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Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo

Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and accounts for the fourth leading cause of all cancer deaths. Scientific evidence has found that plant extracts seem to be a reliable choice due to their multitarget effects against HCC. Juniperus communis has been used...

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Autores principales: Huang, Nan-Chieh, Huang, Ru-Lai, Huang, Xiao-Fan, Chang, Kai-Fu, Lee, Chien-Ju, Hsiao, Chih-Yen, Lee, Shan-Chih, Tsai, Nu-Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276093/
https://www.ncbi.nlm.nih.gov/pubmed/34151367
http://dx.doi.org/10.1042/BSR20211143
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author Huang, Nan-Chieh
Huang, Ru-Lai
Huang, Xiao-Fan
Chang, Kai-Fu
Lee, Chien-Ju
Hsiao, Chih-Yen
Lee, Shan-Chih
Tsai, Nu-Man
author_facet Huang, Nan-Chieh
Huang, Ru-Lai
Huang, Xiao-Fan
Chang, Kai-Fu
Lee, Chien-Ju
Hsiao, Chih-Yen
Lee, Shan-Chih
Tsai, Nu-Man
author_sort Huang, Nan-Chieh
collection PubMed
description Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and accounts for the fourth leading cause of all cancer deaths. Scientific evidence has found that plant extracts seem to be a reliable choice due to their multitarget effects against HCC. Juniperus communis has been used for centuries in traditional medicine and its anticancer properties have been reported. As a result, the purpose of the study was to investigate the anticancer effect and mechanism of J. communis extract (JCo extract) on HCC in vitro and in vivo. In the present study, we found that JCo extract inhibited the growth of human HCC cells by inducing cell cycle arrest at the G(0)/G(1) phase, extensive apoptosis and suppressing metastatic protein expressions in HCC cells. Moreover, the combinational treatment of JCo and VP-16 was found to enhance the anticancer effect, revealing that JCo extract might have the potential to be utilized as an adjuvant to promote HCC treatment. Furthermore, in vivo study, JCo extract significantly suppressed HCC tumor growth and extended the lifespan with no or low systemic and pathological toxicity. JCo extract significantly up-regulated the expression of pro-apoptotic proteins and tumor suppressor p53, suppressed VEGF/VEGFR autocrine signaling, down-regulated cell cycle regulatory proteins and MMP2/MMP9 proteins. Overall, our results provide a basis for exploiting JCo extract as a potential anticancer agent against HCC.
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spelling pubmed-82760932021-07-26 Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo Huang, Nan-Chieh Huang, Ru-Lai Huang, Xiao-Fan Chang, Kai-Fu Lee, Chien-Ju Hsiao, Chih-Yen Lee, Shan-Chih Tsai, Nu-Man Biosci Rep Cancer Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer and accounts for the fourth leading cause of all cancer deaths. Scientific evidence has found that plant extracts seem to be a reliable choice due to their multitarget effects against HCC. Juniperus communis has been used for centuries in traditional medicine and its anticancer properties have been reported. As a result, the purpose of the study was to investigate the anticancer effect and mechanism of J. communis extract (JCo extract) on HCC in vitro and in vivo. In the present study, we found that JCo extract inhibited the growth of human HCC cells by inducing cell cycle arrest at the G(0)/G(1) phase, extensive apoptosis and suppressing metastatic protein expressions in HCC cells. Moreover, the combinational treatment of JCo and VP-16 was found to enhance the anticancer effect, revealing that JCo extract might have the potential to be utilized as an adjuvant to promote HCC treatment. Furthermore, in vivo study, JCo extract significantly suppressed HCC tumor growth and extended the lifespan with no or low systemic and pathological toxicity. JCo extract significantly up-regulated the expression of pro-apoptotic proteins and tumor suppressor p53, suppressed VEGF/VEGFR autocrine signaling, down-regulated cell cycle regulatory proteins and MMP2/MMP9 proteins. Overall, our results provide a basis for exploiting JCo extract as a potential anticancer agent against HCC. Portland Press Ltd. 2021-07-12 /pmc/articles/PMC8276093/ /pubmed/34151367 http://dx.doi.org/10.1042/BSR20211143 Text en © 2021 The Author(s). https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Cancer
Huang, Nan-Chieh
Huang, Ru-Lai
Huang, Xiao-Fan
Chang, Kai-Fu
Lee, Chien-Ju
Hsiao, Chih-Yen
Lee, Shan-Chih
Tsai, Nu-Man
Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo
title Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo
title_full Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo
title_fullStr Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo
title_full_unstemmed Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo
title_short Evaluation of anticancer effects of Juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo
title_sort evaluation of anticancer effects of juniperus communis extract on hepatocellular carcinoma cells in vitro and in vivo
topic Cancer
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8276093/
https://www.ncbi.nlm.nih.gov/pubmed/34151367
http://dx.doi.org/10.1042/BSR20211143
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