groups, respectively, but with heat stress. The results of this experiment indicated that thermal temperature negatively affected the parameters of growth performance, serum biochemical, tissue antioxidants, and phagocytic assay. Moreover, heat stress led to pathological lesions in the internal organs and affected the expression of some genes related to heat stress, including proapoptotic genes such as caspase8 and bax, inflammatory genes such as NF-κβ1, and heat shock protein such as HSP 70 in the bursal tissue. These bad effects and abnormalities were mitigated by Ruminococcus alone or with enzyme supplementation, which improved all the above-mentioned parameters. Frontiers Media S.A. 2021-06-29 /pmc/articles/PMC8275643/ /pubmed/34268345 http://dx.doi.org/10.3389/fvets.2021.663577 Text en Copyright © 2021 Saad, Ahmed, Aboubakr, Ghoneim, Abdel-Daim, Albadrani, Arafat, Fadl and Abdo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Saad, Adel Hassan Ahmed, Mohamed S. Aboubakr, Mohamed Ghoneim, Hanan A. Abdel-Daim, Mohamed M. Albadrani, Ghadeer M. Arafat, Nagah Fadl, Sabreen Ezzat Abdo, Walied Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
title | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
title_full | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
title_fullStr | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
title_full_unstemmed | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
title_short | Impact of Dietary or Drinking Water Ruminococcus sp. Supplementation and/or Heat Stress on Growth, Histopathology, and Bursal Gene Expression of Broilers |
title_sort | impact of dietary or drinking water ruminococcus sp. supplementation and/or heat stress on growth, histopathology, and bursal gene expression of broilers |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275643/ https://www.ncbi.nlm.nih.gov/pubmed/34268345 http://dx.doi.org/10.3389/fvets.2021.663577 |
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