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Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance

Heroin use disorder is a chronic and relapsing disease that induces persistent changes in the brain. The diagnoses of heroin use disorders are mainly based on subjective reports and no valid biomarkers available. Recent researches have revealed that circulating miRNAs are useful non-invasive biomark...

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Autores principales: Liu, Haixiong, Xu, Wenjin, Feng, Jiying, Ma, Hong, Zhang, Jianbin, Xie, Xiaohu, Zhuang, Dingding, Shen, Wenwen, Liu, Huifen, Zhou, Wenhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275879/
https://www.ncbi.nlm.nih.gov/pubmed/34267687
http://dx.doi.org/10.3389/fpsyt.2021.679206
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author Liu, Haixiong
Xu, Wenjin
Feng, Jiying
Ma, Hong
Zhang, Jianbin
Xie, Xiaohu
Zhuang, Dingding
Shen, Wenwen
Liu, Huifen
Zhou, Wenhua
author_facet Liu, Haixiong
Xu, Wenjin
Feng, Jiying
Ma, Hong
Zhang, Jianbin
Xie, Xiaohu
Zhuang, Dingding
Shen, Wenwen
Liu, Huifen
Zhou, Wenhua
author_sort Liu, Haixiong
collection PubMed
description Heroin use disorder is a chronic and relapsing disease that induces persistent changes in the brain. The diagnoses of heroin use disorders are mainly based on subjective reports and no valid biomarkers available. Recent researches have revealed that circulating miRNAs are useful non-invasive biomarkers for diagnosing brain diseases such as Alzheimer's disease, multiple sclerosis, schizophrenia, and bipolar disorder. However, studies on circulating miRNAs for the diagnosis of heroin use disorders are rarely reported. In this study, we investigated the differential expression of plasma miRNAs in 57 heroin-dependent patients. Based on literature research and microarray analysis, two candidate miRNAs, miR-320a and let-7b-5p, were selected and analyzed by quantitative real-time RT-PCR. The results showed miR-320a and let-7b were significantly upregulated in plasma of the heroin-dependent patients compared to that in healthy controls. The area under curves (AUCs) of receiver operating characteristic (ROC) curves of miR-320a and let-7b-5p were 0.748 and 0.758, respectively. The sensitivities of miR-320a and let-7b-5p were 71.9 and 70.2%, while the specificities of miR-320a and let-7b-5p were 76.1 and 78.3%, respectively. The combination of these two miRNAs predicted heron dependence with an AUC of 0.782 (95% CI 0.687–0.876), with 73.7% sensitivity and 82.6% specificity. Our findings suggest a potential use for circulating miRNAs as biomarkers for the diagnosis of heroin abuse.
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spelling pubmed-82758792021-07-14 Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance Liu, Haixiong Xu, Wenjin Feng, Jiying Ma, Hong Zhang, Jianbin Xie, Xiaohu Zhuang, Dingding Shen, Wenwen Liu, Huifen Zhou, Wenhua Front Psychiatry Psychiatry Heroin use disorder is a chronic and relapsing disease that induces persistent changes in the brain. The diagnoses of heroin use disorders are mainly based on subjective reports and no valid biomarkers available. Recent researches have revealed that circulating miRNAs are useful non-invasive biomarkers for diagnosing brain diseases such as Alzheimer's disease, multiple sclerosis, schizophrenia, and bipolar disorder. However, studies on circulating miRNAs for the diagnosis of heroin use disorders are rarely reported. In this study, we investigated the differential expression of plasma miRNAs in 57 heroin-dependent patients. Based on literature research and microarray analysis, two candidate miRNAs, miR-320a and let-7b-5p, were selected and analyzed by quantitative real-time RT-PCR. The results showed miR-320a and let-7b were significantly upregulated in plasma of the heroin-dependent patients compared to that in healthy controls. The area under curves (AUCs) of receiver operating characteristic (ROC) curves of miR-320a and let-7b-5p were 0.748 and 0.758, respectively. The sensitivities of miR-320a and let-7b-5p were 71.9 and 70.2%, while the specificities of miR-320a and let-7b-5p were 76.1 and 78.3%, respectively. The combination of these two miRNAs predicted heron dependence with an AUC of 0.782 (95% CI 0.687–0.876), with 73.7% sensitivity and 82.6% specificity. Our findings suggest a potential use for circulating miRNAs as biomarkers for the diagnosis of heroin abuse. Frontiers Media S.A. 2021-06-29 /pmc/articles/PMC8275879/ /pubmed/34267687 http://dx.doi.org/10.3389/fpsyt.2021.679206 Text en Copyright © 2021 Liu, Xu, Feng, Ma, Zhang, Xie, Zhuang, Shen, Liu and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Psychiatry
Liu, Haixiong
Xu, Wenjin
Feng, Jiying
Ma, Hong
Zhang, Jianbin
Xie, Xiaohu
Zhuang, Dingding
Shen, Wenwen
Liu, Huifen
Zhou, Wenhua
Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance
title Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance
title_full Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance
title_fullStr Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance
title_full_unstemmed Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance
title_short Increased Expression of Plasma miRNA-320a and let-7b-5p in Heroin-Dependent Patients and Its Clinical Significance
title_sort increased expression of plasma mirna-320a and let-7b-5p in heroin-dependent patients and its clinical significance
topic Psychiatry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8275879/
https://www.ncbi.nlm.nih.gov/pubmed/34267687
http://dx.doi.org/10.3389/fpsyt.2021.679206
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